Anti-cancer & anti-metastasis properties of bioorganic-capped silver nanoparticles fabricated from Juniperus chinensis extract against lung cancer cells

Abstract

The current study evaluated the anti-cancer properties of bio-functionalized silver nanoparticles fabricated by Juniperus chinensis leaf extracts. The nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, dynamic light scattering, Zeta potential and X-ray spectroscopy. Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. The size of the spherical nanoparticle was 12.96 nm with negative zeta potential. Up-regulation of caspase 3,9 and p53, Annexin V-FITC/PI, DAPI staining, and ROS production indicated the remarkable apoptotic effect of AgNPs compared to cisplatin. Moreover, down-regulation of MMP2/MMP9 scratch and matrigel assays revealed anti-metastatic properties of AgNPs. Cell cycle analysis and downregulation of cyclin D1 indicated cancer cell cessation in the G0/G1 phase. Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line.

Novel Antibacterial, Cytotoxic and Catalytic Activities of Silver Nanoparticles Synthesized from Acidophilic Actinobacterial SL19 with Evidence for Protein as Coating Biomolecule

“…Similarly, Noorbazargan et al . [ 78 ] presented the cytotoxicity of AgNPs biosynthesized by Juniperus chinensis leaf extract on MCF-7 tumor cells at a dose of 12.5 μg/ml. The most pronounced effect out of all tested cell lines was the ROS generation by biosynthesized AgNPs observed in HepG2 cells ( Figs.…”

“…They tested AgNPs from aqueous extract of seaweed Gracilaria corticata and found that nanosilver at doses of 6.25 and 12.5 μg/ml effectively induced a toxic effect in HepG2 cells. Similarly, Noorbazargan et al [78] presented the cytotoxicity of AgNPs biosynthesized by Juniperus chinensis leaf extract on MCF-7 tumor cells at a dose of 12.5 μg/ml. The most pronounced effect out of all tested cell lines was the ROS generation by biosynthesized AgNPs observed in HepG2 cells (Figs.…”

Preparation of triangular silver nanoparticles and their biological effects in the treatment of ovarian cancer

“…Relevant studies have shown that AgNPs activate caspase-3 and promote cell apoptosis, thereby inhibiting tumour growth [ 37 , 38 ] . Studies have confirmed that AgNPs increase the caspase-3 level in breast cancer [ 39 ], lung cancer [ 40 ] and other cancers, indicating that tAgNP-treated OC cells complete the apoptosis process through the cysteine protease pathway.…”

Targeting the ACE2 receptor using nanomedicine: Novel approach to lung cancer therapy

“…Silver NPs (AgNPs) have been shown to have better physical, chemical, and biological properties than their bulk form. AgNPs offer ease of synthesis, good chemical stability, biocompatibility, optical, thermal, and electrical conductivity, as well as antiviral, anti-inflammatory, antifungal, antibac-terial, anticancer, antiangiogenic and catalytic properties [28][29][30] . NSCLC cells treated with AgNPs showed significant dose-dependent growth inhibition in vitro, with stimulation of apoptosis indicating a potential for chemotherapy and chemoprevention in vivo [31] .…”