Anti-caries effect of resin infiltrant modified by quaternary ammonium monomers

Yu, Jinzhao; Huang, Xiaoyu; Han, Qi; Zhou, Wen; Liang, Jingou; Xu, Hockin H.K.; Ren, Biao; Peng, Xian; Weir, Michael D.; Li, Mingyun; Cheng, Lei

doi:10.1016/j.jdent.2020.103355

Cited by 26 publications

(23 citation statements)

References 78 publications

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“…The antimicrobial mechanism of QAMs was considered "contact killing", since the positive quaternary amine charge could destroy the bacterial membrane which was negatively charged [89]. Compared with the releasing materials, QAMs can be copolymerized with the resin matrix [90] and fixed in the polymer network with a prolonged antimicrobial activity. Thomé et al [91] found that the 12-methacryloyloxydodecylpyridinium bromide (MDPB)-containing composite inhibited the progression of artificial secondary root caries by Streptococcus mutans.…”

Section: Antimicrobial Materialsmentioning

confidence: 99%

Dental Restorative Materials for Elderly Populations

et al. 2021

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The incidence of dental caries, especially root caries, has risen in elderly populations in recent years. Specialized restorative materials are needed due to the specific site of root caries and the age-related changes in general and oral health in the elderly. Unfortunately, the restorative materials commonly used clinically cannot fully meet the requirements in this population. Specifically, the antibacterial, adhesive, remineralization, mechanical, and anti-aging properties of the materials need to be significantly improved for dental caries in the elderly. This review mainly discusses the strengths and weaknesses of currently available materials, including amalgam, glass ionomer cement, and light-cured composite resin, for root caries. It also reviews the studies on novel anti-caries materials divided into three groups, antimicrobial, remineralization, and self-healing materials, and explores their potential in the clinical use for caries in the elderly. Therefore, specific restorative materials for caries in the elderly, especially for root caries, need to be further developed and applied in clinical practice.

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Section: Antimicrobial Materialsmentioning

confidence: 99%

Dental Restorative Materials for Elderly Populations

et al. 2021

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“…CHX as a broadspectrum antimicrobial agent can effectively inhibit most of oral microbes but with poor selectivity, which might impose adverse effects on the oral microbial ecology after long-term use (Tokajuk et al, 2017;Sakaue et al, 2018). A multispecies biofilm model consisting of S. mutans, S. sanguinis, and S. gordonii was reported previously that could be used to evaluate the ecological impact of the tested compounds on oral biofilms (Zhang et al, 2019;Yu et al, 2020). In the current study, although S. mutans was less sensitive to LCG-N25 (MIC = 0.5 µg/mL) relative to the other 2 streptococci (MICs = 0.125 µg/mL) in the planktonic culture, data obtained from FISH showed that treatment with LCG-N25 led to a decreased proportion of S. mutans and an elevation of other commensal streptococci within mixed biofilms.…”

Section: Discussionmentioning

confidence: 99%

A Novel Small Molecule, LCG-N25, Inhibits Oral Streptococcal Biofilm

Lyu

Zhang

et al. 2021

Front. Microbiol.

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Dental caries is a chronic oral infectious disease caused by cariogenic biofilm adhered on the tooth surface. Our previous study demonstrated that a repurposed natural compound napabucasin (NAP) showed good antimicrobial activity against oral streptococcal biofilms. The current study designed a novel small molecule, namely LCG-N25, using NAP as a lead compound, and aimed to investigate its potential as an antimicrobial agent in the control of dental caries. LCG-N25 was designed and synthesized with reference to the structure of NAP. The minimal inhibitory concentrations and the minimal bactericidal concentrations of LCG-N25 against Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were evaluated by microdilution method. The antimicrobial activity of LCG-N25 was further evaluated by crystal violet staining, colony forming units counting, biofilm metabolism assay, dead/live fluorescent staining, and scanning electron microscopy. The effect of LCG-N25 on the extracellular polysaccharides of biofilms was determined by both anthrone-sulfuric acid method and fluorescent staining. The microbial composition of streptococcal biofilms after LCG-N25 treatment was further visualized and quantified by fluorescence in situ hybridization. Besides, the cytotoxicity of LCG-N25 was evaluated by Cell Counting Kit-8 assay, and repeated exposure of S. mutans to LCG-N25 treatment was performed to assess if this novel compound could induce drug resistance of this cariogenic bacterium. We found that LCG-N25 exhibited a good antibacterial activity, low-cytotoxicity, and did not induce drug resistance of cariogenic S. mutans. These findings suggest that LCG-N25 may represent a promising antimicrobial agent that can be used as an adjuvant to the management of dental caries.

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“…In addition, some studies pointed out resin infiltrant lacks persistent antibacterial effects and cannot inhibit bacterial growth ( Tawakoli, Attin & Mohn, 2016 ). Therefore, some scholars add antibacterial substances, such as silver nanoparticles (AgNP) ( Kielbassa et al, 2020 ), quaternary ammonium methacrylate ( Yu et al, 2020 ) to provide the effect of the antibacterial resin penetrant. Our research has not made further discussion on this.…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of resin infiltrant on resistance of sound enamel surfaces in permanent teeth to demineralization

Yang

Huang

et al. 2021

PeerJ

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Objective To investigate the effect of resin infiltrant on resistance of sound permanent enamel surfaces to demineralization. Method Eighty healthy premolars were sectioned to obtain enamel blocks from the buccal surface. Specimens with baseline surface microhardness values of 320–370 were selected. The experimental group were treated with resin infiltrant, while the control group was not. Specimens from each group were artificially demineralized and the surface microhardness values were measured again. Confocal laser scanning microscopy was used to measure the depth of demineralization and detect the penetration ability of the resin infiltrant. The specimens were subjected to a simulated toothbrushing abrasion test. Scanning electron microscopy was used to observe changes in the surface morphology of specimens after each of these procedures. Results No significant differences between the experimental and control groups were observed in the baseline microhardness values or in the experimental group after resin infiltration compared with the baseline conditions. After artificial demineralization, the microhardness value in the control group was significantly lower than that in the experimental group (266.0 (±34.5) compared with 304.0 (±13.0), P = 0.017). Confocal laser scanning microscopy results showed that the demineralization depth in the control group was significantly deeper than that in the experimental group (97.9 (±22.8) µm vs. 50.4 (±14.3) µm, P < 0.001), and that resin infiltrant completely penetrated the acid-etched demineralized area of the tooth enamel with a mean penetration depth of 31.6 (±9.0) µm. Scanning electron microscopy showed that the surface morphology was more uniform and smoother after simulated toothbrushing. The enamel surface structure was more severely destroyed in the control group after artificial demineralization compared with that of the experimental group. Conclusion Resin infiltrant can completely penetrate an acid-etched demineralized enamel area and improve resistance of sound enamel surfaces to demineralization. Our findings provide an experimental basis for preventive application of resin infiltrant to sound enamel surfaces to protect tooth enamel against demineralization.

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Anti-caries effect of resin infiltrant modified by quaternary ammonium monomers

Cited by 26 publications

References 78 publications

Dental Restorative Materials for Elderly Populations

Dental Restorative Materials for Elderly Populations

A Novel Small Molecule, LCG-N25, Inhibits Oral Streptococcal Biofilm

In vitro effect of resin infiltrant on resistance of sound enamel surfaces in permanent teeth to demineralization

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