Anthurium in vitro: A review

Silva, Jaime A. Teixeira da; Dobránszki, Judit; Winarto, Budi; Zeng, Songjun

doi:10.1016/j.scienta.2014.11.024

Cited by 24 publications

(17 citation statements)

References 88 publications

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“…In vitro propagation is usually practiced for this particular plant. Therefore, a good number of protocols have been developed for various explants (Pierik et al 1974;Kunisaki 1980;Kuehnle and Sugii 1991;Matsumoto and Kuehnle 1997;Martin et al 2003;Viégas et al 2007;Beyramizade et al 2008;Winarto et al 2011a, b) but somatic embryogenesis is less commonly reported in A. andraeanum (Teixeira da Silva et al 2015). Kuehnle et al (1992) firstly reported somatic embryogenesis from leaf blade-derived embryogenic calli, and the histological evidence for bipolar structure of somatic embryos (SEs) and their origin was put forward by Matsumoto et al (1996).…”

Section: Introductionmentioning

confidence: 99%

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Bhattacharya

Dam

Karmakar

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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We present here a method for the in vitro propagation of Anthurium andraeanum cv. Fantasia through direct somatic embryogenesis. The embryos were directly formed at the cut end of most of the leaf explants when cultured on MS basal medium supplemented with N 6 -benzyladenine (BA) plus α-naphthalene acetic acid (NAA). MS basal media supplemented with BA (0.27 μM) and NAA (2.68 μM) induced maximum number of somatic embryos per leaf explant (15.33 ± 1.45) after 28 d of continuous culture. The highest numbers of embryos (12.33 ± 0.88) were matured into plantlets in MS basal medium augmented with 0.27 μM BA and 58.4 mM sucrose. Histology showed the presence of scutellum, coleoptile, welldeveloped root, and shoot initials at different stages of somatic embryo (SE) development directly on leaf explants. About 85% of the plantlets were successfully acclimatized, and of these, 80.3% plants survived after secondary hardening under greenhouse conditions. The embryo-derived plants successfully flowered. The presence of monomorphic DNA bands in random amplified polymorphic DNA (RAPD) marker analysis confirmed the genetic homogeneity of direct somatic embryo-derived plantlets in respect to their mother plant.

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Section: Introductionmentioning

confidence: 99%

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Bhattacharya

Dam

Karmakar

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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“…It is well known that the success of morphogenesis is due, in part, to the correct selection of the type and concentration of the PGR. Teixeira et al (2015) reported that the adequate concentration of PGRs or their combinations differed for different varieties, explants or culture stages, including, most importantly, the differentiation and proliferation of shoots for callus induction, formation of roots and other organogenic processes. In this report, TDZ was evaluated given that it has demonstrated its effectiveness in the induction of morphogenesis (Gopale et al, 2013).…”

Section: Morphogenesis and Plant Regenerationmentioning

confidence: 99%

Morphogenesis and plant regeneration from Anthurium andreanum cv Calypso leaf explant

Guadalupe¹,

Mayanin²

2017

Afr. J. Biotechnol.

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Anthuriums are among the most attractive ornamental plants; however, the commercial production of these plants is limited by the slow propagation methods presently in use. This situation can be resolved with the application of in vitro culture techniques which allow massive plant propagation through morphogenic processes. Plant growth regulators (PGR) and the composition of the basal media comprising the culture medium are among the factors influencing the induction of morphogenesis. Optical and electron microscopy analysis suggested that the morphogenic routes induced were organogenesis and somatic embryogenesis. This report presents three protocols of morphogenesis, two for adventitious shoot organogenesis and one via somatic embryogenesis. The treatments which induced adventitious shoot organogenesis were Murashige and Skoog medium at half ionic strength supplemented with 0.2 μM of thidiazuron (TDZ) and 2.2 μM of 6-benzylaminopurine (BAP); 0.2 μM of TDZ and 6.6 μM of zeatin (ZEA); the treatment which induced somatic embryogenesis was 0.4 μM of TDZ and 0.5 μM of 2, 4-dichlorophenoxyacetic acid (2, 4-D).

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“…However, a successful genetic transformation protocol can only exist when the regeneration of transformed tissue in vitro is guaranteed. Therefore, the development of efficient tissue culture methods is crucial for each variety to be genetically transformed (Teixeira da Silva et al 2015a). The use of anther culture also holds great promise for the production of haploid plants and may serve as a valuable target tissue for genetic transformation studies (Teixeira da Silva et al 2015b).…”

Section: Importance Of Genetic Transformation In Anthuriummentioning

confidence: 99%

“…The target tissue itself used in Agrobacterium co-culture has varying responses and susceptibilities to transformation. Efforts are needed to systematically screen sources of undifferentiated tissues for better transformation rates provided that there is a well-established regeneration protocol available (Teixeira da Silva et al 2015a, b). One tissue worth considering for improved transformation efficiency is embryogenic cell suspension cultures.…”

Section: Manipulation Of Flower Colourmentioning

confidence: 99%

Genetic transformation and molecular research in Anthurium: progress and prospects

Silva

Dobránszki

Zeng

et al. 2015

Plant Cell Tiss Organ Cult

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Although Anthurium is an attractive and commercially popular ornamental plant, its genetic enhancement has lagged behind that of other ornamental crops. There are several agronomically important traits in need of improvement. These include novel flower colors and morphologies, increased shelf and vase lives, and resistance to bacterial blight (Xanthomonas axonopodis pv. dieffenbachiae), burrowing nematodes and abiotic stresses.

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Anthurium in vitro: A review

Cited by 24 publications

References 88 publications

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Morphogenesis and plant regeneration from Anthurium andreanum cv Calypso leaf explant

Genetic transformation and molecular research in Anthurium: progress and prospects

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