Anti–anaphylactic and anti–inflammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir

Abstract

In the present study, anti-anaphylactic, anti-inflammatory and membrane stabilizing properties of a lupine alkaloid plumerianine (compound 1), isolated from the root bark of Plumeria acutifolia Poir were investigated in animal models. The anti-anaphylactic activity of compound 1 (10, 25 and 50 mg/Kg) was studied by using models such as passive cutaneous anaphylaxis, passive paw anaphylaxis and also investigated for its anti-inflammatory activity against the carrageenin induced paw edema and cotton pellet granuloma in albino rats. A dose-dependent beneficial effect was observed on the leakage of Evans Blue dye in skin challenged with antigen and on paw anaphylaxis induced by antiserum. The compound 1 also exhibited a significant (p < 0.01) inhibition of rat paw edema and granuloma tissue formation, including significant protection of RBC against the hemolytic effect of hypotonic solution, an indication of membrane-stabilizing activity. Anti-anaphylactic activity of compound 1 may be possibly due to the inhibition of releasing various inflammatory mediators. Anti-inflammatory activity of compound 1 may be related to the inhibition of the early phase and late phase of inflammatory events.

Therapeutic effect of an alkaloid-rich fraction of Abrus precatorius seed methanol extract on paracetamol-induced liver damage in rats

“…sign of hepatotoxicity, however, the significant (p< 0.05) increase in the levels of these enzymes were not observed in the groups treated with alkaloid-rich fraction as shown in Table 1 indicating that the fraction was able to inhibit destruction of the cell membrane by toxic metabolite (NAPQI) of paracetamol and preventing leakage of the hepatocellular enzymes into the system. This suggest that alkaloids have membrane stabilization effect which is in tandem with the works of Vijayalakshmi et al (2011) where the anti-anaphylactic and antiinflammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir were analyzed.…”

<i>In vitro</i> anticancer activity and cytotoxicity of some papaver alkaloids on cancer and normal cell lines

“…Alkaloids are endowed with diverse biological activities, being already used in therapy as pharmacological tools. Among the reported biological effects (Awaad et al 2007), antidepressant (Nesterova et al 2011), antimicrobial (Karou et al 2006), antiemetic (Bulbul et al 2013), and anti-inflammatory (Vijayalakshmi et al 2011) properties. Nonetheless, there are also reports of toxic effects to humans; thus, the use of different experimental models to understand the exact mechanism of the molecules under study is necessary, in order to have the real knowledge of their effect (Nascimento et al 2015).…”

A review of the use of pteridophytes for treating human ailments

“…The free radical scavenging activity of plant products is primarily due to various bioactive compounds like phenolics, flavonoids, phenolic diterpenes, and tannins (Lee et al, 2004). Currently, steroidal and nonsteroidal anti-inflammatory drugs (NSAIDs) used to treat inflammatory disease frequently produce severely harmful effects (Vonkeman and van de Laar, 2010;Vijayalakshmi et al, 2011). Thus, there is abundant motivation to find new drugs with antiinflammatory properties and fewer side effects, especially from plants used in folk medicine.…”