Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus

McPhee, Sarah; Hodges, Lynn D.; Wright, Paul; Wynne, Paul; Kalafatis, Nicolette; Harney, D.W.; Macrides, Theodore A.

doi:10.1016/j.cbpb.2006.11.001

Cited by 56 publications

(59 citation statements)

References 34 publications

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“…Because the common components for these fractions are apparently fatty acids, we focused on the further analysis of the fatty acid components. This estimation agrees with Macrides and coworkers' report that the free fatty acid fraction, and to a lesser extent the triglyceride fraction, were the only lipid classes to exhibit strong inhibition of COX isoforms (8). However, a precise separation of the free fatty acid fraction is usually difficult for minor fatty acids in the presence of an abundance of PUFAs.…”

Section: Resultssupporting

confidence: 91%

“…The anti-inflammatory effect of Lyprinol was previously examined in various assay systems, including a rat model of adjuvant-induced arthritis (9) and a mouse model of bowel disease (10) in vivo and leukotriene biosynthesis by human polymorphonuclear leukocytes (7) and cyclooxygenase inhibition (8) in vitro. Among them, we used the rat model with adjuvant-induced arthritis, because the potent anti-inflammatory activity of Lyprinol had previously been assessed in this model after oral administration.…”

Section: Resultsmentioning

confidence: 99%

“…The development of a natural remedy for the management of arthritis that will not only replace nonsteroidal anti-inflammatories (NSAIDs) as the standard treatment for all types of arthritis but also treat the condition successfully, without any known side effects, is being eagerly pursued (1). Therefore, numerous studies have been published on the lipid composition of the extract (2-4), its complex mode of action (5)(6)(7)(8), activity in animal models (9,10), and efficacy in controlling osteoarthritis (11,12) and moderate asthma in patients (13). However, the active component from the mussel is a lipid rich in polyunsaturated fatty acids (PUFAs) and is therefore prone to activation of degrading enzymes including phospholipases and lipoxygenases (14,15) after cell damage, unless it is stored and transported properly.…”

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confidence: 99%

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Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

Wakimoto

Kondo

Nii

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

104

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A lipid extract of Perna canaliculus (New Zealand green-lipped mussel) has reportedly displayed anti-inflammatory effects in animal models and in human controlled studies. However, the anti-inflammatory lipid components have not been investigated in detail due to the instability of the lipid extract, which has made the identification of the distinct active components a formidable task. Considering the instability of the active component, we carefully fractionated a lipid extract of Perna canaliculus (Lyprinol) and detected furan fatty acids (F-acids). These naturally but rarely detected fatty acids show potent radical-scavenging ability and are essential constituents of plants and algae. Based on these data, it has been proposed that F-acids could be potential antioxidants, which may contribute to the protective properties of fish and fish oil diets against chronic inflammatory diseases. However, to date, in vivo data to support the hypothesis have not been obtained, presumably due to the limited availability of F-acids. To confirm the in vivo anti-inflammatory effect of F-acids in comparison with that of eicosapentaenoic acid (EPA), we developed a semisynthetic preparation and examined its anti-inflammatory activity in a rat model of adjuvant-induced arthritis. Indeed, the F-acid ethyl ester exhibited more potent anti-inflammatory activity than that of the EPA ethyl ester. We report on the in vivo activity of F-acids, confirming that the lipid extract of the green-lipped mussel includes an unstable fatty acid that is more effective than EPA.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

Wakimoto

Kondo

Nii

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

104

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“…50 % n-3 PUFA including up to 15 % EPA and 20 % DHA (12) . As with oily fish, treatment with GLM oil at a moderate dose significantly reduces the pro-inflammatory response both in vitro (22) and in vivo (23) ; therefore, the replacement of red meat with GLM within the diet may also provide cardioprotective properties. In summary, we have shown that GLM digestate can enhance non-haem Fe uptake in Caco-2 cells to a similar degree to that of beef.…”

Section: Discussionmentioning

confidence: 99%

New Zealand green-lipped mussels (Perna canaliculus) enhance non-haem iron absorptionin vitro

et al. 2012

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Fe bioavailability can be manipulated by the nutritional composition of a meal. Ascorbic acid and unidentified components of meat, fish and poultry, but particularly beef, all appear to enhance the absorption of non-haem Fe. The aim of the present study is to identify whether extracts of green-lipped mussels (GLM; Perna canaliculus) enhance non-haem Fe absorption in Caco-2 cells and to compare the effect with that of beef. Raw GLM and raw beef homogenates were digested in vitro with pepsin at pH 2, and pancreatin and bile salts at pH 7. Tracer 55 Fe was used to measure cellular Fe uptake. Ascorbic acid was used as a positive control and egg albumin, exposed to the same in vitro digestion process, was used as a negative control. Caco-2 cell monolayers were incubated with treatments for 60 min. All values were standardised per mg of GLM, egg albumin, beef or ascorbic acid. The results showed that ascorbic acid enhanced nonhaem Fe absorption to the highest degree. Beef and GLM digestates both significantly enhanced Fe absorption compared with egg albumin. In conclusion, GLM digestate significantly enhances non-haem Fe uptake in Caco-2 cells with a similar magnitude to that of beef.Key words: Iron absorption: Mussels: Caco-2: Meat factor Red meat, pork, poultry and fish all significantly enhance Fe absorption when consumed as part of a vegetable-based meal in human subjects (1) . Further investigation into the bioactive components of meat, poultry and fish factor suggests that cysteine-rich myofibrils (2) , glycosaminoglycans (3) and L-a-glycerophosphocholine (4) could be responsible for this enhancing effect, either individually or in combination (5) . Other dietary factors including PUFA (6) and non-digestible soluble carbohydrates including inulin and fructooligosaccharides (7) may also increase non-haem Fe absorption; however, the magnitude of enhancement and the mechanism remains elusive.Of all meat sources, beef has been repeatedly reported to promote Fe absorption to the highest degree in human subjects (1,4,8) . However, addition of SFA to the diet via increased meat consumption or the replacement of dietary PUFA with SFA is strongly associated with negative cardiovascular outcomes, while the reverse is cardio-protective (9) . For this reason, promoting red meat consumption in order to improve Fe status at a population level may be problematic, and the identification of an alternative meat, poultry and fish factor source rich in PUFA or low in SFA is warranted. The effect of oily fish on Fe uptake has been investigated in vivo; however, protocol inconsistencies have led to contradicting results (6,10,11) .There has been little investigation into the effect of bivalve molluscs on non-haem Fe absorption both in vitro and in vivo. New Zealand green-lipped mussels (GLM, Perna canaliculus) are rich in both haem and non-haem Fe, myofibrillar proteins, low-molecular-weight aminoglycans and n-3 PUFA (12) , and may therefore provide an alternative source of meat factor. The aim of the present study is to inve...

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“…Based on our best knowledge, a publication on antiinflammatory and cytotoxic compounds from horse mussel M. modiolus is not available so far. McPhee et al (2007) reported that sterol isolated from the New Zealand green-lipped mussel Perna canaliculus exhibited anti-cyclooxygenase (COX-2), a lipid metabolising enzyme that catalyses the oxygenation of polyunsaturated fatty acids (PUFA), preferably arachidonic acid, to form the prostanoids, potent cellsignalling molecules associated with the inflammatory processes. Tenikoff et al (2005) reported that desmosterol-containing lyprinol isolated from the New Zealand green-lipped mussel had potential effect in the treatment of inflammatory bowel disease in mice.…”

Section: Purification Of Anti-inflammatory and Anticancer Compoundsmentioning

confidence: 99%

Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture

Wikarta

Kim

2015

Cytotechnology

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The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3b,5a)-(58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3b,5a,22E) (13.0 %). This extract exhibited strong anti-inflammatory activity determined solely through the nitric oxide inhibition assay in a dose-dependant manner with the IC 50 value of 9.6 lg/mL and no cytotoxic effect on the macrophages. Moreover, it also exhibited strong cytotoxicity with the IC 50 values of 21.4, 36.4, and 37.1 lg/mL against AGS, DLD-1, and HeLa cells, respectively. These results indicated that the horse mussel extract might be a functional ingredient in the prevention of inflammation and human cancers.

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Anti-cyclooxygenase effects of lipid extracts from the New Zealand green-lipped mussel, Perna canaliculus

Cited by 56 publications

References 34 publications

Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

Furan fatty acid as an anti-inflammatory component from the green-lipped mussel Perna canaliculus

New Zealand green-lipped mussels (Perna canaliculus) enhance non-haem iron absorptionin vitro

Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture

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