Anti-Cryptosporidium efficacy of Citrus sinensis peel extract: Histopathological and ultrastructural experimental study

Wahab, Wegdan M. Abd El; Shaapan, Raafat M.; El-Naggar, El-Moataz Bellah; Ahmed, Marwa Mostafa; Owis, Asmaa I.; Ali, Mona Ibrahim

doi:10.1016/j.exppara.2022.108412

Cited by 3 publications

(2 citation statements)

References 28 publications

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“…C. sinensis extract at a dose of 30 mg/kg compared to nitazoxanide, as a standard drug, led to a considerable decline in oocyst count. In addition, amelioration of pathological lesions was observed in treated mice ( Abd El Wahab et al, 2022 ).…”

Section: Resultsmentioning

confidence: 97%

Herbal-based compounds: A review on treatments of cryptosporidiosis

Namazi,

Razavi

2024

International Journal for Parasitology: Drugs and Drug Resistan

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Section: Resultsmentioning

confidence: 97%

Herbal-based compounds: A review on treatments of cryptosporidiosis

Namazi,

Razavi

2024

International Journal for Parasitology: Drugs and Drug Resistan

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“…Several attempts were made to evaluate plant and medicinal herb extracts for the treatment and prevention of cryptosporidosis in vivo and in vitro, including the use of curcumin (Asadpour et al, 2018), olive ethanolic extract (Teichmann et al, 2016), Citrus sinensis peel extract (Abd El Wahab et al, 2022), and Herniaria hemistemon aerial extract (Ghareeb et al, 2023). Additionally, a number of investigates have been carried out to evaluate the impact of A. sativum extracts on cryptosporidiosis (Abouel‐Nour et al, 2016; EL‐Shewehy et al, 2022; Farid et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Hamdy,

Ismail,

El‐Askary

et al. 2024

Microscopy Res & Technique

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Cryptosporidiosis is a global health problem threats life of immunocompromised patients. Allium sativum (A. sativum) is one of the therapeutic options for cryptosporidiosis. This study develops green synthesized ZnO‐NPs based on A. sativum extract, and assesses its therapeutic application in treating experimental cryptosporidiosis in immunosuppressed mice. FTIR, scanning electron microscopy, and zeta analyzer were used for characterization of bio ZnO‐NPs. The morphology of prepared materials appeared as sponge with many pores on the whole surface that allows the feasibility of bio ZnO‐NPs for different biological activities. Its structural analysis was highly stabilized with negative charge surface which indicated for well distribution into the parasite matrix. Twenty‐five immunosuppressed Cryptosporidium parvum infected mice, classified into 5 groups were sacrificed at 21th day after infection with evaluation of parasitological, histopathological, oxidative, and proinflammatory biomarkers. Treated mice groups with 50 and 100 mg/kg of AS/ZnO‐NPs showed a highly significant decline (79.9% and 83.23%, respectively) in the total number of expelled oocysts. Both doses revealed actual amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. They also significantly produced an increase in GSH values and improved the changes in NO and MDA levels, and showed high anti‐inflammatory properties. This study is the first to report green synthesis of ZnO/A. sativum nano‐composite as an effective therapy in treating cryptosporidiosis which gave better results than using A. sativum alone. It provides an economical and environment‐friendly approach towards novel delivery synthesis for antiparasitic applications.Research Highlights Green synthesis of ZnO‐NPs was developed using A. sativum extract. The morphology of prepared ZnO‐NPs appeared as sponge with many pores on SEM The study evaluates its therapeutic efficacy against murine cryptosporidiosis The green synthesized ZnO‐NPs significantly reduced percent of oocyst shedding, improved the pathological changes, and showed high antioxidant and anti‐inflammatory potentials.

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Newly fabricated zinc oxide nanoparticles loaded materials for therapeutic nano delivery in experimental cryptosporidiosis

Hamdy,

Ismail,

El-Askary

et al. 2023

Sci Rep

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Cryptosporidiosis is a global health problem that threatens the lives of immunocompromised patients. This study targets to fabricate and investigate the efficiency of zinc oxide nanoparticles (ZnO-NPs), nitazoxanide (NTZ)-loaded ZnO-NPs, and Allium sativum (A. sativum)-loaded ZnO-NPs in treating cryptosporidiosis. Further FTIR, SEM, XRD, and zeta analysis were used for the characterization of ZnO-NPs and loaded materials. The morphology of loaded materials for ZnO-NPs changed into wrapped layers and well-distributed homogenous particles, which had a direct effect on the oocyst wall. The charge surface of all particles had a negative sign, which indicated well distribution into the parasite matrix. For anti-cryptosporidiosis efficiency, thirty immunosuppressed Cryptosporidium parvum-infected mice, classified into six groups, were sacrificed on the 21st day after infection with an evaluation of parasitological, histopathological, and oxidative markers. It was detected that the highest reduction percent of Cryptosporidium oocyst shedding was (81.5%) in NTZ, followed by (71.1%) in A. sativum-loaded ZnO-NPs-treated groups. Also, treatment with A. sativum and NTZ-loaded ZnO-NPs revealed remarkable amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. Furthermore, they significantly produced an increase in GSH values and improved the changes in NO and MDA levels. In conclusion, this study is the first to report ZnO-NPs as an effective therapy for treating cryptosporidiosis, especially when combined with other treatments that enhance their antioxidant activity. It provides an economical and environment-friendly approach to novel delivery synthesis for antiparasitic applications.

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Anti-Cryptosporidium efficacy of Citrus sinensis peel extract: Histopathological and ultrastructural experimental study

Cited by 3 publications

References 28 publications

Herbal-based compounds: A review on treatments of cryptosporidiosis

Herbal-based compounds: A review on treatments of cryptosporidiosis

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Newly fabricated zinc oxide nanoparticles loaded materials for therapeutic nano delivery in experimental cryptosporidiosis

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