Anti-angiogenic effect of siphonaxanthin from green alga, Codium fragile

Abstract

Since anti-angiogenic therapy has becoming a promising approach in the prevention of cancer and related diseases, the present study was aimed to examine the anti-angiogenic effect of siphonaxanthin from green alga (Codium fragile) in cell culture model systems and ex vivo approaches using human umbilical vein endothelial cells (HUVECs) and rat aortic ring, respectively. Siphonaxanthin significantly suppressed HUVEC proliferation (p<0.05) at the concentration of 2.5 μM (50% as compared with control) and above, while the effect on chemotaxis was not significant. Siphonaxanthin exhibited strong inhibitory effect on HUVEC tube formation. It suppressed the formation of tube length by 44% at the concentration of 10 μM, while no tube formation was observed at 25 μM, suggesting that it could be due to the suppression of angiogenic mediators. The ex vivo angiogenesis assay exhibited reduced microvessel outgrowth in a dose dependent manner and the reduction was significant at more than 2.5 μM. Our results imply a new insight on the novel function of siphonaxanthin in preventing angiogenesis related diseases.

The Effect of Alcoholic Extract of Persian Gulf Chiton (lamyi) Shell on Angiogenesis in Chick Chorioallantoic Membrane

“…Their results showed that Sargassum seaweed extract can reduce the length and number of blood vessels in the CAM model [12]. Also Ganesan et al showed that the siphonaxanthin is derived from green algae can inhibit angiogenesis in ex vivo and in vitro [13].…”

Piceatannol inhibits MMP-9-dependent invasion of tumor necrosis factor-α-stimulated DU145 cells by suppressing the Akt-mediated nuclear factor-κB pathway

“…The generation and analysis of transgenic and knockout mice for MMPs have confirmed that MMPs play key roles in the process of carcinogenesis and invasion (21). One of these, MMP-9, is regarded as a critical molecule in tumor progression and invasion (22). Therefore, inhibition of MMP-9 appears to be an ideal strategy to control tumor growth, invasion and metastasis.…”

Cytotoxic and Anti-angiogenic Properties of Minor 3,4-<i>seco</i>-Cycloartanes from <i>Gardenia sootepensis</i> Exudate

“…21,22) HUVECs were plated onto 96-well plates (1.5×10 3 cells/well) and incubated for 24 h at 37°C in 5% CO 2 . The medium was removed and replaced with fresh HuMedia EG2 containing various doses of compound or vehicle (DMSO) and incubated for 72 h under the same condition.…”

“…21,22) Solid gel was prepared on a 96-well tissue culture plate according to the manufacture's instruction. HUVECs (1×10 5 cells/mL) in HuMedia EG2 medium containing various doses of compound or vehicle (DMSO) (100 µL) were seeded onto the surface on BD Matrigel.…”

“…21,22) A microporous membrane (8 µm) of 24-well cell culture inserts was coated with 0.1% gelatin. A HUVEC suspension in Medium 199 with 0.1% bovine serum albumin (2.5×10 5 cells/mL) was seeded in each chamber (400 µL).…”