Anti-cancer Evaluation of Depsides Isolated from Indonesian Folious Lichens: Physcia millegrana, Parmelia dilatata and Parmelia aurulenta

Nugraha, Ari Satia; Laksono, Tinton Agung; Firli, Lilla Nur; Putri, Chintya Permata Zahky Sukrisno; Pratoko, Dwi Koko; Zulfikar, Zulfikar; Untari, Ludmilla Fitri; Wongso, Hendris; Lambert, Jacob M; Dillon, Carolyn T.; Keller, P. A.

doi:10.3390/biom10101420

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…The +(−) usnic acid present in abundance (13.3%) in UC2A is known to induce cytotoxicity in multiple cancer cell lines [ 14 ]. Atraric acid isolated from lichens, including Physcia and Parmelia sp., has shown cytotoxic potential against HepG2, A549 and HL-60 cells [ 27 ]. Barbatic acid is also a unique metabolite of Usnea sp.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

Kumari

Kamat

Singh

et al. 2023

Plants

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The Western Ghats, India, is a hotspot for lichen diversity. However, the pharmacological importance of lichen-associated metabolites remains untapped. This study aimed to evaluate the cytotoxic potential of lichens of this region. For this, sixteen macrolichens were collected and identified from two locations in the Western Ghats. The acetone extract of Usnea cornuta (UC2A) showed significant cytotoxicity towards multiple human cancer cell lines. Interestingly, co-treatment with chloroquine (CQ), an autophagy inhibitor, increased the cytotoxic potential of the UC2A extract. A gas chromatography mass spectrometry (GCMS) study revealed usnic acid (UA), atraric acid and barbatic acid as the dominant cytotoxic compounds in the UC2A extract. Further, UA was purified and identified from the UC2A extract and evaluated for cytotoxicity in HeLa cells. The monodansyl cadaverine and mitotracker red double staining revealed the autophagy-inducing activities of UA, and the inhibition of autophagy was confirmed via CQ treatment. Autophagy inhibition increased the cytotoxicity of UA by 12–16% in a concentration-dependent manner. It also increased lipid peroxidation, ROS levels and mitochondrial depolarization and decreased glutathione availability. A decrease in zeta potential and a 40% increase in caspase 3/7 activity were also noted after CQ treatment of UA-treated cells. Thus, cytotoxicity of UA can be increased by inhibiting autophagy.

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Section: Resultsmentioning

confidence: 99%

Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

Kumari

Kamat

Singh

et al. 2023

Plants

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“…Cancer is one of the significant public health concerns in global societies with high unmet medical needs (Wongso et al 2013;Nugraha et al 2020). The typical treatment strategies for cancer consist of chemotherapy, surgery, radiotherapy, bone marrow transplants, or combination therapies between those treatments.…”

Section: Anticancer Activities Of Bcmentioning

confidence: 99%

Pharmacological activities and potential use of bovine colostrum for peptide-based radiopharmaceuticals: A review

Kusumaningrum¹,

Widyasari²,

Sriyani³

et al. 2021

PHAR

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Bovine colostrum (BC) is the initial milk produced by cows after giving birth. It has been used to treat human diseases, such as infections, inflammations, and cancers. Accumulating evidence suggests that bovine lactoferrin and bovine antibodies seem to be the most important bioactive constituents in BC. Thus, BC has also been reviewed for its potential to deliver short-term protection against coronavirus disease 2019 (COVID-19). In addition, it can potentially be explored as a precursor for peptide-based radiopharmaceuticals. To date, several bioactive peptides have been isolated from BC, including casocidin-1, casecidin 15 and 17, isracidin, caseicin A, B, and C. Like other peptides, bioactive peptides derived from BC could be used as a valuable precursor for radiopharmaceuticals either for diagnosis or therapy purposes. This review provides bovine colostrum’s biological activities and a perspective on the potential use of peptides from BC for developing radiopharmaceuticals in nuclear medicine.

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“…Among the metabolites widely known to be produced by lichens, depsides are a class of polyphenols structurally featuring two or more monocyclic aromatic moieties linked by an ester bond. This class of compounds has previously shown antioxidant activity [ 20 , 21 ], antiproliferative activity on cancer cells [ 22 , 23 ], antibiofilm activity [ 24 ], antimicrobial activities on both bacteria and fungi [ 25 , 26 ], and some other relevant bioactivities [ 27 ]. Compounds belonging to this structural class were already detected in the preliminary chemical analysis of the gypsiferous lichen extracts.…”

Section: Introductionmentioning

confidence: 99%

Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides

Fernández-Pastor,

González-Menéndez,

Martínez Andrade

et al. 2023

JoF

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In a survey to evaluate the potential of lichens associated with gypsum areas as sources of new antifungal metabolites, six species of lichens were collected in the gypsum outcrops of the Sorbas Desert (Diploschistes ocellatus and Seirophora lacunosa) and the Tabernas Desert (Cladonia foliacea, Acarospora placodiformis, Squamarina lentigera and Xanthoparmelia pokornyi) in southern Spain. Raw lichen acetone extracts were tested against a panel of seven phytopathogenic fungi, including Botrytis cinerea, Colletotrichum acutatum, Fusarium oxysporum f.sp cubense TR4, Fusarium ploriferaum, Magnaporthe grisea, Verticillium dahliae and Zymoseptoria tritici. Active extracts of Cladonia foliacea, Xanthoparmelia pokornyi and Squamarina lentigera were analyzed by HPLC-MS/MS and Molecular Networking to identify possible metabolites responsible for the antifungal activity. A total of ten depside-like metabolites were identified by MS/MS dereplication and NMR experiments, of which one was a new derivative of fumaroprotocetraric acid. The compounds without previously described biological activity were purified and tested against the panel of fungal phytopathogens. Herein, the antifungal activity against fungal phytopathogens of 4′-O-methylpaludosic acid, divaricatic acid and stenosporic acid is reported for the first time. Stenosporic and divaricatic acids displayed a broad antifungal spectrum against seven relevant fungal phytopathogens in a micromolar range, including the extremely resistant fungus F. oxysporum f. sp. cubense Tropical Race 4 (TR4). 4′-O-methylpaludosic acid exhibited specific antifungal activity against the wheat pathogen Z. tritici, with an IC50 of 38.87 µg/mL (87.1 µM) in the absorbance-based assay and 24.88 µg/mL (55.52 µM) in the fluorescence-based assay.

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Anti-cancer Evaluation of Depsides Isolated from Indonesian Folious Lichens: Physcia millegrana, Parmelia dilatata and Parmelia aurulenta

Cited by 11 publications

References 24 publications

Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

Pharmacological activities and potential use of bovine colostrum for peptide-based radiopharmaceuticals: A review

Xerophytic Lichens from Gypsiferous Outcrops of Arid Areas of Andalusia as a Source of Anti-Phytopathogenic Depsides

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