Microbial
reductive dechlorination of polychlorinated biphenyls
(PCBs) is regarded as an alternative approach for in situ remediation and detoxification in the environment. To better understand
the process of PCB dechlorination in freshwater lake sediment, a long-term
(108 weeks) dechlorination study was performed in Taihu Lake sediment
microcosms with nine parent PCB congeners (PCB5, 12, 64, 71, 105,
114, 149, 153, and 170). Within 108 weeks, the total PCBs declined
by 32.8%, while parent PCBs declined by 84.8%. PCB dechlorinators
preferred to attack meta- and para-chlorines, principally para-flanked meta and single-flanked para chlorines. A total of
58 dechlorination pathways were observed, and 20 of them were not
in 8 processes, suggesting the broad spectrum of PCB dechlorination
in the environment. Rare ortho dechlorination was
confirmed to target the unflanked ortho chlorine,
indicating a potential for complete dechlorination. PCBs drove the
shifts of the microbial community structures, and putative dechlorinating
bacteria were growth-linked to PCB dechlorination. The distinct jump
of RDase genes ardA, rdh12, pcbA4, and pcbA5 was found to be consistent
with the commencement of dechlorination. The maintained high level
of putative dechlorinating phylum Chloroflexi (including Dehalococcoides and o-17/DF-1),
genus Dehalococcoides, and four RDase
genes at the end of incubation revealed the long-term dechlorination
potential. This work provided insights into dechlorination potential
for long-term remediation strategies at PCB-contaminated sites.