Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells

Zhong, Zhangfeng; Yu, Haibing; Wang, Shengpeng; Wang, Ying; Cui, Liao

doi:10.1186/s13020-018-0203-z

Cited by 25 publications

(16 citation statements)

References 39 publications

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“…Western blotting assays were conducted according to previous studies [15]. In brief, RAW 264.7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL) for 4 h followed by the addition of LPS (1 μg/mL) for 12 h. The cells were harvested, and the total proteins were extracted with RIPA lysis buffer after the desired drug treatment.…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

et al. 2019

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Background Sigesbeckia glabrescens Makino (SG) is one of the important plant origins of Sigesbeckiae herba and has been widely used for the treatment of chronic inflammatory diseases in China. However, the underlying anti-inflammatory mechanism of SG is rarely investigated and reported. There are more than 40 kinds of chemical constituents in SG, but the action of the bioactive compounds of SG is still unclear. Therefore, we aimed to systemically investigate the mechanisms behind the anti-inflammatory properties of SG by combining in vitro and in silico investigations. Methods Cytotoxicity was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Nitric oxide (NO) release was detected using the Griess assay. The secretion of pro-inflammatory cytokines and the expression of relevant proteins were assessed using ELISA kits and Western blots, respectively. Molecular docking was performed and scored using AutoDock via a comparison with the molecular docking of N-acetyl-d-glucosamine (NAG). Results In lipopolysaccharides (LPS)-stimulated macrophages, SG significantly inhibited NO, MCP-1, and IL-6 secretion; iNOS expression; and NF-κB activation but did not significantly affect MAPK signalling (p38, ERK, and JNK). Moreover, the results from the molecular docking prediction suggested that over 10 compounds in SG could likely target TLR4, p105, and p65. Conclusions These findings suggest that the anti-inflammatory effects of SG are highly related to the inactivation of NF-κB. Moreover, this study provides a novel approach to investigate the effects of herbal medicine using combined in vitro and in silico investigations.

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Section: Methodsmentioning

confidence: 99%

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

et al. 2019

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“…Cancer is a leading public health problem worldwide with an estimated 18.1 million new cases and 9.6 million cancer deaths in 2018 [1]. Chinese herbal medicine has been used as anti-cancer agents for a long time, they exhibit anti-inflammatory activities and contain abundant anticancer compounds that exert direct cytotoxicity effects and indirect regulation in tumor microenvironment and cancer immunity, as well as improve chemotherapy [2][3][4][5]. For examples, PNAS reported that epigallocatechin gallate (EGCG) targeting Laminin receptor (Lam 67R) shows promising efficacy in treating prostate cancer [6].…”

Section: Introductionmentioning

confidence: 99%

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

et al. 2019

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Numerous natural products originated from Chinese herbal medicine exhibit anti-cancer activities, including antiproliferative, pro-apoptotic, anti-metastatic, anti-angiogenic effects, as well as regulate autophagy, reverse multidrug resistance, balance immunity, and enhance chemotherapy in vitro and in vivo. To provide new insights into the critical path ahead, we systemically reviewed the most recent advances (reported since 2011) on the key compounds with anti-cancer effects derived from Chinese herbal medicine (curcumin, epigallocatechin gallate, berberine, artemisinin, ginsenoside Rg3, ursolic acid, silibinin, emodin, triptolide, cucurbitacin B, tanshinone I, oridonin, shikonin, gambogic acid, artesunate, wogonin, β-elemene, and cepharanthine) in scientific databases (PubMed, Web of Science, Medline, Scopus, and Clinical Trials). With a broader perspective, we focused on their recently discovered and/or investigated pharmacological effects, novel mechanism of action, relevant clinical studies, and their innovative applications in combined therapy and immunomodulation. In addition, the present review has extended to describe other promising compounds including dihydroartemisinin, ginsenoside Rh2, compound K, cucurbitacins D, E, I, tanshinone IIA and cryptotanshinone in view of their potentials in cancer therapy. Up to now, the evidence about the immunomodulatory effects and clinical trials of natural anti-cancer compounds from Chinese herbal medicine is very limited, and further research is needed to monitor their immunoregulatory effects and explore their mechanisms of action as modulators of immune checkpoints.

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“…Exponentially growing cells were seeded into 96-well plates at a density of 3,000 cells/well in 100-μl medium and allowed to attach overnight. Cells were treated with designated drugs or drug combinations for 24 h. Cell viability was assessed using MTT method as previously described (Zhong et al, 2018). The concentration for half maximum inhibitory effect (IC 50 ) was calculated by GraphPad Prism 5.…”

Section: Methodsmentioning

confidence: 99%

“…However, although patients are responsive to initial drug treatment, progressive disease occurs invariably, and the response rate becomes decreased due to the occurrence of multidrug resistance (MDR). The main mechanisms of MDR include the increased drug efflux, deregulated apoptosis or survival, alteration of drug targets, as well as adapted metabolic reprogramming (Kesharwani et al, 2018; Zhang et al, 2018; Zhong et al, 2018; Zhou et al, 2019). In most cases, multiple MDR impairments may simultaneously occur, which gives rise to challenge in successful therapies in cancer.…”

Section: Introductionmentioning

confidence: 99%

A Danshensu-Tetramethylpyrazine Conjugate DT-010 Overcomes Multidrug Resistance in Human Breast Cancer

Zhou

Wang

et al. 2019

Front. Pharmacol.

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Background: We previously demonstrated that a Danshensu-Tetramethylpyrazine conjugate DT-010 enhanced anticancer effect of doxorubicin (Dox) in Dox-sensitive human breast cancer cells, and protected against Dox-induced cardiotoxicity. This work was designed to see whether DT-010 overcomes Dox resistance in resistant human breast cancer cells. Methods: The effects of DT-010, Dox or their combination on cell viability of Dox-resistant human breast cancer MCF-7/ADR cells were conducted using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was examined by flow cytometry after Annexin V-FITC/PI co-staining. Dox accumulation in MCF-7/ADR cells was detected by flow cytometry and fluorescence microscopy. A fluorometric multidrug resistance (MDR) assay kit was used to evaluate the effect of DT-010 on MDR transporter activity. P-glycoprotein (P-gp) expression and activity were analyzed by Western blot and rhodamine 123 (Rh123) efflux assay, respectively. The effects of DT-010 on glycolysis and mitochondrial stress were detected using an Extracellular Flux Analyzer. A Succinate Dehydrogenase Activity Assay kit was used to measure mitochondrial complex II activity. Results: At non-cytotoxic concentrations, DT-010 in combination with Dox led to a significant growth inhibition of MCF-7/ADR cells, suggesting a synergy between DT-010 and Dox to reverse Dox resistance. DT-010 restored Dox-mediated apoptosis and p53 induction in MCF-7/ADR cells. DT-010 increased Dox accumulation in MCF-7/ADR cells via inhibiting P-gp activity, but without changing P-gp expression. Further studies showed that DT-010 significantly inhibited glycolysis and mitochondrial function of MCF-7/ADR cells. Mitochondrial complex II activity was inhibited by DT-010 or DT-010/Dox combination, but not by Dox. The DT-010-mediated suppression of metabolic process may render cells more vulnerable to Dox treatment and thus result in enhanced efficacy. Conclusions: The results indicate that DT-010 overcomes Dox resistance in human breast cancer cells through a dual action via simultaneously inhibiting P-gp-mediated drug efflux and influencing metabolic process.

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Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells

Cited by 25 publications

References 39 publications

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

Naturally occurring anti-cancer compounds: shining from Chinese herbal medicine

A Danshensu-Tetramethylpyrazine Conjugate DT-010 Overcomes Multidrug Resistance in Human Breast Cancer

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