Anti-adhesion and Anti-inflammatory Potential of the Leaderless Class IIb Bacteriocin Enterocin DD14

Abstract

In this study, we investigate the interactions between the leaderless class IIb bacteriocin, enterocin DD14 (EntDD14), or the methicillin or the combination of these antibacterials, and two methicillin-resistant Staphylococcus aureus strains (MRSA-S1 and USA 300) which are respectively a clinical strain and a reference strain. The results obtained showed that EntDD14 alone or in combination with the antibiotic could significantly prevent the adhesion of these pathogenic bacteria to human cells. On the other hand, we investigated the anti-inflammatory effect of EntDD14 on the secretion of pro-inflammatory interleukins, including IL-6 and IL-8. The results show that EntDD14 is able to decrease significantly the secretion of both interleukins on Caco-2 cells following their treatments with lipopolysaccharides. These novel data provide insightful informations to support applications of bacteriocins as therapeutic agents capable as well to defeat pathogenic bacteria and concomitantly limit their inflammatory reactions.

Immunomodulation, Bioavailability and Safety of Bacteriocins

“…This bacteriocin has been characterized as a potent chemical inhibitor of the The enterocin DD14 (Ent DD14), which is produced by Enterococcus faecalis 14 strain isolated from newborn's meconium, exerts the anti-inflammatory effect on the secretion of pro-inflammatory interleukins, including IL-6 and IL-8. The results show that EntDD14 is able to significantly decrease the secretion of both interleukins on Caco-2 cells following their treatments with LPS [117].…”

A Review on Enterocin DD14, the Leaderless Two-Peptide Bacteriocin with Multiple Biological Functions and Unusual Transport Pathway

“…It is of note that treatment of C. perfringens with such nanobiotics (bacteriocins loaded on alginate nanoparticles) has annihilated the expression of genes encoding some virulence factors [76]. Recently, a study carried out in our laboratory has established an anti-adhesive action of EntDD14 against several MRSA strains, noticeably across S. aureus USA300, which is known for its virulence traits and ability to colonize different surfaces [87]. In this study, we also unveiled the potential of EntDD14 to decrease the secretion of pro-inflammatory interleukins, IL-6 and IL-8, on human intestinal Caco-2 cells previously inflamed with bacterial lipopolysaccharide [87].…”

“…Recently, a study carried out in our laboratory has established an anti-adhesive action of EntDD14 against several MRSA strains, noticeably across S. aureus USA300, which is known for its virulence traits and ability to colonize different surfaces [87]. In this study, we also unveiled the potential of EntDD14 to decrease the secretion of pro-inflammatory interleukins, IL-6 and IL-8, on human intestinal Caco-2 cells previously inflamed with bacterial lipopolysaccharide [87]. Generally active only against closely phylogenetically related microorganism, some LAB-bacteriocins, however, are exceptionally active against Gram-negative bacilli [88,89].…”

“…Bacteriocins have already been described for their antiviral activity [87,91], and these include EntCRL35 or GEn17 on Herpes Simplex Virus-1 (HSV-1) [92,93]. Moreover, a study based on a global simulation approach established that bacteriocins warrant more attention for their potential against SARS-CoV-2 viruses [94].…”