Anti-Auxin Enhance Rosa Hybrida L. Micropropagation

Singh, Shashi Kant; Syamal, M.M.

doi:10.1023/a:1002768613872

Cited by 16 publications

(4 citation statements)

References 15 publications

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“…The beneficial effects of adding exogenous antiauxin to the medium are likely a result of altering the balance of auxins and cytokinins, thereby preventing the occurrence of apical dominance. Studies with a number of taxonomically diverse species have indicated that medium with a cytokinin such as BA is not able to maintain the multiplication rate over multiple subcultures (Berardi et al 1993;Singh and Syamal 2000) and that the inhibition of auxin activity can have a beneficial effect on long-term shoot proliferation.…”

Section: Discussionmentioning

confidence: 99%

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

ShuklaMukund

Alan

et al. 2012

Can. J. For. Res.

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An efficient procedure for the conservation of mature American elm ( Ulmus americana L.) trees that have survived the epidemics of Dutch elm disease and are potential sources of disease resistance is reported. The model utilizes in vitro proliferation of fresh and dormant buds from mature trees for cloning nearly 100 year old American elm trees. The key factors that influenced sustained growth and multiplication included optimization of culture process and auxin metabolism in the source tissue. Blocking the action of endogenous auxins through the addition of antiauxin in the proliferation medium was crucial for high multiplication rate and optimum shoot development. Addition of antiauxin also mitigated the decline in productivity observed with multiple subcultures, which will enable long-term conservation of selected germplasm. The most effective medium for long-term proliferation contained 5.0 µmol/L p-chlorophenoxyisobutyric acid with 2.2 µmol/L benzylaminopurine and 0.29 µmol/L gibberellic acid. Medium with 2.5 µmol/L indole-3-butyric acid was the best for rooting microshoots (89%). Rooted plantlets were readily acclimatized to the greenhouse environment with a 90% survival rate. The strategy developed for American elm will aid in increasing multiplication of resistant clones, facilitate long-term conservation of elite genotypes, and also provide an approach to improve conservation of other endangered tree species.

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Section: Discussionmentioning

confidence: 99%

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

ShuklaMukund

Alan

et al. 2012

Can. J. For. Res.

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“…Moreover, to overcome the apical dominance of in vitro shoots that was strongly linked to the induction of in vitro flowering in 'Finola', 2,3,5-triiodobenzoic acid (TIBA) was tested in combination with TDZ and GA 3 . TIBA is a synthetic inhibitor of auxin flux and has been widely applied in studies on in vitro shoot regeneration, as reported in Rosa hybrida [45], Cucumis sativus [46], and 'Finola' in combination with TDZ [22]. The results of the present study showed an increase of about 1 cm in the length of the shoots compared with the combination of BAP + GA 3 or MT + GA 3 , albeit with a lower multiplication rate than the best mean value achieved with the combination TDZ + NAA (Figure 6a).…”

Section: Discussionmentioning

confidence: 99%

Establishment of an Efficient In Vitro Propagation Protocol for Cannabis sativa L. subsp. ruderalis Janish

Basile,

Tedone,

Pulvento

et al. 2023

Horticulturae

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Cannabis sativa L., subsp. ruderalis Janish., ‘Finola’ is a dioecious cultivar of Finnish origin. This cultivar is very interesting because its cultivation cycle lasts less than 3 months. The aim of this study was to define an efficient micropropagation protocol to ensure in vitro multiplication and rooting and in vivo acclimatization. Two different explant sources were tested: seed-derived in vitro explants and nodal segments containing axillary buds from selected mother plants. Shoot proliferation was tested on different growth media enriched with cytokinin alone or cytokinin in combination with auxins. Among all combinations, the best results were obtained by combining the Basal Medium (BM—a Murashige and Skoog modified medium) with sucrose (20 g L−1), thidiazuron (TDZ 0.4 mg L−1), and 1-naphthalenacetic acid (NAA 0.2 mg L−1). Regarding rooting induction, the plants developed an extensive root system under red/blue lights on BM enriched with sucrose (30 g L−1) and indol-3 butyric acid (0.1 mg L−1), which allowed the survival of more than 90 percent of the plantlets once transplanted into the climate-controlled greenhouse.

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“…Several approaches have been used to reduce callus formation, including cytokinin elimination or the employment of auxin transport inhibitors, such as TIBA. This compound enhanced somatic embryogenesis in groundnut and shoot formation in Morus alba (Venkatesh et al 2009; Bhau and Wakhlu 2001) and enhanced Rosa hybrida micropropagation (Singh and Syamal 2000). Thus, we cultivated the T. mombergeri shoots in ½ WPM-2Ca-P added with 0.5, 1 and 2 mg L − 1 TIBA.…”

Section: Hyperhydricity Can Be Reduced By Improving Ventilation and To Decrease Ethylene Accumulation In Vessels;mentioning

confidence: 99%

In Vitro Regeneration of The Endangered Cactus Turbincarpus Mombergeri Riha, A Hybrid of T. Laui x T. Pseudopectinatus

Santos-Díaz

Alvarado-Rodríguez

2021

Preprint

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Turbinicarpus mombergeri is a cacti species formed by a hybridization process between Turbinicarpus laui and Turbinicarpus pseudopectinatus. Under natural conditions, it is very difficult for two species be genetically compatible for hybridization, and to produce flowers at the same time. Thus, T. mombergeri is a very interesting and a rare species. Unfortunately, the current populations are decreasing and now it is considered critically endangered. The aim of this research was to develop a successful protocol for propagating T. mombergeri using the in vitro culture techniques. Seed disinfection was performed with Plant Preservative Mixture, and 80% of germination occurred at day 45 in Murashige-Skoog medium. The shoots were cut longitudinally, and the segments were transferred to media containing 2.22 or 4.44 µM benzyladenine to induce shooting. The generated shoots were highly hydrated, and presented abundant callus. The hyperhydricity was controlled by reducing salt medium concentration, by increasing calcium levels and by using polyethylenglycol. The reduction of callus was attained by adding tri-iodo benzoic acid. Vigorous and thick shoots were generated in medium containing urea, and rooting improved in the presence of 0.5 µM indoleacetic acid. Plantlets with normal morphology were obtained, and the survival rate of the plants in soil was 80%. The methodology developed represents an alternative for propagation of T. mombergeri under controlled conditions for commercial or conservation purposes.

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Anti-Auxin Enhance Rosa Hybrida L. Micropropagation

Cited by 16 publications

References 15 publications

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

In vitro conservation of American elm (Ulmus americana): potential role of auxin metabolism in sustained plant proliferation

Establishment of an Efficient In Vitro Propagation Protocol for Cannabis sativa L. subsp. ruderalis Janish

In Vitro Regeneration of The Endangered Cactus Turbincarpus Mombergeri Riha, A Hybrid of T. Laui x T. Pseudopectinatus

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