Anti-allergic function of the cell wall (DeinoWall) from Deinococcus radiodurans

Chen, Fengjia; Ji, Hyun Jung; Choi, Jong‐il; Han, Seung Hyun; Lim, Sangyong; Seo, Ho Seong; Ahn, Kyu Jeung

doi:10.1016/j.molimm.2022.09.004

Cited by 4 publications

(5 citation statements)

References 41 publications

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“…The antioxidant capability of DNX, which exceeds that of lycopene, β-carotene, and zeaxanthin, becomes especially significant in comprehending the cellular resilience of D. radiodurans when exposed to oxidative stress. Moreover, recent findings hint at the potential role of D. radiodurans, particularly its cell wall component known as DeinoWall, in modulating immunity and possibly mitigating allergic inflammatory responses, although the exact mechanisms remain to be elucidated [11]. Given this backdrop, the focus of our ongoing investigation revolves around DNX, a key constituent of DeinoWall, and its protective effects on human skin cells exposed to ultraviolet radiation.…”

Section: Discussionmentioning

confidence: 99%

“…The study further demonstrated that DeinoWall promotes the maturation of dendritic cells, favoring Th1-biased immunity, which may regulate allergic reactions. Overall, DeinoWall might offer a potential therapeutic avenue for allergic diseases [11]. However, the role of deinoxanthin and its mechanisms in UV radiation-caused skin damage is still not defined.…”

Section: Introductionmentioning

confidence: 99%

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Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Kuzucu

2023

CIMB

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This research delved into the protective capacities of deinoxanthin, a carotenoid present in Deinococcus radiodurans, against UVA- and UVB-mediated skin damage using human fibroblast foreskin cells (HFF-1). Using the MTT assay, HFF-1 cells treated with 10 µM DNX displayed 20% and 31.7% higher viability than the positive (Vitamin C-treated) and negative (DNX-untreated) control groups, respectively, upon 100 mJ/cm2 UVB exposure. At 24 J/cm2 UVA, 20 µM DNX-treated cells showed 80.6% viability, exceeding the positive and negative control groups by 28.6% and 33.6%, respectively. Flow cytometry analysis revealed that cells treated with DNX and exposed to 24 J/cm2 UVA exhibited a 69.32% reduction in apoptotic processes compared to untreated cells. Similarly, when exposed to 100 mJ/cm2 UVB, DNX-treated cells demonstrated a 72.35% decrease in apoptotic processes relative to their untreated counterparts. DNX also displayed dose-dependent inhibition on tyrosinase activity. The study emphasized DNX’s antioxidative capacity, evident in its modulation of superoxide dismutase activity and measurements of Malondialdehyde and intracellular reactive oxygen species levels. DNX-treated cells exhibited higher hydroxyproline levels, suggesting healthier collagen production. Additionally, the wound-healing assay method confirmed an accelerated healing rate in DNX-treated cells. Conclusively, DNX offers significant protection against UV-induced skin damage, emphasizing its potential for skincare and therapeutics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Kuzucu

2023

CIMB

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“…The severity of atopic dermatitis-like symptoms was evaluated every week after ESS treatment in BALB/c mice whose atopic dermatitis-like symptoms were induced by DNCB treatment. It was based on four pathological signs including erythema/hemorrhage, edema, excoriation/erosion, and scaling/dryness 36 – 38 . The scoring criteria were 0 (absent), 1 (mild), 2 (moderate), and 3 (severe) points according to severity, and the total clinical score was determined by the sum of all individual scores.…”

Section: Methodsmentioning

confidence: 99%

“…Four days later, after confirming the induction of atopic dermatitis-like symptoms, 200-400 μL of ESS dissolved in solution (Propylene glycol: ethanol = 7:3) and 200 μL of DNCB (0.5-0.25%) solution were applied daily for 4 weeks to prevent natural healing (Fig. 1a) [34][35][36] . The experiment groups consisted of non-induced atopic dermatitis-like symptoms group (CON, n = 7), DNCBinduced atopic dermatitis-like symptoms group (DNCB, n = 7), DNCB + ESS 500 ppm group (ESS 500 ppm, n = 6), DNCB + ESS 1000 ppm group (ESS 1000 ppm, n = 6), DNCB + ESS 2000 ppm group (ESS 2000 ppm, n = 7), DNCB + Dexamethasone 2000 ppm group (DEX 2000 ppm, positive control, n = 5).…”

Section: Dncb-induced Balb/c Mouse Atopic Dermatitis Modelmentioning

confidence: 99%

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The beneficial effects of ethanolic extract of Sargassum serratifolium in DNCB-induced mouse model of atopic dermatitis

Kim,

Ryu,

Jeong

et al. 2024

Sci Rep

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Atopic dermatitis is a chronic complex inflammatory skin disorder that requires sustainable treatment methods due to the limited efficacy of conventional therapies. Sargassum serratifolium, an algal species with diverse bioactive substances, is investigated in this study for its potential benefits as a therapeutic agent for atopic dermatitis. RNA sequencing of LPS-stimulated macrophages treated with ethanolic extract of Sargassum serratifolium (ESS) revealed its ability to inhibit a broad range of inflammation-related signaling, which was proven in RAW 264.7 and HaCaT cells. In DNCB-induced BALB/c or HR-1 mice, ESS treatment improved symptoms of atopic dermatitis within the skin, along with histological improvements such as reduced epidermal thickness and infiltration of mast cells. ESS showed a tendency to improve serum IgE levels and inflammation-related cytokine changes, while also improving the mRNA expression levels of Chi3l3, Ccr1, and Fcεr1a genes in the skin. Additionally, ESS compounds (sargachromanol (SCM), sargaquinoic acid (SQA), and sargahydroquinoic acid (SHQA)) mitigated inflammatory responses in LPS-treated RAW264.7 macrophages. In summary, ESS has an anti-inflammatory effect and improves atopic dermatitis, ESS may be applied as a therapeutics for atopic dermatitis.

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A Novel Deinococcus Antioxidant Peptide Mitigates Oxidative Stress in Irradiated CHO-K1 Cells

Lim,

Song,

Park

et al. 2024

Microorganisms

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Reactive oxygen species (ROS), byproducts of cellular metabolism and environmental factors, are linked to diseases like cancer and aging. Antioxidant peptides (AOPs) have emerged as effective countermeasures against ROS-induced damage. The Deinococcus genus is well known for its extraordinary resilience to ionizing radiation (IR) and possesses complex antioxidant systems designed to neutralize ROS generated by IR. In this study, we developed four peptides, each containing 9 to 11 amino acids, from the leaderless mRNA (lmRNA) sequences of D. deserti. Lacking a 5′ untranslated region, lmRNAs directly initiate protein synthesis, potentially encoding small peptides such as AOPs. Of the four peptides, Ddes-P3 was found to exhibit significant antioxidant capabilities in vitro, effectively scavenging ABTS radicals. Ddes-P3 provided considerable defense against IR-induced oxidative stress in CHO-K1 cells, demonstrating a notable reduction in ROS production and lipid peroxidation. The peptide’s potential was highlighted by its ability to enhance cell survival and maintain mitochondrial membrane potential under irradiative stress, suggesting its utility as a nontoxic and effective radioprotector in mitigating radiation-induced cellular damage. This study explores the potential role of lmRNA in synthesizing AOPs within Deinococcus. Identifying lmRNAs that encode AOPs could deepen our understanding of their cellular resistance to oxidative stress and pave the way for creating innovative biotechnological and therapeutic AOPs.

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Anti-allergic function of the cell wall (DeinoWall) from Deinococcus radiodurans

Cited by 4 publications

References 41 publications

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

The beneficial effects of ethanolic extract of Sargassum serratifolium in DNCB-induced mouse model of atopic dermatitis

A Novel Deinococcus Antioxidant Peptide Mitigates Oxidative Stress in Irradiated CHO-K1 Cells

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