Anti‐allergic effects of total bakkenolides from Petasites tricholobus in ovalbumin‐sensitized rats

Zhang, Fu-Jin; Wang, Qin; Wang, Yan; Guo, Meili

doi:10.1002/ptr.3237

Cited by 17 publications

(14 citation statements)

References 34 publications

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“…P. japonicus has been used to treat various diseases, including headaches, chronic cough, fever, and asthma. A bioassay study has demonstrated that P. japonicus extracts and compounds have antioxidant, anti-inflammatory, and antitumor biological effects (22). P. japonicus bakkenolide B has been previously demonstrated by our group to exhibit both antiallergenic and anti-inflammatory effects (23).…”

Section: Petasites Japonicus Bakkenolide B Inhibits Lipopolysaccharidmentioning

confidence: 93%

Petasites japonicus bakkenolide B inhibits lipopolysaccharide‑induced pro‑inflammatory cytokines via AMPK/Nrf2 induction in microglia

et al. 2017

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Abnormal neuroinflammatory responses have diverse roles in neuronal death, oxidative stress and neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Microglia regulate these responses via molecular signaling cascades that involve inflammatory cytokines and complement proteins. Bakkenolide B from Petasites japonicus exhibits significant anti‑inflammatory and anti‑allergic bioactivities. The present study investigated the anti‑neuroinflammatory effects and underlying molecular mechanisms of bakkenolide B on the lipopolysaccharide (LPS)‑mediated neuroinflammatory response in microglia. The results indicated that bakkenolide B pretreatment significantly reduced microglial production of interleukin (IL)‑1β, IL‑6, IL‑12, and tumor necrosis factor (TNF)‑α. Furthermore, this effect was associated with reduced production of reactive oxygen species. The role of bakkenolide B was then evaluated in the upregulation of nuclear factor erythroid 2‑related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathways. The results suggested that bakkenolide B significantly upregulated Nrf2/ARE pathway‑related downstream factors, such as NADPH dehydrogenase quinone‑1 (NQO‑1) and heme oxygenase‑1 (HO‑1). Silencing of Nrf2, HO‑1 and NQO‑1 diminished the anti‑neuroinflammatory properties of bakkenolide B. AMP‑activated protein kinase (AMPK) activates the Nrf2/ARE signaling pathway, and the results of the present study demonstrated that bakkenolide B increased AMPK phosphorylation in microglia. In addition, an AMPK inhibitor abolished the bakkenolide B‑induced increase in nuclear Nrf2, NQO‑1 and HO‑1 protein expression. Finally, an AMPK inhibitor diminished the bakkenolide B‑mediated inhibition of LPS‑stimulated TNF‑α production. Taken together, the present results demonstrate that bakkenolide B may be an effective and therapeutically relevant AMPK/Nrf2 pathway activator for suppressing abnormal neuro-inflammation in neurodegenerative diseases.

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Section: Petasites Japonicus Bakkenolide B Inhibits Lipopolysaccharidmentioning

confidence: 93%

Petasites japonicus bakkenolide B inhibits lipopolysaccharide‑induced pro‑inflammatory cytokines via AMPK/Nrf2 induction in microglia

et al. 2017

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Section: Introductionmentioning

confidence: 97%

“…Moreover, some active extracts rich in bakkenolides also showed interesting pharmacological activities. A bakkenolide fraction with bakkenolide D, B, IIIa and Iva as the major components has shown beneficial effects on allergic rhinitis in ovalbuminsensitized Wistar rats (Zhang et al, 2011). The methanol extracts of P. japonicus have been reported to protect from brain damage (Sok et al, 2006), suppress preadipocyte differentiation, reduce visceral fat accumulation (Watanabe et al, 2010) and inhibit ovalbumin-induced airway inflammation in rats (Lee et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

LC‐MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies

Dai

Pei

Tang

et al. 2013

Biomedical Chromatography

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A sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for determination of bakkenolide D (BD), which was further applied to assess the pharmacokinetics of BD. In the LC-MS/MS method, the multiple reaction monitoring mode was used and columbianadin was chosen as internal standard. The method was validated over the range of 1-800 ng/mL with a determination coefficient >0.999. The lower limit of quantification was 1 ng/mL in plasma. The intra- and inter-day accuracies for BD were 91-113 and 100-104%, respectively, and the inter-day precision was <15%. After a single oral dose of 10 mg/kg of BD, the mean peak plasma concentration of BD was 10.1 ± 9.8 ng/mL at 2 h. The area under the plasma concentration-time curve (AUC0-24 h ) was 72.1 ± 8.59 h ng/mL, and the elimination half-life (T1/2 ) was 11.8 ± 1.9 h. In case of intravenous administration of BD at a dosage of 1 mg/kg, the AUC0-24 h was 281 ± 98.4 h⋅ng/mL, and the T1/2 was 8.79 ± 0.63 h. Based on these results, the oral bioavailability of BD in rats at 10 mg/kg is 2.57%.

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“…Extracts from Petasites genus have been shown to have anti-allergic and anti-inflammatory effects ( Fiebich et al ., 2005 ; Lee et al ., 2011 ; Zhang et al ., 2011 ). Furthermore, lipophilic extracts of rhizomes of Petasites hybridus L., Asteraceae (the butterbur) have been used to treat asthma ( Danesch, 2004 ; Brattstrom et al ., 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of S-Petasin on Disease Models of Asthma and Peritonitis

Lee

Kang

Noh

et al. 2015

Biomolecules & Therapeutics

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To explore the anti-allergic and anti-inflammatory effects of extracts of Petasites genus, we studied the effects of s-petasin, a major sesquiterpene from Petasites formosanus (a butterbur species) on asthma and peritonitis models. In an ovalbumin-induced mouse asthma model, s-petasin significantly inhibited the accumulations of eosinophils, macrophages, and lymphocytes in bronchoalveolar fluids. S-petasin inhibited the antigen-induced degranulation of β-hexosamidase but did not inhibit intracellular Ca2+ increase in RBL-2H3 mast cells. S-petasin inhibited the LPS induction of iNOS at the RNA and protein levels in mouse peritoneal macrophages. Furthermore, s-petasin inhibited the production of NO (the product of iNOS) in a concentration-dependent manner in the macrophages. Furthermore, in an LPS-induced mouse model of peritonitis, s-petasin significantly inhibited the accumulation of polymorpho nuclear and mononuclear leukocytes in peritoneal cavity. This study shows that s-petasin in Petasites genus has therapeutic effects on allergic and inflammatory diseases, such as, asthma and peritonitis through degranulation inhibition in mast cells, suppression of iNOS induction and production of NO in macrophages, and suppression of inflammatory cell accumulation.

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Anti‐allergic effects of total bakkenolides from Petasites tricholobus in ovalbumin‐sensitized rats

Cited by 17 publications

References 34 publications

Petasites japonicus bakkenolide B inhibits lipopolysaccharide‑induced pro‑inflammatory cytokines via AMPK/Nrf2 induction in microglia

Petasites japonicus bakkenolide B inhibits lipopolysaccharide‑induced pro‑inflammatory cytokines via AMPK/Nrf2 induction in microglia

LC‐MS/MS determination of bakkenolide D in rats plasma and its application in pharmacokinetic studies

Therapeutic Effects of S-Petasin on Disease Models of Asthma and Peritonitis

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