Anti-angiogenic effects of a nutrient mixture on human umbilical vein endothelial cells

Roomi, M. Waheed; Ivanov, Vadim; Kalinovsky, Tatiana; Niedzwiecki, Aleksandra; Rath, Matthias

doi:10.3892/or.14.6.1399

Cited by 30 publications

(52 citation statements)

References 8 publications

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“…Previous studies have reported differential effects of antiangiogenic factors that preserved endothelial proliferation but inhibited tube formation capacity. 29,30 The results of this study suggested that LDL apheresis increased activated eNOS protein expression and proliferative activity without a promotion of angiogenic properties and that the activating effects of LDL apheresis and the angiogenic cytokines VEGF and HGF occur through different mechanisms. Additional studies are necessary to address the molecular mechanisms of this differential activating effect of LDL apheresis on endothelial cells, including identification of the responsible factor(s) involved.…”

Section: Discussionmentioning

confidence: 93%

Sustained Inhibition of Oxidized Low-Density Lipoprotein Is Involved in the Long-Term Therapeutic Effects of Apheresis in Dialysis Patients

Tsurumi‐Ikeya

Tamura

Azuma

et al. 2010

ATVB

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Objective-Low-density lipoprotein (LDL) apheresis is a potential therapy for conventional therapy-resistant peripheral artery disease. In the present study, we examined the chronic effects of LDL apheresis on clinical parameters in vivo and endothelial cell functions in vitro in hemodialysis patients who had the complication of peripheral artery disease. Methods and Results-Twenty-five patients were enrolled, and the responses of 19 patients to LDL apheresis were analyzed. Patients were classified into 2 groups according to change in ankle-brachial pressure index (ABI) after treatment: patients with improved ABI (responders, nϭ10) and patients with worsened ABI (nonresponders, nϭ9). In the responders, apheresis resulted in a long-term reduction of circulating levels of oxidized LDL, C-reactive protein, and fibrinogen. In human umbilical vein endothelial cells (HUVECs), the serum from the responders increased expression of activated endothelial nitric oxide synthase protein and proliferative activity. Key Words: atherosclerosis Ⅲ endothelium Ⅲ nitric oxide synthase Ⅲ oxidized lipids Ⅲ peripheral arterial disease Ⅲ lipoproteins Ⅲ oxidative stress C ardiovascular disease is the primary cause of death in patients with end-stage renal disease. Patients on dialysis are reported to have a 10 -20-fold greater risk of cardiovascular disease-associated mortality than the general population after stratification for age, gender, race, and the presence or absence of diabetes. Patients undergoing dialysis have many of the risk factors for atherosclerosis, such as hypertension, dyslipidemia, and disturbed calcium-phosphate metabolism, and, indeed, they commonly experience severe atherosclerosis, including peripheral artery disease (PAD). Low-density lipoprotein (LDL) apheresis is a potentially useful treatment for conventional therapy-resistant hypercholesterolemic patients with coronary artery disease and PAD. 1,2 Previously, it was shown that a single LDL apheresis session enhanced the peripheral microcirculation, probably by increasing the production of nitric oxide (NO) and bradykinin, 3 reducing blood viscosity and adhesion molecules, 4 and inducing endothelium-dependent vasodilatation. 5 However, the precise molecular mechanism of the long-term effects of LDL apheresis on the improvement of the peripheral circulation remains unclear and warrants further investigation.We undertook the present study to investigate the shortand long-term effects of LDL apheresis on clinical and laboratory parameters in vivo and vascular endothelial cell function in vitro, in hemodialysis patients with PAD, and to identify factors related to the improvement of the peripheral circulation by LDL apheresis. Methods Patients and Study DesignThe study protocol was approved by the Human Ethics Committee of Yokohama City University Hospital. A total of 25 consecutive hemodialysis patients with leg impairments and ankle-

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Section: Discussionmentioning

confidence: 93%

Sustained Inhibition of Oxidized Low-Density Lipoprotein Is Involved in the Long-Term Therapeutic Effects of Apheresis in Dialysis Patients

Tsurumi‐Ikeya

Tamura

Azuma

et al. 2010

ATVB

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“…53) Glycine, which is an anti-inflammatory agent has similar properties. 54,55) Additionally, the NMR analysis confirmed the presence of phenolics that were previously identified by mass spectrometric analysis.…”

Section: )mentioning

confidence: 99%

NMR Metabolomic Analysis of Fecal Water from Subjects on a Vegetarian Diet

Pettersson

Karlsson

Choi

et al. 2008

Biological & Pharmaceutical Bulletin

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Plant based food contains a multiplicity of bioactive phytochemicals. These compounds are believed to contribute to the cancer protective effect of a diet high in fruit and vegetables.1,2) One group of phytochemicals is the cyclooxygenase-2 (COX-2) inhibitors, which potentially can act as preventive agents against colon cancer. Such compounds and their derivatives may pass through the digestive system and reach the colon where they exert chemopreventive effects.The water fraction of feces (fecal water) is believed to be in direct contact with the colonocytes, and its composition appears to be important for a healthy environment in the colon.3) It is believed that the fecal water composition roughly reflects the food intake and that it contains a mixture of compounds that may prevent and promote cancer development. A favorable change in the composition of fecal water would consequently decrease the risk of developing colon cancer.4) Fecal water is nowadays a common tool used in human intervention studies. It is used to test cellular responses in vitro in cell systems. [5][6][7][8] Such studies have shown that fecal water can modulate several cellular parameters such as genotoxicity, proliferation and apoptosis, all of which are relevant for tumorigenesis. [9][10][11] Other human intervention studies have indicated that it is possible to alter the pH and the composition of bile acids in fecal water with a change in the diet. 3,12) Another parameter that can be modulated by fecal water is the colonic COX-2 enzyme. We have recently shown that fecal waters from human volunteers inhibit COX-2 protein expression and prostaglandin E 2 (PGE 2 ) production in colonic cells. 13)COX-2 is the inducible form of two isoforms of cyclooxygenases, and it catalyzes the production of prostaglandins. This enzyme is not normally expressed in the colon, but it can be induced by growth factors, cytokines, tumor necrosis factor and lipopolysaccharides in situations of stress. [14][15][16] An over expression of COX-2 protein and an increased production of prostaglandins have been reported in patients with colon cancer, [17][18][19] and inhibition of the COX-2 enzyme has been a drug target over the past fifteen years. Today, there are several specific COX-2 inhibitors available on the market, but some of these synthetic drugs were recently reported to have severe side effects on the cardiovascular system. 20,21) To characterize a complex mixture of metabolites such as fecal water, a comprehensive metabolite profiling method is required. Metabolomic profiling of complex extracts is not an easy task to perform since they usually display a wide range of structural diversity. Of the possible analytical methods used in metabolomics, NMR provides a rapid, reproducible method well suited for identification of many metabolites and it has several advantages compared to other methods. It is not only the high information content of the resulting spectra for chemical structures but also the convenience in practical aspects such as high reproducibilit...

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“…In human cervical (HeLa and DoTc2-4510) and ovarian (SK-OV-3) cancer cell lines, Roomi et al [27] demonstrated that treatment with IL-1␤ significantly inhibited MMP-2 and MMP-9 activities, suggesting that IL-1␤ has anti-metastatic potential. IL-12 has been shown to exhibit anti-tumor and antimetastatic activities against B16F10 melanoma through activation of natural killer (NK) activity and enhancement of T cell production [28].…”

Section: Discussionmentioning

confidence: 97%

Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells

Yang

Chen

et al. 2015

International Journal of Biological Macromolecules

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Anti-angiogenic effects of a nutrient mixture on human umbilical vein endothelial cells

Cited by 30 publications

References 8 publications

Sustained Inhibition of Oxidized Low-Density Lipoprotein Is Involved in the Long-Term Therapeutic Effects of Apheresis in Dialysis Patients

Sustained Inhibition of Oxidized Low-Density Lipoprotein Is Involved in the Long-Term Therapeutic Effects of Apheresis in Dialysis Patients

NMR Metabolomic Analysis of Fecal Water from Subjects on a Vegetarian Diet

Anti-metastatic effects of antrodan, the Antrodia cinnamomea mycelia glycoprotein, in lung carcinoma cells

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