Anti-biofilm potential of Lactobacillus plantarum Y3 culture and its cell-free supernatant against multidrug-resistant uropathogen Escherichia coli U12

Mekky, Asmaa F.; Hassanein, Wesam A.; Reda, Fayiz M.; El-Sayed, Hanan M.

doi:10.1016/j.sjbs.2022.01.014

Cited by 17 publications

(10 citation statements)

References 42 publications

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“…paracasei SP5. Similarly, antibiofilm activity of CFCS derived from probiotic LAB was also reported in previous studies against S. aureus ( Lee et al, 2020), uropathogenic E. coli U12 (Mekky et al, 2022) and S. enterica subsp. enterica serovar Enteritidis (Tazehabadi et al, 2021).…”

Section: Discusssionsupporting

confidence: 81%

Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria

Kiousi

Efstathiou²,

Tzampazlis³

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionLactobacilli are avid producers of antimicrobial compounds responsible for their adaptation and survival in microbe-rich matrices. The bactericidal or bacteriostatic ability of lactic acid bacteria (LAB) can be exploited for the identification of novel antimicrobial compounds to be incorporated in functional foodstuffs or pharmaceutical supplements. In this study, the antimicrobial and antibiofilm properties of Lactiplantibacillus pentosus L33, Lactiplantibacillus plantarum L125 and Lacticaseibacillus paracasei SP5, previously isolated form fermented products, were examined, against clinical isolates of Staphylococcus aureus, Salmonella enterica subsp. enterica serovar Enteritidis and Escherichia coli.MethodsThe ability of viable cells to inhibit pathogen colonization on HT-29 cell monolayers, as well as their co-aggregation capacity, were examined utilizing the competitive exclusion assay. The antimicrobial activity of cell-free culture supernatants (CFCS) was determined against planktonic cells and biofilms, using microbiological assays, confocal microscopy, and gene expression analysis of biofilm formation-related genes. Furthermore, in vitro analysis was supplemented with in silico prediction of bacteriocin clusters and of other loci involved in antimicrobial activity.ResultsThe three lactobacilli were able to limit the viability of planktonic cells of S. aureus and E. coli in suspension. Greater inhibition of biofilm formation was recorded after co-incubation of S. enterica with the CFCS of Lc. paracasei SP5. Predictions based on sequence revealed the ability of strains to produce single or two-peptide Class II bacteriocins, presenting sequence and structural conservation with functional bacteriocins.DiscussionThe efficiency of the potentially probiotic bacteria to elicit antimicrobial effects presented a strain- and pathogen-specific pattern. Future studies, utilizing multi-omic approaches, will focus on the structural and functional characterization of molecules involved in the recorded phenotypes.

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Section: Discusssionsupporting

confidence: 81%

Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria

Kiousi

Efstathiou²,

Tzampazlis³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Additionally, ( El-Mokhtar et al, 2020 ) reported that the CFS of lactobacilli demonstrated a high anti-ESBL with inhibition zone diameters greater than 13 mm in the agar well assays against both K. pneumoniae and P. aeruginosa . Similarly, it was indicated that the CFS of L. plantarum Y3 showed a great inhibitory effect against MRD uropathogenic E. coli U12 ( Mekky et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60

El-Mekkawy

Hamour

Hassanein

et al. 2023

Saudi Journal of Biological Sciences

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“…Probiotics not only hinder the activity of pathogenic bacteria and their adhesion to surfaces, they also prevent the formation and survival of pathogenic bio lms, interfere with bio lm integrity and eventually lead to bio lm eradication [44]. It has been shown that L. plantarum has the ability to inhibit the formation of pathogenic bacterial bio lms on catheters when applied to catheters [45]. This was also con rmed in the present study, where we found that honey and its combination with L. plantarum had a good inhibitory effect on bio lm formation of S. aureus and P. aeruginosa.…”

Section: Discussionmentioning

confidence: 99%

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Xiao

Su³

et al. 2022

Preprint

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The prevention and control of infection by resistant pathogens has become a formidable challenge due to the increasing resistance of pathogens to antibiotics, indicating the urgent need for new effective antimicrobial agents. Probiotic is a potential antibacterial drug that can address pathogenic infections. In this study, the antibacterial formulation consisting of honey and Lactobacillus plantarum (L. plantarum) was investigated the optimal antibacterial formulation, in vitro antibacterial effect and mechanism, as well as their effect on the healing of infected wounds in rat whole skin. The results of in vitro experiments showed that the optimal antibacterial formulation of honey and L. plantarum was: honey 10% + L. plantarum 1×109 CFU/mL. In vitro counts showed that the formulation inhibited more than 80% of Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli) after 24 h of action. In addition, live bacterial counts and growth curves indicate that L. plantarum used honey to promote its own growth and produce acids to inhibit the survival of pathogenic bacteria and biofilm formation(P < 0.001). Furthermore, in vivo results reviewed that the honey- L. plantarum formulation inhibited wound bacterial growth(P < 0.01) and increased wound epithelial granulation tissue formation to accelerate skin wound healing in a rat bacterial infection wound model. Overall, these findings suggest that the honey- L. plantarum formulation provides a new option for the treatment of pathogenic infections and wound healing, which sheds new light on the search for alternatives to antibiotics in the future.

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Anti-biofilm potential of Lactobacillus plantarum Y3 culture and its cell-free supernatant against multidrug-resistant uropathogen Escherichia coli U12

Cited by 17 publications

References 42 publications

Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria

Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria

Evaluation of the antibacterial activity of Weissella confusa K3 cell-free supernatant against extended-spectrum βeta lactamase (ESBL) producing uropathogenic Escherichia coli U60

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

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