Anti-allergic and anti-inflammatory effects of hydrosol extracted from Zanthoxylum schinifolium branch

Abstract

Zanthoxylum schinifolium Sieb. et Zucc. (syn. Fagara schinifolia Engler) was studied for its potential anti-inflammatory properties. The hydrosol extract prepared from the Z. schinifolium branch was analyzed by gas chromatography/mass spectrometry. Here, five main chemical components were identified in the hydrosol of the branches of this shrub. The main chemical compounds in the branch inhibited both an Immunoglobulin E (IgE)-antigen complex and a dinitrophenyl-bovine serum albumin (DNP-BSA)-induced β-hexosaminidase release in a dose-dependent manner in RBL-2H3 mast cells, and at the tested concentrations did not show cytotoxicity to RBL-2H3 cells. Moreover, hydrosol obtained from the branch substantially inhibited a lipopolysaccharide (LPS) induced overproduction of intracellular active oxygen (ROS) and nitric oxide (NO). Consistently, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) proteins of SNAP23, syntaxin4, VAMP7, and VAMP8 were remarkably decreased through hydrosol treatment. Hydrosol suppressed the activation of SNARE proteins in DNP-BSA-stimulated RBL-2H3 cells and inhibited ROS and NO in LPS-stimulated RAW264.7 cells. Camphor and estragole are the main chemical components of hydrosol and downregulate the LPS-induced phosphorylation of the SNARE proteins. The hydrosol obtained from the branch of Z. schinifolium has therapeutic benefits for allergic inflammatory diseases.

Disclosing the potential of Cupressus leylandii A.B. Jacks & Dallim, Eucalyptus globulus Labill., Aloysia citrodora Paláu, and Melissa officinalis L. hydrosols as eco-friendly antimicrobial agents

“…Hydrosols have recently started to receive a lot of credit in a variety of areas, such as food (e.g., flavouring, preservatives, and sanitisers) [ 18 , 19 ], cosmetic and perfumery [ 20 ], aromatherapy [ 21 ], agriculture (e.g., biopesticides and repellents) [ 22 ], pharmaceutical (e.g., natural antibiotics, antioxidants and anti-inflammatory agents) [ 17 , 23 ], and medical (e.g., antimicrobial and antitumor agents) [ 24 ]. According to circular economy principles, using industrial by-products can be a sustainable way to address the environmental problems caused by waste discharging.…”

Viola calcarata L. and Viola dubyana Burnat ex Gremli hydrolates: DI-SPME-GC-MS analysis and biological activity evaluation

“…Currently, many hydrolates are widely exploited in aromatherapy, cosmetic formulations, food industry and organic agriculture [Aćimović et al 2020a]. Several studies reported their antioxidant, anti-allergic, antiinflammatory antimicrobial and fungicidal properties as well as cytotoxic or anti-enzymatic activity [Boyraz & Ozcan 2006;Ko et al 2017;Shen et al 2017;D'Amato et al 2018;Mohamed Gameil et al 2019;Ha et al 2021;Pérez-Izquierdo et al 2022;Yu et al 2022]. Allelopathic effect was also studied to find alternative agricultural strategies, for example in weed control in order to reduce the use of traditional agrochemicals along with their side effects on the environment and human health.…”