Anti-biofilm activity of bacteriophages and lysins in chronic rhinosinusitis

Łusiak-Szelachowska, Marzanna; Weber‐Dąbrowska, Beata; Żaczek, Maciej; Górski, Andrzej

doi:10.4149/av_2021_203

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…The inhibition of bacteria in PAO1 and PA143 biofilms by HZ2201 at 24 h reached 72.62 ± 11.11% and 95.61 ± 2.21%, respectively (P<0.05). Phages can synthesize specific enzymes, such as endolysin and depolymerase, to degrade the extracellular polymeric substances (EPSs) of the biofilm ( Olsen et al., 2018 ; Al-Wrafy et al., 2019 ; Lusiak-Szelachowska et al., 2021 ). The dense and persistent biofilm of bacteria is an important reason for the current drug resistance of PA, and bacteria within biofilms are known to be approximately 1000 times more resistant to antibacterial drugs than their free bacterial counterparts ( Srinivasan et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Fei,

Li,

Liu

et al. 2023

Virus Research

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Section: Discussionmentioning

confidence: 99%

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Fei,

Li,

Liu

et al. 2023

Virus Research

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“…In daily clinical practice, identifying susceptible or resistant bacteria relies mainly on classical culturable-dependent methods, which evaluate only planktonic bacteria and selected species according to the culture growth medium. As biofilms usually present high resistance to antibiotics relatively to their planktonic counterparts ( Ceri et al., 1999 ; Ciofu et al., 2017 ; Łusiak-Szelachowska et al., 2021 ), we aimed to explore the antimicrobial resistance relationship of planktonic and biofilms in patients with AECRS.…”

Section: Discussionmentioning

confidence: 99%

Biofilm and Planktonic Antibiotic Resistance in Patients With Acute Exacerbation of Chronic Rhinosinusitis

Sabino

Valera

Santos

et al. 2022

Front. Cell. Infect. Microbiol.

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IntroductionThe recalcitrant nature of patients with acute exacerbation of chronic rhinosinusitis (AECRS) potentially involves persisting colonization of the sinonasal mucosa by bacterial biofilms. Biofilms are known to be highly resistant to antibiotics, which may trigger or maintain chronic inflammation in the sinonasal mucosa. However, little is known about the relationship between the minimum inhibitory concentration (MIC) and antibiofilm concentrations of bacteria obtained from AECRS patients.Material and MethodsThirty bacterial strains from 25 patients with AECRS were identified and underwent MIC determination (VITEK® 2). The planktonic isolates were submitted to an in vitro formation of biofilms (Modified Calgary Biofilm Device) and determination of minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) for amoxicillin, amoxicillin/clavulanic acid, clarithromycin, and levofloxacin. MIC of the planktonic forms was compared with MBIC and MBEC levels, according to the breakpoints established by the Clinical Laboratory Standards Institute guidelines.ResultsThe main bacteria retrieved was S. aureus (60%), followed by other Gram-positive and Gram-negative bacteria in lower frequencies. 76.7% of strains formed biofilm in vitro (n=23/30). The planktonic isolates presented high rates of resistance for amoxicillin (82.6%) and clarithromycin (39.1%), and lower rates for amoxicillin/clavulanic acid (17.4%). The biofilm-forming bacteria counterparts presented higher levels of MBIC and MBEC compared to the MIC levels for amoxicillin, amoxicillin/clavulanic acid, and clarithromycin. Levofloxacin was highly effective against both planktonic and biofilm forms. Planktonic resistant forms were associated with levels of antibiofilm concentrations (MBIC and MBEC).ConclusionsBiofilm-forming bacteria from AECRS patients are prevalent, and biofilm forms are highly resistant to antibiotics compared to their planktonic counterparts. Antibiotic resistance observed in planktonic forms is a good indicator of biofilm resistance, although near 20% of susceptible planktonic bacteria can produce antibiotic tolerant biofilms.

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“…Apart from intact phages, phage-derived proteins, such as lysins, have been shown to be efficient in the removal of preformed biofilms. For instance, Staphylococcus lysin P128 was found to be active against Methicillin-sensitive Staphylococcus aureus (MSSA) and Methicillin-resistant Staphylococcus aureus (MRSA) strains [29]. In a similar study, an engineered phage-based lysin, LysAB2, was shown to be active against Acinetobacter baumannii and could disrupt its biofilm formed on a 96-well plate at the end of 24 h [30].…”

Section: Introductionmentioning

confidence: 94%

An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis

et al. 2023

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Multidrug-resistant bacterial infections are on the rise around the world. Chronic infections caused by these pathogens through biofilm mediation often complicate the situation. In natural settings, biofilms are often formed with different species of bacteria existing synergistically or antagonistically. Biofilms on diabetic foot ulcers are formed predominantly by two opportunistic pathogens, Staphylococcus aureus and Enterococcus faecalis. Bacteriophages and phage-based proteins, including endolysins, have been found to be active against biofilms. In this study, we evaluated the activity of two engineered enzybiotics either by themselves or as a combination against a dual biofilm formed by S. aureus and E. faecalis in an inert glass surface. An additive effect in rapidly disrupting the preformed dual biofilm was observed with the cocktail of proteins, in comparison with mono treatment. The cocktail-treated biofilms were dispersed by more than 90% within 3 h of treatment. Apart from biofilm disruption, bacterial cells embedded in the biofilm matrix were also effectively reduced by more than 90% within 3 h of treatment. This is the first instance where a cocktail of engineered enzybiotics has been effectively used to impede the structural integrity of a dual biofilm.

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Anti-biofilm activity of bacteriophages and lysins in chronic rhinosinusitis

Cited by 8 publications

References 42 publications

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Biofilm and Planktonic Antibiotic Resistance in Patients With Acute Exacerbation of Chronic Rhinosinusitis

An Enzybiotic Cocktail Effectively Disrupts Preformed Dual Biofilm of Staphylococcus aureus and Enterococcus faecalis

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