Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation

Sari, Roger Shoji; Almeida, Anna Christina de; Cangussu, Alex Sander Rodrigues; Jorge, Edson V.; Mozzer, O. D.; Santos, Hércules Otacílio; Quintilio, Wagner; Brandi, Igor Viana; Andrade, Viviane A.; Miguel, Ana Carolina Almeida; Santos, Eliane M. Sobrinho

doi:10.1016/j.anaerobe.2016.10.013

Cited by 14 publications

(9 citation statements)

References 20 publications

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“…The present study represents an innovative and simple technological strategy for the emulsification of inactivated toxins as immunogen 9,10 . Polymers have been used in bovine vaccine such as herpesvirus 1 (BHV-1), infectious bovine rhinotracheitis (IBR), and inactivated C. botulinum toxin with protection and safety being similar to that of the conventional vaccine 15–17 . As a proof of concept in this murine model, we consider it to be a relevant technological contribution of the use of nano-iCnB for immunization of Swiss mice.…”

Section: Discussionmentioning

confidence: 99%

Inactivated alpha toxin from Clostridium novyi type B in nano-emulsion protect partially protects Swiss mice from lethal alpha toxin challenge

Felix

Deusdará

Santos

et al. 2019

Sci Rep

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Nano-emulsions are promising carriers for antigen delivery. Here, we evaluated the efficacy of a water-oil nano-emulsion containing concentrated, inactivated Clostridium novyi (C. novyi) type B supernatant culture (nano-iCnB) in protecting Swiss mice against a lethal dose of alpha toxin concentrated extract. Proteins were confirmed in the nano-iCnB and their stabilities were determined according physical parameters such as Zeta Potential (ZP). Biochemical, hematological parameters and morphological appearance of liver, spleen and thigh muscle alterations were examined to determine the safety of the compound. Partial protection against lethal doses was achieved in immunized mice despite low IgG titers. These data suggest that our nano-emulsion is a simple and efficient method of promoting antigen delivery for toxin-related diseases.

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Section: Discussionmentioning

confidence: 99%

Inactivated alpha toxin from Clostridium novyi type B in nano-emulsion protect partially protects Swiss mice from lethal alpha toxin challenge

Felix

Deusdará

Santos

et al. 2019

Sci Rep

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“…Complex coacervation, [49,51,52,56,57,[61][62][63][64][65][66][67][68][69][70][71][72][73] whose characteristics mimic the characteristics of cells, is a simple, purely aqueous strategy for encapsulating proteins. Complex coacervation is a liquid-liquid phase separation phenomenon resulting from the association of two oppositely charged macro-ions (e.g., polymer, protein, surfactant micelles).…”

Section: Complex Coacervation Phase Behavior and Biomimicrymentioning

confidence: 99%

“…However, to the best of our knowledge, only the Bradford and BCA assays have been used in the context of quantifying proteins in complex coacervates. [51,52,72,91] Although the coacervating polypeptides poly(lysine) and poly(glutamate) have been shown to work for the Bradford assay, [51,52] these same materials are not compatible with the BCA reagents, and more typical synthetic polymers such as poly(styrene sulfonate), poly(diallyldimethylammonium chloride), and other methacrylate based polymers have strong interactions with the assay reagents that overwhelm the ability of an experiment to detect protein. For a more detailed overview of these assays, we refer the reader to Ref.…”

Section: Measuring Protein Concentrationmentioning

confidence: 99%

Protein Encapsulation Using Complex Coacervates: What Nature Has to Teach Us

McTigue

Perry

2020

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112

128

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Protein encapsulation is a growing area of interest, particularly in the fields of food science and medicine. The sequestration of protein cargoes has been achieved using a variety of methods, each with benefits and drawbacks. One of the most significant challenges associated with protein encapsulation has been achieving high loading while maintaining protein viability. This difficulty has been exacerbated because many encapsulant systems require the use of organic solvents. In contrast, nature has optimized strategies to compartmentalize and protect proteins inside the cell-a purely aqueous environment. Although the mechanisms whereby aspects of the cytosol is able to stabilize proteins are unknown, the crowded nature of many newly discovered, liquid phase separated 'membraneless organelles' that achieve protein compartmentalization suggests that the material environment surrounding the protein may be critical in determining stability. Here, we focus on encapsulation strategies based on liquid-liquid phase separation, and complex coacervation in particular, which has many of the key features of the cytoplasm as a material. We review the literature on protein encapsulation via coacervation and discuss the parameters relevant to creating protein-containing coacervate formulations. Additionally, we highlight potential opportunities associated with the creation of tailored materials to better facilitate protein encapsulation and stabilization.

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“…In a study undertaken by Sari and colleagues [47], chitosan was used as adjuvant and encapsulating material for the formulation of an anti-botulism single shot vaccine. The toxoids type C and D were encapsulated in chitosan by coacervation method using sodium sulfate as the precipitating agent.…”

Section: Encapsulation Of Biologicsmentioning

confidence: 99%

Natural Polymers in Micro- and Nanoencapsulation for Therapeutic and Diagnostic Applications: Part II - Polysaccharides and Proteins

Ngwuluka¹,

Abu-Thabit²,

Uwaezuoke³

et al. 2021

Nano- And Microencapsulation - Techniques and Applications

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Encapsulation remains a fundamental and consistent approach of fabrication of drug and diagnostic delivery systems in the health space and natural polymers such as polysaccharides and proteins continue to play significant roles. Micro- or nanoencapsulation is employed for the conventional pharmaceuticals, biopharmaceuticals, or biologics, bioactives from natural sources and diagnostics such as biomarkers. The outcome of any encapsulation depends on the technique employed and the encapsulating material. The encapsulating materials employed influence the physical and chemical attributes of the fabricated micro- and nanocapsules. The encapsulating materials could be natural or synthetic, however, natural polymers are preferred because they are human and environmentally friendly. Polysaccharides and proteins are abundant in nature, biogenic, biocompatible, biodegradable and possess biological functions making them materials of choice for encapsulation of drugs and diagnostics. This chapter reviews the recent and advanced applications of polysaccharides and proteins as nanocarrier materials for micro- and nanoencapsulation of therapeutics and diagnostics.

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Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation

Cited by 14 publications

References 20 publications

Inactivated alpha toxin from Clostridium novyi type B in nano-emulsion protect partially protects Swiss mice from lethal alpha toxin challenge

Inactivated alpha toxin from Clostridium novyi type B in nano-emulsion protect partially protects Swiss mice from lethal alpha toxin challenge

Protein Encapsulation Using Complex Coacervates: What Nature Has to Teach Us

Natural Polymers in Micro- and Nanoencapsulation for Therapeutic and Diagnostic Applications: Part II - Polysaccharides and Proteins

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