ANti‐CD3 antibody‐treated mice: In Vivo induction of cytolytic activity and tnf production by lung leukocytes

Abstract

Anti-CD3 antibody induced non-MHC-restricted cytolytic activity in murine spleen cells within 4 hr of incubation in vitro and within 8 hr in vivo after intraperitoneal injection. Interstitial lung leukocytes acquired the capacity to kill both NK-sensitive and -resistant targets within 24 hr after anti-CD3 injection. In vivo-stimulated spleen cells produced significantly more IL-2 and TNF than unstimulated cells. When these cells were restimulated with anti-CD3 in vitro, a potentiation of TNF production was seen. Interstitial lung leukocytes doubled their TNF production ex vivo after anti-CD3 injection, and the suppressed TNF production which was seen in lung cells from tumor-bearing animals was restored after anti-CD3 stimulation. The TNF production by alveolar macrophages was augmented 5-fold 48 hr after injection of antibody. Anti-CD3 antibody also induced significant accumulation of both TNF alpha and TNF beta mRNA in spleen and lung leukocytes. Pre-treatment with anti-TNF antibodies, both in vitro and in vivo, did not eliminate the cytolytic activation of lung and spleen cells. Our data indicate that anti-CD3 antibodies can induce rapid activation of both cytolytic activity and cytokine production in lung lymphocytes and macrophages.

Effector and Activated T Cells Induce Preterm Labor and Birth That Is Prevented by Treatment with Progesterone

“…In vivo T cell activation induces preterm birth by causing hypothermia, bradycardia, and cervical dilation Next, we investigated the mechanisms whereby effector/activated T cells could induce preterm labor and birth by using a murine model. In vivo T cell activation is achieved by the administration of an anti-CD3 Ab that induces a cytokine-related syndrome leading to hypothermia (90)(91)(92)(93)(94); therefore, we used this strategy in pregnant mice. Dams injected with anti-CD3ε delivered preterm (Fig.…”