Anti-cancer effects of thymoquinone in mouse neuroblastoma (Neuro-2a) cells through caspase-3 activation with down-regulation of XIAP

Paramasivam, Arumugam; Shanmugam, Sambantham; Shabnam, Jawahar; Raghunandhakumar, Subramanian; Balakrishnan, Anandan; Rose, Rajiv; Priyadharsini, J. Vijayashree; Jayaraman, G.

doi:10.1016/j.toxlet.2012.06.011

Cited by 69 publications

(32 citation statements)

References 46 publications

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“…Alhosin et al (2010) demonstrated that thymoquinone successfully inhibited proliferation (IC 50 = 24.2 μM after 24 h vs. 23.3 μM after 48 h) and viability (IC 50 = 24.3 μM after 24 h vs. 23.1 μM after 48 h) in p53-defected Jurkat lymphoblastic leukemia cells in a dose- and time-dependent manner. Thymoquinone was also cytotoxic to neuroblastoma Neuro-2a cells in a dose-and time-dependent manner (IC 50 = 40 and 36 μM after 24 and 48 h treatment; Paramasivam et al, 2012). Racoma et al (2013) described that thymoquinone was cytotoxic to several glioblastoma cell lines with Gli36ΔEGFR as the most susceptible one (IC 50 = 2.4 μM).…”

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

“…The bioactive properties of N. sativa are usually associated with the content in thymoquinone (Figure 4, 1) (Agbaria et al, 2015) that have been widely described as anti-inflammatory, antioxidant and anti-neoplasic (Paramasivam et al, 2012; Raghunandhakumar et al, 2013; Agbaria et al, 2015). In fact, the strongest anticancer activity was achieved in seed oils heated between 50 and 150°C with the highest content of thymoquinone (Agbaria et al, 2015).…”

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

“…In addition, it activated caspases-3 and -9 with concomitant increase in PARP, while decreasing the protein levels of XIAP, a selective inhibitor for caspases-3, -7, and -9. All of them are signals that lead to cell death by apoptosis (Paramasivam et al, 2012). By contrast, the compound induced cell death by autophagy inhibition in glioblastoma cells lines (Racoma et al, 2013).…”

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

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North African Medicinal Plants Traditionally Used in Cancer Therapy

et al. 2017

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Background: Cancer is a major cause of mortality worldwide with increasing numbers by the years. In North Africa, the number of cancer patients is alarming. Also shocking is that a huge number of cancer patients only have access to traditional medicines due to several factors, e.g., economic difficulties. In fact, medicinal plants are widely used for the treatment of several pathologies, including cancer. Truthfully, herbalists and botanists in North African countries prescribe several plants for cancer treatment. Despite the popularity and the potential of medicinal plants for the treatment of cancer, scientific evidence on their anticancer effects are still scarce for most of the described plants.Objective: Bearing in mind the lack of comprehensive and systematic studies, the aim of this review is to give an overview of studies, namely ethnobotanical surveys and experimental evidence of anticancer effects regarding medicinal plants used in North Africa for cancer therapy.Method: The research was conducted on several popular search engines including PubMed, Science Direct, Scopus and Web of Science. The research focused primarily on English written papers published between the years 2000 and 2016.Results: This review on plants traditionally used by herbalists in North Africa highlights that Morocco and Algeria are the countries with most surveys on the use of medicinal plants in folk medicine. Among the plethora of plants used, Nigella sativa and Trigonella foenum-graecum are the most referred ones by herbalists for the treatment of cancer. Moreover, a plethora of scientific evidence qualifies them as candidates for further drug development. Furthermore, we report on the underlying cellular and molecular mechanisms.Conclusion: Overall, this review highlights the therapeutic potential of some medicinal plants as anticancer agents. The North African flora offers a rich source of medicinal plants for a wide array of diseases, including cancer. The elucidation of their modes of action represents an indispensable condition for the rational development of new drugs for cancer treatment. Furthermore, testing the anticancer activity in vivo and in clinical trials are warranted to explore the full therapeutic potential of North African plants for cancer therapy.

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Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

Section: Scientific Evidence and Mechanisms Of Actionmentioning

confidence: 99%

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North African Medicinal Plants Traditionally Used in Cancer Therapy

et al. 2017

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“…Jayakumar and collaborators [38, 42] demonstrated that thymol is cytotoxic against HepG2 human hepatoma cells, colonic Caco-2 cells, and K562 cells, via a mechanism that may be related to antioxidant activity and not associated with a DNA-damaging effect. The effects of thymol on murine B16-F10 melanoma cells were tested by Paramasivam and collaborators [45], and thymol exhibited cytotoxicity with an IC 50 value of 88.5 μ g/mL. Thymol cytotoxicity was reduced by addition of vitamin C and vitamin E. Radical scavengers (butylated hydroxytoluene and butylated hydroxyanisole) were able to significantly recover cell viability.…”

Section: Introductionmentioning

confidence: 99%

Antitumor Activity of Monoterpenes Found in Essential Oils

Sobral

Xavier

Lima

et al. 2014

The Scientific World Journal

215

142

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Cancer is a complex genetic disease that is a major public health problem worldwide, accounting for about 7 million deaths each year. Many anticancer drugs currently used clinically have been isolated from plant species or are based on such substances. Accumulating data has revealed anticancer activity in plant-derived monoterpenes. In this review the antitumor activity of 37 monoterpenes found in essential oils is discussed. Chemical structures, experimental models, and mechanisms of action for bioactive substances are presented.

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“…Thymoquinone is an active constituent of the plant Nigella sativa , and has a potential role in the treatment of cancer. Previous researchers have reported that thymoquinone reduces the proliferation of lung cancer, breast cancer, colon cancer, melanoma,12,13 liver cancer,14 neuroblastoma,15 and oral cancer cells 16…”

Section: Introductionmentioning

confidence: 99%

Potential implications of GRP58 expression and susceptibility of cervical cancer to cisplatin and thymoquinone-based therapy

Hafiza¹,

Latifah²

2014

OTT

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A new therapeutic approach of looking at the expression of glucose-regulated protein (GRP) 58 as an indication of cisplatin sensitivity may eradicate fruitless treatment and side effects in patients with cervical cancer. Thymoquinone, the bioactive compound in Nigella sativa, has been reported to have an antiproliferative effect on cervical cancer cells. This study compared the cytotoxic effects of cisplatin, a drug commonly used in the treatment of cervical cancer, and thymoquinone in cervical cancer (HeLa and SiHa) cell lines by 3-(4,5-Dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and measured GRP58 expression in the cells by quantitative real-time polymerase chain reaction and Western blotting. Cisplatin had higher antiproliferative activity towards the cervical cancer cell lines than thymoquinone in a dose-dependent and time-dependent manner. However, cisplatin was more toxic to normal 3T3 and Vero cell lines than thymoquinone. The half maximal inhibitory concentration (IC50) of cisplatin in HeLa and SiHa cells at 72 hours was 13.3±2.52 μM and 19.5±2.12 μM, respectively. Meanwhile, the IC50 of thymoquinone in HeLa and SiHa cells was 29.57±5.81 μM and 23.41±1.51 μM, respectively (P<0.05). A significant correlation was found between the cytotoxicity of cisplatin and expression of GRP58, but this relationship was not significant for thymoquinone. Therefore, the response of cervical cancer cells to cisplatin can be predicted on the basis of GRP58 expression.

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Anti-cancer effects of thymoquinone in mouse neuroblastoma (Neuro-2a) cells through caspase-3 activation with down-regulation of XIAP

Cited by 69 publications

References 46 publications

North African Medicinal Plants Traditionally Used in Cancer Therapy

North African Medicinal Plants Traditionally Used in Cancer Therapy

Antitumor Activity of Monoterpenes Found in Essential Oils

Potential implications of GRP58 expression and susceptibility of cervical cancer to cisplatin and thymoquinone-based therapy

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