Anti-aging effect of riboflavin via endogenous antioxidant in fruit fly Drosophila melanogaster

Zou, Yingxin; Ruan, Ming-Hua; Luan, Jie; Feng, Xu; Chen, S.; Chu, Zun-hua

doi:10.1007/s12603-016-0752-8

Cited by 38 publications

(34 citation statements)

References 32 publications

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“…Oxidative stress is a key effect of aging and degenerative diseases along with aging. The authors have demonstrated that supplementation of RF significantly extended the lifetime and strengthened the reproduction of fruit flies via enhancing the activity of antioxidant enzymes [11]. RF also activates the synthesis of a normal extracellular matrix and reduces reactive oxygen species (ROS) levels in keratoconus [12].…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

“…Anti-aging in Drosophila melanogaster (fruit fly) RF at 120 µg/mL SOD1 ↑; CAT ↑; lipofuscin (LF) ↓ RF prolonged the life span and increased reproductive capacity through anti-oxidative stress pathway involving enhancing the activity of SOD1 and CAT and inhibiting lipofuscin accumulation [11] Keratoconus corneal stroma cells RF with selenium administration protected against GTN-induced brain oxidative toxicity by protecting brain MMCA activity, inhibiting free radicals and supporting the antioxidant redox system. [19] Migraine model RF 100 mg/kg was treated before GTN-induced migraine Lipid peroxidation↓, GSH↑ RF and vitamin E had a protective effect on the GTN-induced brain injury by inhibiting free radical production, regulation of calcium-dependent processes, and supporting the antioxidant redox system.…”

Section: Dose Antioxidant Enzymes Key Findings Referencesmentioning

confidence: 99%

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Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

250

107

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Riboflavin (RF) is a water-soluble member of the B-vitamin family. Sufficient dietary and supplemental RF intake appears to have a protective effect on various medical conditions such as sepsis, ischemia etc., while it also contributes to the reduction in the risk of some forms of cancer in humans. These biological effects of RF have been widely studied for their anti-oxidant, anti-aging, anti-inflammatory, anti-nociceptive and anti-cancer properties. Moreover, the combination of RF and other compounds or drugs can have a wide variety of effects and protective properties, and diminish the toxic effect of drugs in several treatments. Research has been done in order to review the latest findings about the link between RF and different clinical aberrations. Since further studies have been published in this field, it is appropriate to consider a re-evaluation of the importance of RF in terms of its beneficial properties.

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Section: Antioxidant Propertiesmentioning

confidence: 99%

Section: Dose Antioxidant Enzymes Key Findings Referencesmentioning

confidence: 99%

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

250

107

View full text Add to dashboard Cite

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“…Huangfu et al (2013) employed fruit fly model system to test the antiaging effects of astaxanthin-rich alga (Haematococcus pluvialis) in which it markedly enhanced their lifespan and reduced the age-related decline in locomotor function. Zou et al (2017) studied the antiaging effect of riboflavin in fruit fly model system and found that riboflavin significantly extended the lifespan and reproduction of fruit fly by elevating superoxide dismutase 1 (SOD1) and catalase (CAT). Moreover, fruit fly was used to demonstrate the improvement of methionine sulfoxide reductase activity under the oxidative stress conditions by feeding methyl-cysteine fortified meals (Wassef et al, 2007).…”

Section: Fruit Fly Modelmentioning

confidence: 99%

Should the in vitro colorimetric assays in antioxidant and lipid oxidation evaluation be abandoned? A critical review focusing on bioactive molecule screening assays in in vitro and in vivo models

Shahidi¹,

Yeo²

2020

Journal of Food Bioactives

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Increasing evidence has proven the potent antioxidative effectiveness of bioactives in natural products for preventing/suppressing chronic diseases. In this connection, the development of efficient methods that are suitable to screen bioactives in vitro and in vivo tests has taken place. Thus, a variety of assays have been used in the extraction of bioactives, testing of their antioxidant potential in both in vitro and in vivo model, and evaluating lipid oxidation are comprehensively discussed here. This review mainly focuses on the principle and the use of individual assays in both in vitro and in vivo models. Given that induvial assays have pros and cons due to the discrepancy in the reaction environment and applied biological system, application to the various assays in order to complement the drawbacks of each assay is highly recommended to obtain the reasonable information from experimental trials.

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“…Yet, the negative effect can be prevented by antioxidants. The combined vitamin B supplementation causes a marked decrease in ROS, protein oxidation, and an enhancement of antioxidative enzymes [9,[14][15][16]. Subjects continuously exposed to low-grade oxidative stress, such as manual laborers [17] and patients suffering from schizophrenia [18] or chronic illnesses including cancer, might particularly benefit from the antioxidant and anti-inflammatory impact of the vitamin B in the prevention of lipid peroxidation [19,20].…”

Section: Introductionmentioning

confidence: 99%

A Randomized Pilot Trial to Evaluate the Bioavailability of Natural versus Synthetic Vitamin B Complexes in Healthy Humans and Their Effects on Homocysteine, Oxidative Stress, and Antioxidant Levels

Lindschinger¹,

Tatzber

Schimetta

et al. 2019

Oxidative Medicine and Cellular Longevity

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The vitamin B complex comprises 8 different water-soluble constituents that humans must sequester from the diet. This pilot study compared natural versus synthetic vitamin B complexes for their bioavailability, accumulation, and their impact on antioxidants, homocysteine levels, and oxidative stress. We conducted a double-blind randomized clinical trial with thirty healthy participants. They were randomly assigned to group N (natural) and group S (synthetic). Vitamin B was ingested daily for 6 weeks in the range of about 2.5 times above the recommended daily allowance. Blood samples were taken at baseline, 1.5 h, 4 h, 7 h (diurnal), 6 w (discontinuation of supplements), and 8 w (washout). Blood levels of thiamine (B 1 ), riboflavin (B 2 ), pyridoxine (B 6 ), folic acid (B 9 ), cobalamin (B 12 ), homocysteine, total antioxidants, peroxidase activity, polyphenols, and total peroxides were determined. Compared to initial values, serum levels of each B vitamin increased at the end of the supplementation period: i.e., B 1 (+23% N; +27% S), B 2 (+14% N; +13% S), B 6 (+101% N; +101% S), B 9 (+86% N; +153% S), and B 12 (+16% N) (p < 0:05). Homocysteine (-13% N) decreased, while peroxidase activity (+41% S) and antioxidant capacity increased (+26% N). Short-term effects were already observed after 1.5 h for B 9 (+238% N; +246% S) and after 4 h for vitamin B 2 (+7% N; +8% S), B 6 (+59% N; +51% S), and peroxidase activity (+58% N; +58% S). During the washout period, serum levels of B vitamins decreased except for thiamine and peroxidase activity, which increased further. This clinical pilot study revealed comparable bioavailability for both natural and synthetic B vitamins but did not show statistically noticeable differences between groups despite some favourable tendencies within the natural vitamin group, i.e., sustained effects for cobalamin and endogenous peroxidase activity and a decrease in homocysteine and oxidative stress levels.

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Anti-aging effect of riboflavin via endogenous antioxidant in fruit fly Drosophila melanogaster

Abstract: Riboflavin prolonged the lifespan and increased the reproduction of fruit flies through anti-oxidative stress pathway involving enhancing the activity of SOD1 and CAT and inhibiting LF accumulation. Riboflavin deserves more attention for slowing human aging.

Cited by 38 publications

References 32 publications

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Should the in vitro colorimetric assays in antioxidant and lipid oxidation evaluation be abandoned? A critical review focusing on bioactive molecule screening assays in in vitro and in vivo models

A Randomized Pilot Trial to Evaluate the Bioavailability of Natural versus Synthetic Vitamin B Complexes in Healthy Humans and Their Effects on Homocysteine, Oxidative Stress, and Antioxidant Levels

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