Anti-apoptotic and cytoprotective effect ofEnicostemma littoraleagainst oxidative stress in Islets of Langerhans

Srivastava, Abhay; Bhatt, Nilesh; Patel, Tushar; Dadheech, Nidheesh; Singh, Anubha; Gupta, Sarita

doi:10.3109/13880209.2016.1141222

Cited by 10 publications

(7 citation statements)

References 53 publications

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“…In addition, we used ultra-thin sections and cell nucleus extraction methods to determine the concentration of chromatin. The results were similar to those of oxidative stress-induced apoptosis in the diabetic islet cells ( Srivastava et al, 2016 ) and suggested that the pulp cells of bitter pit undergo PCD. PCD is a physiological activity that regulates the body through active cell death ( Hautegem et al, 2015 ).…”

Section: Discussionsupporting

confidence: 82%

Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica

Qiu

Wang

et al. 2021

Front. Plant Sci.

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Apple bitter pit primarily occurs during fruit ripening and storage; however, its formation mechanism remains unclear. Although it is considered that Ca2+ deficiency causes metabolic disorders in apples, there have been few studies on the mechanism of the bitter pit from the perspective of cell structure. At the fruit ripening stage, the fruit with a bitter pit on the tree was taken as the research material. In this study, the microscopic observation revealed numerous amyloplasts in the pulp cells of apples affected with bitter pit, but not in the healthy pulp. Furthermore, the results of fluorescence staining and transmission electron microscopy (TEM) revealed that the bitter pit pulp cells undergo programmed cell death (PCD), their nuclear chromosomes condense, and amyloplast forms autophagy. The cytoplasmic Ca2+ concentration in the healthy fruits was lowest near the peduncle, followed by that in the calyx, whereas it was highest at the equator. In contrast, the cytoplasmic Ca2+ concentration in apple fruits showing bitter pit disorder was lowest near the peduncle and highest in the calyx. Moreover, the cytosolic Ca2+ concentration in the flesh cells of apples with the bitter pit was much lower than that in the healthy apple flesh cells; however, the concentration of Ca2+ in the vacuoles of fruits with the bitter pit was higher than that in the vacuoles of healthy fruits. In summary, bitter pit pulp cells contain a large number of amyloplasts, which disrupts the distribution of Ca2+ in the pulp cells and causes PCD. These two processes lead to an imbalance in cell metabolism and induce the formation of a bitter pit.

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Section: Discussionsupporting

confidence: 82%

Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica

Qiu

Wang

et al. 2021

Front. Plant Sci.

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“…The UV-vis absorption spectrum showed the maximum absorbance peak at 416 nm. In recent contributions, the synthesis of nanoparticles using bacteria including Pseudomonas deceptionensis, Weissella oryzae [70], Bacillus amyloliquefaciens, Bacillus licheniformis, and Rhodobacter sphaeroides [71][72][73] was reported. The extracellular synthesis of AgNPs was reported with the use of bacteria such as Acinetobacter calcoaceticus [74], Gluconobacter roseus [75], Klebsiella pneumonia [76], Salmonella typhimurium [77], Pseudomonas aeruginosa [78], Xanthomonas oryzae [79], and Rhodococcus sp.…”

Section: Synthesis Of Agnps Using Bacteriamentioning

confidence: 99%

Biogenic Synthesis of Silver Nanoparticles, Characterization and Their Applications—A Review

2021

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With the growing awareness for the need of sustainable environment, the importance of synthesizing and the application of green nanoparticles has gained special focus. Among various metal nanoparticles, silver nanoparticles (AgNPs) have gain significant attention. AgNPs are synthesized conventionally by physical and chemical methods using chemicals such as reducing agents, which are hazardous to environment due to their toxic properties, provoking a serious concern to create and develop environment friendly methods. Thus, biological alternatives are emerging to fill gaps, such as green syntheses that use biological molecules taken from plant sources in the form of extracts, which have shown to be superior to chemical and physical approaches. These biological molecules derived from plants are assembled in a highly regulated manner to make them suitable for metal nanoparticle synthesis. The current review outlines the wide plant diversity that may be used to prepare a rapid and single-step procedure with a green path over the traditional ones, as well as their antifungal activity.

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“…Also the extract complies for heavy metal and microbial count as per the regulatory guideline 35,36 . The methanolic extract was reported to reduce the oxidative stress, induce apoptosis of cells and prolong survival of the tumor bearing mice 37,38 . The present study suggests that the alcoholic extract might be responsible for the anticancer activity in HCC in a similar way as reported in the literature.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Anticancer Activity of E. littorale Extract on Hepatocellular Carcinoma

Zanwar,

Patel,

Mandhane

2024

JNR

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Enicostemma littorale Blume is a perennial herb of the Gentianaceae family. It has been noted that different parts of the plant have hepatoprotective, anti-ulcer, antioxidant, antibacterial, and hypoglycemic properties. The aim of the current study was to assess the anticancer potential of the plant in vitro as well as in vivo animal studies. First, we evaluated the cytotoxic effect of the extracts in HepG2 cells. The alcoholic extract exhibited concentration-dependent cytotoxicity. In HepG2 cells, the extract’s anticancer activity was observed with an IC50 value of 373 ± 3.0 µg/mL. Following in vitro studies, the in vivo anticancer effectiveness of the extract was evaluated using a xenograft model. Vehicle-treated mice showed a time-dependent increase in tumor volume. Mice treated with the extract showed a decrease in tumor growth as compared to vehicle treated group, indicating the anticancer activity of the extract in tumor xenografts study. On day 21, a marked reduction in tumor volume was noted. The findings of the study suggest that the alcoholic extract of E. littorale is effective against hepatocellular carcinoma. To fully understand the anticancer potential of E. littorale, further research with specific phytoconstituents is required.

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Anti-apoptotic and cytoprotective effect ofEnicostemma littoraleagainst oxidative stress in Islets of Langerhans

Cited by 10 publications

References 53 publications

Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica

Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica

Biogenic Synthesis of Silver Nanoparticles, Characterization and Their Applications—A Review

<i>In Vitro</i> and <i>In Vivo</i> Anticancer Activity of <i>E. littorale</i> Extract on Hepatocellular Carcinoma

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