Anti‐allergic effects of <i>Lactobacillus crispatus</i> KT‐11 strain on ovalbumin‐sensitized BALB/c mice

Tobita, Keisuke; Yanaka, Hiroyuki; Otani, Hajime

doi:10.1111/j.1740-0929.2010.00795.x

Cited by 22 publications

(22 citation statements)

References 30 publications

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“…Lactobacillus crispatus is an obligately homofermentative, catalase-negative, Gram-positive bacterium [1]. Numerous studies have shown the considerable probiotic potential of L. crispatus, such as modulating the host's immune system [2], reducing allergic symptoms in mice [3], down-regulating the expression of cancer genes, and inhibiting the proliferation of MDA-MB-231 breast cancer cell lines [4]. In addition, as one of the most detected Lactobacillus species in the healthy female vagina [5], L. crispatus can inhibit vaginal pathogenic microorganisms, and maintain the health of the female reproductive system [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Lactobacillus crispatus from the Gut and Vagina Reveals Genetic Diversity and Lifestyle Adaptation

Zhang

Ross

et al. 2020

Genes

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Lactobacillus crispatus colonizes the human feces, human vagina, and the crops and ceca of chicken. To explore the genetic characteristics and evolutionary relationships of L. crispatus isolated from different niches, we selected 37 strains isolated from the human vagina (n = 17), human feces (n = 11), and chicken feces (n = 9), and used comparative genomics to explore the genetic information of L. crispatus from the feces and vagina. No significant difference was found in the three sources of genomic features such as genome size, GC content, and number of protein coding sequences (CDS). However, in a phylogenetic tree constructed based on core genes, vagina-derived L. crispatus and feces-derived strains were each clustered separately. Therefore, the niche exerted an important impact on the evolution of L. crispatus. According to gene annotation, the L. crispatus derived from the vagina possessed a high abundance of genes related to acid tolerance, redox reactions, pullulanase, and carbohydrate-binding modules (CBMs). These genes helped L. crispatus to better adapt to the acidic environment of the vagina and obtain more nutrients, maintaining its dominance in the vagina in competition with other strains. In feces-derived bacteria, more genes encoding CRISPR/Cas system, glycoside hydrolases (GHs) family, and tetracycline/lincomycin resistance genes were found to adapt to the complex intestinal environment. This study highlights the evolutionary relationship of L. crispatus strains isolated from the vagina and feces, and the adaptation of L. crispatus to the host environment.

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Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Lactobacillus crispatus from the Gut and Vagina Reveals Genetic Diversity and Lifestyle Adaptation

Zhang

Ross

et al. 2020

Genes

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“…In a previous paper, we found that Lactobacillus crispatus KT-11 (KT-11) reduced some allergic symptoms via a decrease in antigen-specific IgE levels in NC/Nga mice, and the reduction was concluded to be due to an adjustment in the Th1/Th2 balance via Tolllike receptor (TLR) 2, nucleotide-binding oligomerization domain (NOD) 1, and/or NOD2 ( 6 ). Moreover, we observed that KT-11 suppressed the acquired allergic responses via not only the adjustment in Th1/Th2 balance but also the decrease in spleen mast cell numbers and antigen-presenting cell numbers in ovalbuminimmunized BALB/c mice ( 7 ). However, the effect of KT-11 on immune systems of normal mice is not known.…”

mentioning

confidence: 67%

Lactobacillus crispatus KT-11 Enhances Intestinal Immune Functions in C3H/HeN Mice

Tobita

Yanaka²,

Otani

2010

J Nutr Sci Vitaminol

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SummaryWe investigated the effect of Lactobacillus crispatus KT-11 (KT-11) on intestinal immune systems in C3H/HeN mice. The level of intestinal total immunoglobulin (Ig) A was significantly higher in mice given KT-11 than in mice not given KT-11. Gene expression relating to antibody production and innate immune response increased more than 2-fold in the former compared with the later. Moreover, the number of IL-6 ϩ CD11bϩ cells was significantly higher in Peyer's patch cells cultured with KT-11 than in those cultured without KT-11, although the number of CD4 ϩ cells and the cell ratio of CD4 ϩ /CD8 ϩ were remarkably lower in the culture with KT-11. These results indicate that KT-11 enhances intestinal IgA production and innate immune response in C3H/HeN mice.

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“…The proportion of several bacterial genera ( Lactobacillus , Alistipes , Roseburia , Adlercreutzia and Clostridium ) known to have immunomodulatory properties was significantly different between farm and AF mice. Immunomodulatory properties have in particular been shown for Lactobacillus Crisptatus , and for Clostridia colonization in relation to IL‐22 up‐regulation in lamina propria lymphocytes . In contrast to AF mice whose microbiome had higher diversity at 4 weeks than at 20 weeks of age ( P = 0.0079), the Shannon diversity index of farm mice significantly ( P = 0.032) increased during this time interval from 2.89 (range 1.8–3.18) to 3.97 (range 3.84–4.16).…”

Section: Discussionmentioning

confidence: 99%

The farming environment protects mice from allergen‐induced skin contact hypersensitivity

Frossard

Lazarević

Gaïa

et al. 2017

Clin Experimental Allergy

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SummaryBackground Being born and raised in a farm provides a long-lasting protection for allergies. The microbial environment provided by farm animals is crucial to induce this protective effect, although underlying immune mechanisms remain elusive. Objective To establish a mouse model of global exposure to the farming environment and to study immunologic changes linked to protection of allergy. Methods Mice colonies were bred in parallel in a farm cowshed and the university animal facility (AF). Mice from both locations were subjected to a skin contact allergy model. Peripheral blood cells and cell cytokine production were assessed in both populations. In addition, the gut microbiome at various ages was characterized. Results Mice born in the farm were less prone to develop allergy than mice bred in the AF. Mice transfers between the AF and the farm showed a better protection when mice were moved to the farm early in life. As compared to AF-bred mice, farm mice displayed early immune activation with higher CD4 + T cell population, in particular CD4 + CD25 + FoxP3 À (activated cells). The cytokine profile of mice from the farm was skewed towards an IL-17 and IL-22 secreting cell profile accompanied by increased IL-10 secretion. These differences were mostly seen within a specific age window between birth and 8 weeks of age. Microbiome analysis showed differences between 4 and 20 weeks old mice and between farm and AF mice with an increased number of Murine mastadenovirus B in young farm mice exclusively. Conclusion The farming environment provides a strong, allergy protective IL-22 stimulus and generates activated CD4+ T cells. Exposure to the farm environment early in their life may also provide a better protection for contact skin allergy.Whether a viral trigger might decisively influence protection for allergies remains to be determined.

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Anti‐allergic effects of Lactobacillus crispatus KT‐11 strain on ovalbumin‐sensitized BALB/c mice

Cited by 22 publications

References 30 publications

Comparative Genomics of Lactobacillus crispatus from the Gut and Vagina Reveals Genetic Diversity and Lifestyle Adaptation

Comparative Genomics of Lactobacillus crispatus from the Gut and Vagina Reveals Genetic Diversity and Lifestyle Adaptation

Lactobacillus crispatus KT-11 Enhances Intestinal Immune Functions in C3H/HeN Mice

The farming environment protects mice from allergen‐induced skin contact hypersensitivity

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