Anti-Anxiety Effect of (−)-Syringaresnol-4-O-β-d-apiofuranosyl-(1→2)-β-d-glucopyranoside from Albizzia julibrissin Durazz (Leguminosae)

Abstract

Albizzia julibrissin Durazz, a Chinese Medicine, is commonly used for its anti-anxiety effects. (−)-syringaresnol-4-O-β-d-apiofuranosyl-(1→2)-β-d-glucopyranoside (SAG) is the main ingredient of Albizzia julibrissin Durazz. The present study investigated the anxiolytic effect and potential mechanisms on the HPA axis and monoaminergic systems of SAG on acute restraint-stressed rats. The anxiolytic effect of SAG was examined through an open field test and an elevated plus maze test. The concentration of CRF, ACTH, and CORT in plasma was examined by an enzyme-linked immune sorbent assay (ELISA) kit while neurotransmitters in the cerebral cortex and hippocampus of the brain were examined by High Performance Liquid Chromatography (HPLC). We show that repeated treatment with SAG (3.6 mg/kg, p.o.) significantly increased the number and time spent on the central entries in the open-field test when compared to the vehicle/stressed group. In the elevated plus maze test, 3.6 mg/kg SAG could increase the percentage of entries into and time spent on the open arms of the elevated plus maze. In addition, the concentration of CRF, ACTH, and CORT in plasma and neurotransmitters (NE, 5-HT, DA and their metabolites 5-HIAA, DOPAC, and HVA) in the cerebral cortex and hippocampus of the brain were decreased after SAG treatment, as compared to the repeated acute restraint-stressed rats. These results suggest that SAG is a potential anti-anxiety drug candidate.

Identification of Bioactive Chemical Markers in Zhi zhu xiang Improving Anxiety in Rat by Fingerprint-Efficacy Study

“…The OFT apparatus was a 180 cm diameter cylinder with 60 cm high walls. The center of the bottom of the apparatus had a 52 cm diameter section, as previously described [ 57 ]. The rats were placed into the same point of the field and were allowed to freely explore for 5 min.…”

“…The elevated plus maze was made of black plexiglas and consisted of two open arms (50.8 cm × 10.2 cm × 1.3 cm) and two closed arms (50.8 cm × 10.2 cm × 40.6 cm) that extended from a central platform (10.2 cm × 10.2 cm). The maze was elevated to a height of 72.4 cm above the floor [ 57 ]. After the OFT, rats were gently transported to the EPM room.…”

Core prescription pattern of Chinese herbal medicine for depressive disorders in Taiwan: A nationwide population-based study

“…The major constituents of Bai-He and He-Huan-Pi have been reported to have antidepressant effects. 61 , 62 Two recent in vivo studies have revealed that He-Huan-Pi had an anti-anxiety effect via the regulation of neurotransmitters 63 and the serotonergic nervous system. 64 Additionally, a study used a Chinese formula that consisted of He-Huan-Pi, Suan-Zao-Ren, Bai-Shao and Bai-Zi-Ren using a depressed mice model indicated that this formula was able to reduce the immobility time of depressed mice by inhibiting the monoamine oxidase enzyme system, as well as by increasing serotonin and noradrenaline levels.…”

Untitled

“…This antidepressant-like effect was reversed by treatment with a 5-HT 1A receptor antagonist but was not affected by treatment with 5-HT 1B or 5-HT 2A receptor antagonists; therefore, the plant extract seems to exert its antidepressant effects through 5-HT 1A receptors [48]. Several years later, Liu et al [49] determined that one of the active principles of this plant extract was the compound (−)-syringaresinol-4-O-β-D-apiofuranosyl-(1 → 2)-β-D-glucopyranoside (52, Fig. 6S, Supplementary Information), which increased the percentage of entries into and time spent on the open arms of the EPM while lowering the concentration of ACTH and corticosterone in plasma and decreasing the amount of neurotransmitters (DA, NA, 5-HT) and their metabolites in the cerebral cortex and hippocampus of treated rat brains.…”