Anti-Colon Cancer Activity of Dietary Phytochemical Soyasaponin I and the Induction of Metabolic Shifts in HCT116

Abstract

Dietary phytochemicals play an important role in the prevention and treatment of colon cancer. It is reported that group B of soyasaponin, derived from dietary pulses, has anti-colonic effects on some colon cancer cell lines. However, it is uncertain which specific soybean saponins play a role. In our study, as one of the group B soyasaponin, the anti-colon cancer activity of soyasaponins I (SsI) was screened, and we found that it had the inhibitory effect of proliferation on colon cancer cell lines HCT116 (IC50 = 161.4 μM) and LoVo (IC50 = 180.5 μM), but no effect on HT29 between 0–200 μM. Then, nine potential targets of SsI on colon cancer were obtained by network pharmacology analysis. A total of 45 differential metabolites were identified by metabolomics analysis, and the KEGG pathway was mainly enriched in the pathways related to the absorption and metabolism of amino acids. Finally, molecular docking analysis predicted that SsI might dock with the protein of DNMT1, ERK1. The results indicated that the effect of SsI on HCT116 might be exerted by influencing amino acid metabolism and the estrogen signaling pathway. This study may provide the possibility for the application of SsI against colon cancer.

Effects of ageing and salt concentrations on metabolite profiles and their correlation with consumer acceptance in Korean traditional doenjang (fermented soybean paste)

“…Therefore, to product more bioactive soyasaponins, the ageing period with appropriate levels of salt content is necessary. Low‐salt doenjang could reduce salt consumption; however, it also diminishes the consumption of soyasaponins, key bioactive compounds in soy foods for anti‐cancer (Xia et al ., 2022), neuroprotection (Hong t al., 2013) and anti‐obesity (Kamo et al ., 2018) materials. The aglycone form of isoflavonoids, such as daidzein, glycitein and genistein, was detected in doenjang samples (Fig 3n‐p).…”

Targeting NF‐κB/COX‐2 signaling by soyasaponin I alleviates diclofenac‐induced gastric ulceration in male albino rats

“…Soyasaponins include four principal groups (A, B, E, and DDMP) with various members. Soyasaponin I (also called soyasaponin Bb) belongs to group B 15 and could be extracted from the whole parts of the Desmodium styracifolium plant 7 …”

“…Soyasaponins include four principal groups (A, B, E, and DDMP) with various members. Soyasaponin I (also called soyasaponin Bb) belongs to group B 15 and could be extracted from the whole parts of the Desmodium styracifolium plant. 7 Although saponins have displayed anti-inflammatory effects in different animal models, 16,17 their impact on a rodent model of experimentally induced gastric ulcer has not yet been examined.…”

Molecular mechanisms of bitterness and astringency in the oral cavity induced by soyasaponin

“…These are natural surfactants with potential applications as emulsifiers and foaming agents in food products [19]. In addition, Ssa's exhibit various biological activities, including anticancer [20], antioxidant [21], and hypoglycemic [22] activities.…”