Anti-arthritogenic and cardioprotective action of hesperidin and daidzein in collagen-induced rheumatoid arthritis

Abstract

Atherosclerosis has been linked to chronic inflammatory processes. Changes in the levels of lipoproteins, especially low-density lipoprotein or its variants, as well as inflammatory markers are risk factors for the atherosclerosis. In the present study, an experimental model of rheumatoid arthritis was developed by administrating collagen suspension intradermally in the tail region of Wistar albino rats. At the same time, a suspension of hesperidin (50 mg/kg body weight) and daidzein (20 mg/kg body weight) was orally administrated. The compounds were given in the morning and evening for 21 days. Levels of inflammatory markers in the homogenate of knee joints of experimental rats as well as plasma lipoproteins were investigated. The administration of hesperidin and daidzein caused significant (p < 0.001) decrease in articular elastase activity, TNF-α, and malondialdehyde levels. Further, arthritis scoring and histological findings supported the anti-inflammatory actions of the test compounds. Interestingly, the test compounds also lowered the plasma low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, and triglyceride but increased the level of high-density lipoprotein cholesterol. The test compounds thus ameliorated the risk factors of atherosclerosis. Furthermore, antioxidant roles of hesperidin as well as daidzein were evident from decrease in free radical load demonstrated as increase in total antioxidant level in plasma of arthritic animals treated with hesperidin and daidzein. In a separate in vitro experiment, enhanced free radical scavenging activity of hesperidin was demonstrated against 2,2-diphenyl-1-picrylhydrazyl and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid. The anti-inflammatory, hypolipidemic, and antioxidant actions of the naturally occurring test compounds, particularly hesperidin, seem to be quite effective against rheumatoid arthritis and atherosclerosis. Thus, their consumption may be helpful in prevention or at least delaying the onset of these diseases in susceptible individuals.

Targeting Foam Cell Formation in Atherosclerosis: Therapeutic Potential of Natural Products

“…In addition, in male middle-aged rats with HFD used to induce atherosclerosis, treatment with daidzein (30 mg/kg body weight) decreased the serum cholesterol and increased TG level (Sosi c-Jurjevi c et al, 2007). In another study using rheumatoid arthritis rat model, it was shown that intragastric administration of daidzein (20 mg/kg body weight) and hesperidin (50 mg/kg body weight) reduced plasma VLDL, LDL-C, and TG concentrations, while increasing HDL-C levels (Ahmad et al, 2016).…”

“…Decreased the serum cholesterol, increased triglyceride (TG) level in the male middleaged rats with HFD (Sosi c-Jurjevi c et al, 2007), reduced plasma VLDL, LDL-C, and TG concentrations, while increased the HDL-C levels in a rheumatoid arthritis rat model (Ahmad et al, 2016). Daidzein was reported to be an inhibitor of HMG-CoA reductase, ACAT1, and ACAT2 (Borradaile et al, 2002;Sung et al, 2004).…”

The Anti-RA Activities of a Couplet Medicinals, Gastrodia Elata and Radix Aconitic Lateralis preparata, Explored by Untargeted Metabolomics and Network Pharmacology.

“…Hence, appropriate intake of linoleic acid has effective anti-RA activities [14][15][16] . Daidzein and daidzin can ameliorate RA symptoms and decrease RA occurrence [17][18][19][20] . Our results demonstrated that the content of linoleic acid in TF11 was higher than that of TF32, while the daidzin content of TF32 was higher than either TF11 or TF23 (Table 1).…”

The dietary isoflavone daidzein mitigates oxidative stress, apoptosis, and inflammation in CDDP‐induced kidney injury in rats: Impact of the MAPK signaling pathway

“…DZ has been reported to have several health benefits . Studies have shown that DZ possesses antioxidant, anti‐inflammatory, antiapoptotic, anticancer, MAPK signaling pathway modulating, and neuroprotective properties. The nephroprotective effect of DZ in mice has recently been demonstrated using a 3‐day model of CIN.…”