Anthranilic acid from <i>Ralstonia solanacearum</i> plays dual roles in intraspecies signalling and inter-kingdom communication

Song, Shihao; Yin, Wenfang; Sun, Xiaoning; Cui, Binbin; Huang, Lei; Yang, Liang; Zhou, Jianuan; Deng, Yinyue

doi:10.1038/s41396-020-0682-7

Cited by 30 publications

(38 citation statements)

References 52 publications

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“…Indole and anthranilate are important intermediates for the biosynthesis of alkaloid compounds. Although the absolute abundance and ecophysiological role of indole and its derivatives in methanogenic ecosystems, including our UASB reactor, remain unknown, previous studies reported that these compounds are associated with bacterial signaling in Escherichia coli and Ralstonia solanacearum ( Pinero-Fernandez et al , 2011 ; Song et al , 2020 ). PRPP may be further converted to His through a 10-step reaction employing ATP, Glu, and NAD + .…”

Section: Resultsmentioning

confidence: 99%

Ecogenomics Reveals Microbial Metabolic Networks in a Psychrophilic Methanogenic Bioreactor Treating Soy Sauce Production Wastewater

et al. 2021

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An ecogenomic analysis of the methanogenic microbial community in a laboratory-scale up-flow anaerobic sludge blanket (UASB) reactor treating soy sauce-processing wastewater revealed a synergistic metabolic network. Granular sludge samples were collected from the UASB reactor operated under psychrophilic (20°C) conditions with a COD removal rate >75%. A 16S rRNA gene amplicon sequencing-based microbial community analysis classified the major microbial taxa as Methanothrix, Methanobacterium, Pelotomaculaceae, Syntrophomonadaceae, Solidesulfovibrio, and members of the phyla Synergistota and Bacteroidota. Draft genomes of dominant microbial populations were recovered by metagenomic shotgun sequencing. Metagenomic-and metatranscriptomic-assisted metabolic reconstructions indicated that Synergistotaand Bacteroidota-related organisms play major roles in the degradation of amino acids. A metagenomic bin of the uncultured Bacteroidales 4484-276 clade encodes genes for proteins that may function in the catabolism of phenylalanine and tyrosine under microaerobic conditions. Syntrophomonadaceae and Pelotomaculaceae oxidize fatty acid byproducts presumably derived from the degradation of amino acids in syntrophic association with aceticlastic and hydrogenotrophic methanogen populations. Solidesulfovibrio organisms are responsible for the reduction of sulfite and may support the activity of hydrogenotrophic methanogens and other microbial populations by providing hydrogen and ammonia using nitrogen fixation-related proteins. Overall, functionally diverse anaerobic organisms unite to form a metabolic network that performs the complete degradation of amino acids in the psychrophilic methanogenic microbiota.

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Section: Resultsmentioning

confidence: 99%

Ecogenomics Reveals Microbial Metabolic Networks in a Psychrophilic Methanogenic Bioreactor Treating Soy Sauce Production Wastewater

et al. 2021

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“…Previous studies showed that R. solanacearum uses two different types of QS systems: a phc QS system with 3-OH PAME and 3-OH MAME as signals and a typical lux QS system dependent on AHL signals. Our recent study identified a new signalling system, the anthranilic acid system, which controls important biological functions and the production of 3-OH MAME and AHL signals in R. solanacearum GMI1000 19 . In this study, we found that PutA positively controls the production of 3-OH MAME, AHL and anthranilic acid signals by directly controlling the transcriptional levels of the signal synthase-encoding genes by binding to the similar sequences in these promoters (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Escherichia coli strains that can be used for general cloning and conjugal transfer were cultured in Luria-Bertani medium (10 g/L tryptone, 5 g/L yeast extract, 5 g/L NaCl; pH 7.0-7.5) or on LB agar (LB medium containing 15 g/L agar) at 37 °C. In the EPS quantitative experiment, SP medium (5 g/L peptone, 20 g/L glucose, 0.5 g/L KH 2 PO 4 and 0.25 g/L MgSO 4 ) was used to culture R. solanacearum 19 . All media were supplemented with antibiotics according to the experimental requirements.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies showed that R. solanacearum utilizes at least two different types of quorum sensing (QS) systems, including the methyl 3-hydroxypalmitate (3-OH PAME) 16 or methyl 3-hydroxymyristate (3-OH MAME) system 17 , and the N-acylhomoserine lactone (AHL) system 18 , to control important biological functions. Furthermore, the anthranilic acid signalling system also plays a vital role to control the biological functions 19 . Among these systems, both the 3-OH PAME/3-OH MAME and anthranilic acid signalling systems modulate virulence factor production, motility activity and biofilm formation 16 – 23 .…”

Section: Introductionmentioning

confidence: 99%

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Proline utilization A controls bacterial pathogenicity by sensing its substrate and cofactors

Cui

et al. 2022

Commun Biol

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Previous reports indicate that proline utilization A (PutA) is involved in the oxidation of proline to glutamate in many bacteria. We demonstrate here that in addition to its role in proline catabolism, PutA acts as a global regulator to control the important biological functions and virulence of Ralstonia solanacearum. PutA regulates target gene expression levels by directly binding to promoter DNA, and its regulatory activity is enhanced by L-proline. Intriguingly, we reveal that the cofactors NAD+ and FAD boost the enzymatic activity of PutA for converting L-proline to L-glutamic acid but inhibit the regulatory activity of PutA for controlling target gene expression. Our results present evidence that PutA is a proline metabolic enzyme that also functions as a global transcriptional regulator in response to its substrate and cofactors and provide insights into the complicated regulatory mechanism of PutA in bacterial physiology and pathogenicity.

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“…at the root-microbiome interface can help shield the plant from pathogen infection [80,83,84]. Other mechanisms involved in the observed pathogen control might involve quorum sensing (QS) signals, siderophore-mediated interactions and systemically induced root exudation of metabolites (SIREM) [85][86][87]. Here, we report that the inoculation of speci c biological control agents can help stimulate speci c bene cial plant-associated microbes that together have the potential to protect the plants against pathogen attack.…”

Section: Impact Of Biocontrol Agent On the Resident Soil Community Asmentioning

confidence: 98%

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Tao

Xiong

et al. 2020

Preprint

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Background: Plant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms ( i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least in part due to impacts on the structure and function of the resident soil microbiome. However, it remains unclear whether such improvements are driven by the specific action of microbial inoculants, microbial populations naturally resident to the organic fertilizer or the physical-chemical properties of the compost substrate. The aim of this study was to seek the ecological mechanisms involved in the disease suppressive activity of bio-organic fertilizers. Results: To disentangle the mechanism of bio-organic fertilizer action, we conducted an experiment tracking Fusarium wilt disease of banana and changes in soil microbial communities over three growth seasons in response to the following four treatments: bio-organic fertilizer (containing Bacillus amyloliquefaciens W19), organic fertilizer, sterilized organic fertilizer and sterilized organic fertilizer supplemented with B. amyloliquefaciens W19. We found that sterilized bioorganic fertilizer to which Bacillus was re-inoculated provided a similar degree of disease suppression as the non-sterilized bioorganic fertilizer across cropping seasons. We further observed that disease suppression in these treatments is linked to impacts on the resident soil microbial communities, specifically by leading to increases in specific Pseudomonas spp.. Observed correlations between Bacillus amendment and indigenous Pseudomonas spp. that might underlie pathogen suppression were further studied in laboratory and pot experiments. These studies revealed that specific bacterial taxa synergistically increase biofilm formation and likely acted as a plant-beneficial consortium against the pathogen. Conclusion: Together we demonstrate that the action of bioorganic fertilizer is a product of the biocontrol inoculum within the organic amendment and its impact on the resident soil microbiome. This knowledge should help in the design of more efficient biofertilizers designed to promote soil function.

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Anthranilic acid from Ralstonia solanacearum plays dual roles in intraspecies signalling and inter-kingdom communication

Cited by 30 publications

References 52 publications

Ecogenomics Reveals Microbial Metabolic Networks in a Psychrophilic Methanogenic Bioreactor Treating Soy Sauce Production Wastewater

Ecogenomics Reveals Microbial Metabolic Networks in a Psychrophilic Methanogenic Bioreactor Treating Soy Sauce Production Wastewater

Proline utilization A controls bacterial pathogenicity by sensing its substrate and cofactors

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

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