Anti-Allergic Diarrhea Effect of Diosgenin Occurs via Improving Gut Dysbiosis in a Murine Model of Food Allergy

Huang, Chung-Hsiung; Pan, Chorng-Liang; Tsai, Guo-Jane; Chang, Chun–Ju; Tsai, Wei–Chung; Lu, Shueh-Yu

doi:10.3390/molecules26092471

Cited by 5 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Brandt and colleagues [28] described diarrhea after the 3rd and 4th OVA applications. From a total of 10 allergen challenges, Huang et al observed diarrhea symptoms not before the 6th one [71,72]. In a study examining the role of coumarin in OVA anaphylaxis, diarrhea score increased with ongoing allergen challenges [66].…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Treatment Prevents Increase of Mast Cells in Both Murine OVA Enteritis and IL-10−/− Colitis

Bilotta

Arbogast

Schart

et al. 2022

IJMS

View full text Add to dashboard Cite

Mast cells are involved in allergic and other inflammatory diseases. The polyphenol resveratrol is known for its anti-inflammatory properties and may be used as nutraceutical in mast cell associated diseases. We analyzed the effect of resveratrol on mast cells in vivo in ovalbumin-induced allergic enteritis as well as experimental colitis in IL-10−/− mice which received resveratrol via drinking water. Treatment with resveratrol prevented the increase in mast cells in both allergic enteritis and chronic colitis in duodenum as well as in colon. Further, it delayed the onset of diseases symptoms and ameliorated diseases associated parameters such as tissue damage as well as inflammatory cell infiltration in affected colon sections. In addition to the findings in vivo, resveratrol inhibited IgE-dependent degranulation and expression of pro-inflammatory cytokines such as TNF-α in IgE/DNP-activated as well as in LPS-activated bone marrow-derived mast cells. These results indicate that resveratrol may be considered as an anti-allergic and anti-inflammatory plant-derived component for the prevention or treatment of mast cell-associated disorders of the gastrointestinal tract.

show abstract

Section: Discussionmentioning

confidence: 99%

Resveratrol Treatment Prevents Increase of Mast Cells in Both Murine OVA Enteritis and IL-10−/− Colitis

Bilotta

Arbogast

Schart

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Based on the 16S rRNA sequencing results, reduced α- and β-diversity indices and altered gut microbiota composition were observed in diarrhea rabbits compared to healthy rabbits. This suggests that diarrhea may be linked to dynamic changes in the composition of the intestinal microbiota (Huang et al, 2021b ). Compared to healthy rabbits, diarrhea rabbits exhibited significantly higher proportions of the phyla Proteobacteria and Firmicutes, whereas significantly lower proportions of Bacteroidetes and Tenericutes.…”

Section: Discussionmentioning

confidence: 99%

Microbiome, Transcriptome, and Metabolomic Analyses Revealed the Mechanism of Immune Response to Diarrhea in Rabbits Fed Antibiotic-Free Diets

et al. 2022

View full text Add to dashboard Cite

In this study, diarrhea was induced in rabbits by feeding them antibiotic-free feed. The gut provides important defense against the barriers of the body, of which the duodenum is an important part to help digest food and absorb nutrients. However, the mechanisms underlying the roles of the gut microbiome and fecal metabolome in rabbit diarrhea caused by feeding an antibiotic-free diet have not been characterized. Recently, only a single study has been conducted to further characterize the antibiotic-free feed additives that caused diarrhea in weaned rabbits. The multi-omics techniques, including 16S rRNA sequencing, transcriptome sequencing, and LC-MS analysis, were combined to analyze the gut microbial compositions and functions. They also determined the fecal metabolomic profiles of diarrhea in rabbits caused by feeding antibiotic-free feed. The results showed that the liver, duodenal, and sacculus rotundus tissues of diarrhea rabbits were diseased, the composition of intestinal microbes was significantly changed, the diversity of intestinal microbes was decreased, and the distribution of intestinal microbe groups was changed. Functional analysis based on the cluster of GO and KEGG annotations suggested that two functional GO categories belonged to the metabolism cluster, and five KEGG pathways related to the metabolic pathways were significantly enriched in diarrhea rabbits. Moreover, real-time quantitative PCR (RT-qPCR) was used to verify the significant expression of genes related to diarrhea. Metabolomics profiling identified 432 significantly differently abundant metabolites in diarrhea rabbits, including amino acids and their derivatives. These amino acids were enriched in the tryptophan metabolic pathway. In addition, the functional correlation analysis showed that some altered gut microbiota families, such as Parasutterella, significantly correlated with alterations in fecal metabolites. Collectively, the results suggested that altered gut microbiota was associated with diarrhea caused by antibiotic-free feed additives in weaned rabbit pathogenesis.

show abstract

“…

25R)-spirost-5-en-3β-Ol (Figure 1), is a phytosteroidal compound extracted from the rhizome of dioscorea. (1) Studies have shown that diosgenin has antieffects on blood lipid, (2) diabetes, (3) thrombus, (4) myocardial ischemia, (5) rheumatoid arthritis, (6) inflammation, (7) allergy, (8) immune regulation. (9) Concurrently, it also has a resisting effect on neoplasm, nephropathy, (10) encephalomyelitis, (11) osteoporosis, (12) Parkinson's disease, (13) Alzheimer's disease, (14) and plays a beneficial role in pulmonary hypertension.

…”

mentioning

confidence: 99%

Anticancer Activity of Diosgenin and Its Molecular Mechanism

Ren

Wang

Zhang

et al. 2023

Chin. J. Integr. Med.

View full text Add to dashboard Cite

Diosgenin, a steroidal sapogenin, obtained from Trigonella foenum-graecum, Dioscorea , and Rhizoma polgonati , has shown high potential and interest in the treatment of various cancers such as oral squamous cell carcinoma, laryngeal cancer, esophageal cancer, liver cancer, gastric cancer, lung cancer, cervical cancer, prostate cancer, glioma, and leukemia. This article aims to provide an overview of the in vivo, in vitro , and clinical studies reporting the diosgenin’s anticancer effects. Preclinical studies have shown promising effects of diosgenin on inhibiting tumor cell proliferation and growth, promoting apoptosis, inducing differentiation and autophagy, inhibiting tumor cell metastasis and invasion, blocking cell cycle, regulating immunity and improving gut microbiome. Clinical investigations have revealed clinical dosage and safety property of diosgenin. Furthermore, in order to improve the biological activity and bioavailability of diosgenin, this review focuses on the development of diosgenin nano drug carriers, combined drugs and the diosgenin derivatives. However, further designed trials are needed to unravel the diosgenin’s deficiencies in clinical application. Electronic Supplementary Material Supplementary material is available in the online version of this article at 10.1007/s11655-023-3693-1.

show abstract

Anti-Allergic Diarrhea Effect of Diosgenin Occurs via Improving Gut Dysbiosis in a Murine Model of Food Allergy

Cited by 5 publications

References 44 publications

Resveratrol Treatment Prevents Increase of Mast Cells in Both Murine OVA Enteritis and IL-10−/− Colitis

Resveratrol Treatment Prevents Increase of Mast Cells in Both Murine OVA Enteritis and IL-10−/− Colitis

Microbiome, Transcriptome, and Metabolomic Analyses Revealed the Mechanism of Immune Response to Diarrhea in Rabbits Fed Antibiotic-Free Diets

Anticancer Activity of Diosgenin and Its Molecular Mechanism

Contact Info

Product

Resources

About