Anti-a and Anti-B Blood Group Antibody Levels in Relation to Age in Cynomolgus Monkeys

Terao, Keiji; Fujimoto, Koji; Cho, Fumiaki; Honjo, Shigeo

doi:10.7883/yoken1952.36.289

Cited by 8 publications

(9 citation statements)

References 13 publications

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“…Therefore, alternative method was necessary and we adopted indirect method in determining the blood type by monitoring reactivity of agglutination after serum of Cynomolgus monkey was mixed with human RBC. However, this antisera of this test has a drawback from age-associated differences in the levels of serum antibody secreted in each animal [ 27 ]. Therefore, it is necessary to other validation support such as DNA analysis and immunohistochemistry (IHC) for determining the blood type of Cynomolgus monkeys [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Reference values of hematology, biochemistry, and blood type in cynomolgus monkeys from cambodia origin

Choi¹,

Chang²,

Lee

et al. 2016

Lab Anim Res

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Cynomolgus monkeys as nonhuman primates are valuable animal models because they have a high level of human gene homology. There are many reference values for hematology and biochemistry of Cynomolgus monkeys that are needed for proper clinical diagnosis and biomedical research conduct. The body weight information and blood type are also key success factors in allogeneic or xenogeneic models. Moreover, the biological parameters could be different according to the origin of the Cynomolgus monkey. However, there are limited references provided, especially of Cambodia origin. In this study, we measured average body weight of 2,518 Cynomolgus monkeys and analyzed hematology and serum biochemistry using 119 males, and determined blood types in 642 monkeys with Cambodia origin. The average body weight of male Cynomolgus monkeys were 2.56±0.345 kg and female group was 2.43±0.330 kg at the age from 2 to 3 years. The male group showed relatively sharp increased average body weight from the 3 to 4 age period compared to the female group. In hematology and biochemistry, it was found that most of the data was similar when compared to other references even though some results showed differences. The ABO blood type result showed that type A, B, AB, and O was approximately 15.6, 33.3, 44.2, and 6.9%, respectively. The main blood type in this facility was B and AB. These biological background references of Cambodia origin could be used to provide important information to researchers who are using them in their biomedical research.

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Section: Discussionmentioning

confidence: 99%

Reference values of hematology, biochemistry, and blood type in cynomolgus monkeys from cambodia origin

Choi¹,

Chang²,

Lee

et al. 2016

Lab Anim Res

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“…5,[10][11][12][13] Premasuthan et al 5,10 reported the distribution of the A, B and AB phenotypes in relatively small samples of captive cynomolgus and rhesus macaques based on the SNP analysis. While Premasuthan et al's 5,10 conclusions regarding phenotype frequencies were based on more animals than previous reports, 8,11,14,15 an even larger sampling of rhesus and cynomolgus macaques is needed to confirm the accuracy of the phenotype frequency estimates reported in their studies. To characterize the frequency distribution of A, B and AB phenotypes in cynomolgus and rhesus macaques, this study analyzed the phenotypic data from 2369 animals.…”

mentioning

confidence: 84%

“…While Premasuthan et al’s [2011 [2012] conclusions regarding phenotype frequencies were based on more animals than previous reports [Doxiadis et al, 1998; Malaivijitnond et al, 2008; Nakajima and Ogushi, 1965; Terao et al, 1983], an even larger sampling of rhesus and cynomolgus macaques is needed to confirm the accuracy of the phenotype frequency estimates reported in their studies. To characterize the frequency distribution of A, B and AB phenotypes in cynomolgus and rhesus macaques, this study analyzed the phenotypic data from 2369 animals.…”

Section: Introductionmentioning

confidence: 94%

ABO blood group phenotype frequency estimation using molecular phenotyping in rhesus and cynomolgus macaques

Kanthaswamy

Oldt

et al. 2017

HLA

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A much larger sample (N = 2369) was used to evaluate a previously reported distribution of the A, AB and B blood group phenotypes in rhesus and cynomolgus macaques from six different regional populations. These samples, acquired from 15 different breeding and research facilities in the US, were analyzed using a real-time quantitative polymerase chain reaction (qPCR) assay that targets single nucleotide polymorphisms (SNPs) responsible for the macaque A, B and AB phenotypes. The frequency distributions of blood group phenotypes of the two species differ significantly from each other, and significant regional differentiation within the geographic ranges of each species was also observed. The B blood group phenotype was prevalent in rhesus macaques, especially those from India, while the frequencies of the A, B and AB phenotypes varied significantly among cynomolgus macaques from different geographic regions. The Mauritian cynomolgus macaques, despite having originated in Indonesia, showed significant (p ≪ 0.01) divergence from the Indonesian animals at the ABO blood group locus. Most Mauritian animals belonged to the B blood group while the Indonesian animals were mostly A. The close similarity in blood group frequency distributions between the Chinese rhesus and Indochinese cynomolgus macaques demonstrates that the introgression between these two species extends beyond the zone of intergradation in Indochina. This study underscores the importance of ABO blood group phenotyping of the domestic supply of macaques and their biospecimens.

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“…Both circumstances can lead to false‐positive results and underestimates of the O phenotype. Moreover, the concentration of ABH antigens or antibodies in tissues is highly variable and, in the case of antibodies, decline with age , occasionally providing ambiguous agglutination results. These ambiguities are exacerbated by the weak secretor status of most macaques, potentially leading to the erroneous assignment of O phenotypes.…”

Section: Introductionmentioning

confidence: 99%

Molecular ABO phenotyping in cynomolgus macaques using real‐time quantitative PCR

Premasuthan

Kanthaswamy

et al. 2012

Tissue Antigens

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Macaques are commonly used in biomedical research as animal models of human disease. The ABO phenotype of donors and recipients plays an important role in the success of transplantation and stem cell research of both human and macaque tissue. Traditional serological methods for ABO phenotyping can be time consuming, provide ambiguous results and/or require tissue that is unavailable or unsuitable. We developed a novel method to detect the A, B, and AB phenotypes of macaques using real-time quantitative PCR. This method enables the simple and rapid screening of these phenotypes in macaques without the need for fresh blood or saliva. This study reports the distribution of the A, B, and AB phenotypes of captive cynomolgus macaques that, while regionally variable, closely resembles that of rhesus macaques. Blood group B, as in rhesus macaques, predominates in cynomolgus macaques and its frequency distribution leads to a probability of major incompatibility of 41%. No silencing mutations have been identified in exons 6 or 7 in macaques that could be responsible for the O phenotype, that, although rare, have been reported. The excess homozygosity of rhesus and cynomolgus macaque genotypes in the present study, that assumes the absence of the O allele, suggests the possibility of some mechanism preventing the expression of the A and B transferases.

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Anti-a and Anti-B Blood Group Antibody Levels in Relation to Age in Cynomolgus Monkeys

Cited by 8 publications

References 13 publications

Reference values of hematology, biochemistry, and blood type in cynomolgus monkeys from cambodia origin

Reference values of hematology, biochemistry, and blood type in cynomolgus monkeys from cambodia origin

ABO blood group phenotype frequency estimation using molecular phenotyping in rhesus and cynomolgus macaques

Molecular ABO phenotyping in cynomolgus macaques using real‐time quantitative PCR

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