Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

Kim, Suk-Jin; Choi, Soo‐Im; Jang, Miran; Jeong, Yulah; Kang, Chang-Ho; Kim, Gun-Hee

doi:10.1007/s10068-020-00819-2

Cited by 22 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To confirm this antiadipogenic effect, the expression of adipogenesis-related genes was examined by qRT-PCR. Several studies have demonstrated the inhibition of adipogenesis in adipocytes on treatment with functional bacterial strains, which regulate adipogenic transcription factors [35,36]. Similar findings were observed in our study.…”

Section: Discussionsupporting

confidence: 92%

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Lee

Park²,

Kim

2021

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Abnormal adipocyte growth, distinguished by an increase in cell numbers and cellular differentiation, is regarded as a major pathological characteristic of obesity. Thus, inhibition of adipogenic differentiation in adipocytes could prevent obesity. Recently, certain probiotic stains have been reported to regulate lipid metabolism in vitro and/or in vivo. In this backdrop, this study aimed to investigate basic probiotic properties and potential antiobesity characteristics of mouse 3T3-L1 preadipocytes. Six lactic acid bacteria (LAB) strains were prescreened for their cholesterol-lowering activity, antioxidant activity, and survival at low pH and in a solution containing bile salts. These six strains were investigated for antiadipogenic activity by employing 3T3-L1 mouse preadipocytes. 3T3-L1 cells were treated with selected strains during the differentiation process. Lactobacillus johnsonii 3121 and Lactobacillus rhamnosus 86 were found to be more capable of reducing triglyceride and lipid accumulation, as compared to control group, which are fully differentiated 3T3-L1 adipocytes. These strains also inhibited adipocyte differentiation by downregulating the adipogenic transcription factor in 3T3-L1 adipocytes. Taken together, these results indicate that L. johnsonni 3121 and L. rhamnosus 86 could potentially act as probiotic bacteria and prevent fat accumulation by regulating adipogenesis-related markers.

show abstract

Section: Discussionsupporting

confidence: 92%

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Lee

Park²,

Kim

2021

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

show abstract

“…The adhesion ability of LAB was evaluated using HT-29 colorectal cells, as described previously [ 21 ]. HT-29 colorectal cells (1 × 10 5 cells/mL) were incubated in 12-well plates in 5% CO 2 at 37 °C for 24 h. The LAB were cultured in MRS broth at 37 °C for 24 h. The strains were resuspended at 1 × 10 8 CFU/mL in DMEM without FBS and PS and administered to cells.…”

Section: Methodsmentioning

confidence: 99%

Lactic Acid Bacteria Exert a Hepatoprotective Effect against Ethanol-Induced Liver Injury in HepG2 Cells

Lee¹,

Kim²,

Jeong³

et al. 2021

Microorganisms

Self Cite

View full text Add to dashboard Cite

Alcoholic liver fatty disease (ALFD) is caused by excessive and chronic alcohol consumption. Alcohol consumption causes an imbalance in the intestinal microflora, leading to liver disease induced by the excessive release of endotoxins into the hepatic portal vein. Therefore, research on the intestinal microflora to identify treatments for ALFD is increasing. In this study, the protective effects of lactic acid bacteria (LAB) strains, including Levilactobacillus brevis, Limosilactobacillus reuteri, and Limosilactobacillus fermentum, were evaluated in ethanol-induced HepG2 cells. Among the evaluated LAB, nine strains increased aldehyde dehydrogenase (ALDH) levels and downregulated lipid peroxidation and liver transferase in the ethanol-induced HepG2 cells. Moreover, L. brevis MG5280 and MG5311, L. reuteri MG5458, and L. fermentum MG4237 and MG4294 protected against ethanol-induced HepG2 cell damage by regulating CYP2E1, antioxidant enzymes (SOD, CAT, and GPX), lipid synthesis factors (SREBP1C and FAS), and lipid oxidation factors (PPARα, ACO, and CPT-1). Moreover, five LAB were confirmed to be safe probiotics based on antibiotic susceptibility and hemolysis assays; their stability and adhesion ability in the gastrointestinal tract were also established. In conclusion, L. brevis MG5280 and MG5311, L. reuteri MG5458, and L. fermentum MG4237 and MG4294 may be useful as new probiotic candidates for ALFD prevention.

show abstract

“…ND group received a diet containing 14% fat, 21% protein, and 64% carbohydrate (Furina Inc., Seongnam, Korea). Dosages of these strains were established based on results obtained from in vitro preliminary studies (Kim et al, 2020) and those used in previous studies (Hussain et al, 2020;Li et al, 2020).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…In our previous study, two human-derived strains of Limosilactobacillus (formerly Lactobacillus) fermentum MG4231 and MG4244 were isolated. These strains have been shown to inhibit adipocyte differentiation in vitro and have been identified as probiotics candidates Kim et al, 2020). Therefore, this study aimed to evaluate the anti-obesity activity of L. fermentum MG4231, MG4244, and their combination to identify the underlying molecular mechanisms associated with lipid metabolism in a HFD-induced obese mouse model.…”

Section: Introductionmentioning

confidence: 99%

Combination of Limosilactobacillus fermentum MG4231 and MG4244 attenuates lipid accumulation in high-fat diet-fed obese mice

Kim

Choi

Jang

et al. 2021

View full text Add to dashboard Cite

We investigated the anti-obesity effect and the underlying mechanisms of action of human-derived Limosilactobacillus fermentum MG4231, MG4244, and their combination, in high-fat diet-induced obese mice. Administration of the Limosilactobacillus strains decreased body weight gain, liver and adipose tissue weight, and glucose tolerance. Serum levels of total cholesterol, low-density lipoprotein-cholesterol, and leptin were reduced, while adiponectin increased. The administration of Limosilactobacillus strains improved the histopathological features of liver tissue, such as hepatic atrophy and inflammatory penetration, and significantly reduced the content of triglyceride in the liver. Limosilactobacillus administration discovered a significant reduction in the size of the adipocytes in the epididymal tissue. Limosilactobacillus treatment significantly reduced the expression of important regulators in lipid metabolism, including peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, fatty acid synthase (FAS), adipocyte-protein 2, and lipoprotein lipase in the epididymal tissue. Also, Limosilactobacillus lowered sterol regulatory element-binding protein 1-c and FAS in the liver tissue. Such changes in the expression of these regulators in both liver and epididymis tissue were caused by Limosilactobacillus upregulating phosphorylation of AMP-activated protein kinase and acetyl-CoA carboxylase. Therefore, we suggest that the use of the combination of L. fermentum MG4231 and MG4244, as probiotics could effectively inhibit adipogenesis and lipogenesis from preventing obesity.

show abstract

Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes

Cited by 22 publications

References 46 publications

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Lactic Acid Bacteria Exert a Hepatoprotective Effect against Ethanol-Induced Liver Injury in HepG2 Cells

Combination of Limosilactobacillus fermentum MG4231 and MG4244 attenuates lipid accumulation in high-fat diet-fed obese mice

Contact Info

Product

Resources

About