Anti-Bacterial Mechanism for Metallic Ag+, Cu2+, Zn2+ Ions-Induced Bactertiolysis on Disruptive OM Lpp and PGN Inhibitive Elongations Against S. aureus and E. coli

Abstract

Anti-bacterial mechanism for complete-ionized Ag + , Cu 2+ , Zn 2+ ion solutions has been established against S. aureus and E. coli. Anti-bacterial mechanism against S. aureus is involved that bacterolysis and destruction of S. aureus cell wall occur by inhibition of PGN elongation through metallic Ag + , Cu 2+ , Zn 2+ ions-induced PGN inhibitive transglycosylase (TG) and transpeptidase (TP) syntheses (TG for Zn 2+ ) and PGN activated major autolysin of amidase. The other, anti-bacterial mechanism against E. coli has been clarified that bacteriolysis and destruction of E. coli cell wall occur by disruption of E. coli outer membrane (OM) structure with OM lipoprotein-endopeptidase activation, and by inhibition of PGN elongation through inhibitive TG and TP syntheses (TG for Zn 2+ ) and PGN activated major autolysins. Ag + , Cu 2+ , Zn 2+ ions-induced ROS generation of O 2 and H 2 O 2 and ROS-mediated oxidative stress in bacterial cell lead to killing by stress damage for silver ions, cell membrane damages due to high reactive •OH and OHare formed by Haber-Weiss and Fenton reactions for Cu 2+ ions, and DNA molecular damage for Zn 2+ ions.