Anti-cancer effects of ethanol extract of Reynoutria japonica Houtt. radix in human hepatocellular carcinoma cells via inhibition of MAPK and PI3K/Akt signaling pathways

Kim, Ba Reum; Ha, Jain; Lee, Sewoong; Park, Jiyoung; Cho, Sayeon

doi:10.1016/j.jep.2019.112179

Cited by 13 publications

(6 citation statements)

References 93 publications

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“…In this study, a total of 12 herbal medicines such as Rhizoma Coptidis, Folium Isatidis, Acidum Citricum, Radix Stellariae, T. fragiferum and 27 herbal ingredients such as resveratrol, cinnamaldehyde, oleuropein, shikonin and magnolol, were directly predicted based on the key genes TLR4 and KRAS. In fact, some of the predicted herbs such as Rhizoma Coptidis (Chai et al, 2018;Kim et al, 2019), Rhizoma Polygoni cuspidati (Jiang et al, 2017) and Radix Salviae liguliobae (Wilke et al, 2014) have been shown to have significant anti-gastric cancer effects. Predicted anti-gastric cancer effects of herbal ingredients such as taxol (Huang et al, 2020b;Zhang et al, 2021), coumarin (Kaur et al, 2015;Xu et al, 2017), resveratrol (Yan et al, 2020;Ren et al, 2021) and magnolol (Zhao et al, 2022) have also been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Development of alternative herbals remedy for gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis

Tao²,

Qian³

et al. 2023

Front. Genet.

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Objective: Screening out potential herbal medicines and herbal ingredients for the treatment of gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis.Methods: Gene expression profiles of gastric tumour tissues and normal tissue samples were obtained from the GEO database and the samples were analysed for immune cell infiltration condition and differential expressed genes of ferroptosis. Key genes were screened by protein-protein interaction (PPI) and enrichment analysis, and molecular docking was used to predict and preliminary validate potential herbal and traditional Chinese medicine components for gastric cancer based on the key genes. Finally, RT-QPCR was used to validate the prediction results.Results: Immune cell infiltration analysis revealed high levels of infiltration of activated CD4 memory T cells, monocytes, M0 macrophages in gastric tumor tissues, while plasma cells and resting mast cells had higher levels of infiltration in the paraneoplastic tissues. Differential gene expression analysis identified 1,012 upregulated genes and 880 downregulated genes, of which 84 immune related differentially expressed genes such as CTSB, PGF and PLAU and 10 ferroptosis-related differentially expressed genes such as HSF1, NOX4 and NF2 were highly expressed in gastric cancer tissues. The results of enrichment analysis showed that they mainly involve 343 biological processes such as extracellular matrix organization and extracellular structural organization; 37 cellular components such as complexes of collagen trimer and basement membrane; 35 molecular functions such as signal receptor activator activity and receptor ligand activity; 19 regulatory pathways such as cytokine-cytokine receptor interactions and retinol metabolism. Finally, two key genes, TLR4 and KRAS, were selected and 12 herbal medicines such as Radix Salviae liguliobae, Rhizoma Coptidis, Rhizoma Polygoni cuspidati and 27 herbal ingredients such as resveratrol, salvianolic acid b were predicted on the basis of key genes. Molecular docking results showed that KRAS binds tightly to coumarin and magnolol, while TLR4 can bind tightly to resveratrol, curcumin, salvianolic acid b, shikonin. Subsequently, the effect of resveratrol and magnolol was experimentally verified.Conclusion: Herbal medicines such as S. liguliobae, Rhizoma Coptidis, Rhizoma P. cuspidati and herbal ingredients such as resveratrol, curcumin, salvianolic acid b may provide research directions and alternative therapeutic approaches for immunomodulation of TME and ferroptosis of tumour cells in gastric cancer.

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Section: Discussionmentioning

confidence: 99%

Development of alternative herbals remedy for gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis

Tao²,

Qian³

et al. 2023

Front. Genet.

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“…91 . Shoots (2 cm height) from clusters previously micropropagated in vitro were used as explants and subcultured in vitro to elongation and rooting for 90 days, free of plant growth regulators, up to the formation of a complete explant (basal and aerial part)-becoming available for extraction of leaves compounds, protocol adapted from Kim et al 80 . Plantlets were removed from culture chambers and the leaves were cut into small pieces and 1 g of fresh leaf mass was macerated and extracted in 10 mL of specific solvent, over 24 hours, under 80 rpm agitation and 25 °C in a dark room.…”

Section: Discussionmentioning

confidence: 99%

“…The antitumoral activity was attibuted to cysteine proteinases (e.g., bromelain and fastuosain) as well as flavonoids, including penduletin, cirsimaritin and HLBT-100 33,79 . Biological compounds are related to the suppression of some metastatic markers, resulting in regulation of mitogen activated protein kinase and protein kinase B 80 . Genistein, present in higher amounts in both HA and CA (Table 4), is also related to the inhibition of protein tyrosine kinase and topoisomerase II, and elimination of oxygen free radicals 81,82 , inhibiting the bioavailability of sex hormones, platelet aggregation, angiogenesis, as well as modulating the apoptosis of malignant cell lines 83 .…”

Section: Chlorophyll Quantification Leaves Ofmentioning

confidence: 99%

Phytochemical analysis and biological activities of in vitro cultured Nidularium procerum, a bromeliad vulnerable to extinction

Gollo

Tanobe

Pereira

et al. 2020

Sci Rep

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In this study, Response surface methodology (RSM) was applied to optimize the yield of crude methanolic extract of Mitragyna speciosa leaves using Ultrasound-assisted extraction (UAE). The crude methanolic extract and its fractions were quanti ed in terms of total phenolic content and total avonoid content, along with characterized using Fourier-transform infrared and Gas chromatography-mass spectrometry. The results showed the maximum yield of 49.72% at the optimal conditions (temperature, 34 °C; time, 25 min; and volume of solvent, 166 mL). The recovery crude methanolic extract for TPC and TFC were 137.3 ± 15.7 mg GAE/g and 90.3 ± 15.3 mg RE/g, respectively.

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“…The PI3K/Akt axis is well known to participate in the cell growth and survival of various types of cancers, including RB ( 36 – 38 ). A previous study reported that the ethanol extracts of R. japonica radix (ERJR) could play an inhibitory effect on hepatocellular carcinoma metastasis via PI3K/Akt signaling pathways ( 39 ). PTEN is reported to act as an antagonist of PI3K action, it regulates PI3K/Akt signaling negatively ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

miR‑494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway

Liu

Wang

et al. 2020

Oncol Lett

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Increasing evidence has indicated that the dysregulation of microRNA (miRNA) occur in the pathogenesis of retinoblastoma (RB). Aim of the present study was to investigate the possible role of miR-494 (miR-494-3p) in RB. It was demonstrated that miR-494 expression was increased in RB tissue samples and cell lines. Also, it was prominently associated with clinicopathological features. Functional assays showed that RB cell proliferation, invasion and migration can be promoted by miR-494 overexpression. Besides, phosphatase and tensin homolog (PTEN) was verified as a possible target of miR-494 by a luciferase assay, western blot and qRT-PCR assay in RB. miR-494 and PTEN expression was negatively related in a correlation analysis on tumor tissues of 66 patients. In addition, PTEN was proved to reverse miR-494 effect on RB cell progression. Moreover, PI3K/AKT signaling pathway was validated to take part in RB progression. Taken together, the current study proposes that miR-494 might function as a tumor promoter and regulates RB progression through targeting PTEN.

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Anti-cancer effects of ethanol extract of Reynoutria japonica Houtt. radix in human hepatocellular carcinoma cells via inhibition of MAPK and PI3K/Akt signaling pathways

Cited by 13 publications

References 93 publications

Development of alternative herbals remedy for gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis

Development of alternative herbals remedy for gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis

Phytochemical analysis and biological activities of in vitro cultured Nidularium procerum, a bromeliad vulnerable to extinction

miR‑494 promotes progression of retinoblastoma via PTEN through PI3K/AKT signaling pathway

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