Anti-biofilm agents from marine biota

G. Vladkova, Todorka; L. Martinov, Boris; N. Gospodinova, Dilyana

doi:10.59957/jctm.v58i5.117

Cited by 5 publications

(1 citation statement)

References 76 publications

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“…Representatives of the first group include defensins, cathelicidins, and lactoferricin B. A significant part of the amphibian defense against pathogens is provided by own-produced AMPs [ 9 ]. The well-known first group magainins are isolated from frogs [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Studies of New Temporin A Analogs Containing Unnatural Amino Acids in Position 7

Dimitrova,

Nemska,

Foteva

et al. 2024

Pharmaceutics

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(1) Background: Antimicrobial resistance is growing at an extreme pace and has proven to be an urgent topic, for research into alternative treatments. Such a prospective possibility is hidden in antimicrobial peptides because of their low to no toxicity, effectiveness at low concentrations, and most importantly their ability to be used for multiple treatments. This work was focused on the study of the effect of the modification in position 7 of Temporin A on its biological activity; (2) Methods: The targeted peptides were synthesized using Fmoc/Ot-Bu SPPS. The antibacterial activity of the analogs was determined using the broth microdilution method and disk-diffusion method. In vitro tests were performed to determine the cytotoxicity, phototoxicity, and antiproliferative activity of the peptide analogs on a panel of tumor and normal cell lines; (3) Results: All analogs except DTCit showed good antibacterial activity, with DTDab having the best activity according to the disk-diffusion method. However, DTCit had an acceptable cytotoxicity, combined with good selectivity against the test MCF-7 cell line; (4) Conclusions: The obtained results revealed the importance of the basicity and length of the side chain at position 7 in the Temporin A sequence for both tested activities.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Studies of New Temporin A Analogs Containing Unnatural Amino Acids in Position 7

Dimitrova,

Nemska,

Foteva

et al. 2024

Pharmaceutics

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Recent Progress in Terrestrial Biota-Derived Anti-Biofilm Agents for Medical Applications

Vladkova,

Smani,

Martinov

et al. 2024

Applied Microbiology

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The terrestrial biota is a rich source of biologically active substances whose anti-biofilm potential is not studied enough. The aim of this review is to outline a variety of terrestrial sources of antimicrobial agents with the ability to inhibit different stages of biofilm development, expecting to give some ideas for their utilization in improved anti-biofilm treatments. It provides an update for the last 5 years on anti-biofilm plant products and derivatives, essential oils, antimicrobial peptides, biosurfactants, etc., that are promising candidates for providing novel alternative approaches to combating multidrug-resistant biofilm-associated infections. Based on the reduction in bacterial adhesion to material and cell surfaces, the anti-adhesion strategy appears interesting for the prevention of bacterial attachment in combating a broad range of mono- and multispecies bacterial biofilms. So far, few studies have been carried out in this direction. Anti-biofilm coatings made by or containing biologically active products from terrestrial biota have scarcely been studied although they are of significant interest for a reduction in infections associated with medical devices. Combination therapy with commercial antibiotics and natural products is accepted now as a promising base for future advances in anti-biofilm treatment. In vivo testing and clinical trials are necessary for clinical application.

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A Potent Antibacterial Peptide (P6) from the De Novo Transcriptome of the Microalga Aureococcus anophagefferens

Zhang,

Yin,

Huang

et al. 2024

IJMS

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Marine microalgae are a rich source of natural products, and their amino acid-based antimicrobial agents are usually obtained by enzymatic hydrolysis, which is inefficient and limits the research on antimicrobial peptides (AMPs) from microalgae. In this study, Aureococcus anophagefferens is used as a model to predict antimicrobial peptides through high-throughput methods, and 471 putative peptides are identified based on the de novo transcriptome technique. Among them, three short peptides, P1, P6, and P7 were found to have antimicrobial activity against Escherichia coli, Staphylococcus aureus, Micro1coccus luteus, and yeast Pichia pastoris, and they showed no hemolytic activity even at higher concentrations up to 10 mg/mL. Especially P6, a 12-amino acid peptide with three positive charges, which exhibited the most significant microbicidal effect with the lowest MIC of 31.25 μg/mL against E. coli, and electron microscope observations showed the surface of P6 treated E. coli with granular protrusions and ruptures, suggesting that it likely caused cell death by directly destroying the bacterial cell membrane. This study may enrich the database of microalgal AMPs and demonstrate an efficient process for searching and validating microalgal source AMPs by combining computer analysis with bioactivity experiments.

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Anti-biofilm agents from marine biota

Cited by 5 publications

References 76 publications

Synthesis and Biological Studies of New Temporin A Analogs Containing Unnatural Amino Acids in Position 7

Synthesis and Biological Studies of New Temporin A Analogs Containing Unnatural Amino Acids in Position 7

Recent Progress in Terrestrial Biota-Derived Anti-Biofilm Agents for Medical Applications

A Potent Antibacterial Peptide (P6) from the De Novo Transcriptome of the Microalga Aureococcus anophagefferens

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