Anti-Diabetic activities of fucosterol fromPelvetia siliquosa

Lee, Yeon Sil; Shin, Kuk Hyun; Kim, Bak-Kwang; Lee, Sang‐Hyun

doi:10.1007/bf02975115

Cited by 124 publications

(76 citation statements)

References 6 publications

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“…Ethyl acetate extract of S. wightii showed the presence of fucosterol, which has already been reported to have antidiabetic properties (Lee et al 2004). Fucosterol is a natural compound, mainly present in marine algae, which is used in the treatment of diabetes by inhibiting glycogen breakdown in the liver.…”

Section: Discussionmentioning

confidence: 99%

“…This may also significantly delay carbohydrate digestion and glucose absorption. The presence of fucosterol, and its antidiabetic, hepatoprotective and antioxidant activity, has been reported in the marine algae Pelvetia (Lee et al 2004). The identified compounds in the extracts of both algal species showed the presence of major saturated and unsaturated fatty acids.…”

Section: Discussionmentioning

confidence: 99%

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Antidiabetic potential of marine algae by inhibiting key metabolic enzymes

Unnikrishnan

Suthindhiran

Jayasri

2015

Frontiers in Life Science

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Two edible seaweeds, Sargassum polycystum and Sargassum wightii, were investigated for their antidiabetic potential using in vitro enzyme inhibitory assays. Among the various extracts, petroleum ether and ethyl acetate extracts of S. wightii showed significant inhibitory effects against α-amylase (IC 50 378.3 μg/ml) and α-glucosidase (IC 50 314.8 μg/ml). Methanol extract of S. wightii showed the highest inhibition against dipeptidyl peptidase-IV (DPP-IV) (IC 50 38.27 μg/ml) and moderate antioxidant activity was observed in acetone extract of S. wightii (44%). Similarly, ethyl acetate extract of S. polycystum showed the highest inhibition against α-amylase (IC 50 438.5 μg/ml) and methanol extract of S. polycystum showed maximum inhibition against α-glucosidase (IC 50 289.7 μg/ml) and DPP-IV (36.94 μg/ml). The antioxidant activity was poor (22%). The extracts were investigated for in vitro cytotoxicity, DNA fragmentation in macrophages and haemolytic activity against erythrocytes, but no notable toxicity was observed with any of the tested extracts. Gas chromatography-mass spectrometry revealed the presence of the antidiabetic compound fucosterol and other major bioactive compounds, giving an insight into the antidiabetic and antioxidant properties of these algae. This study reveals the possible mechanisms of antidiabetic action in vitro, and these two seaweeds may also have an antidiabetic action in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antidiabetic potential of marine algae by inhibiting key metabolic enzymes

Unnikrishnan

Suthindhiran

Jayasri

2015

Frontiers in Life Science

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“…Results of our previous studies indicate that the ether fraction of P. siliquosa exhibits hepatoprotective and glucoselowering effects, and fucosterol isolated from P. siliquosa has antioxidant and antidiabetic activities (Lee et al, 2002(Lee et al, , 2003(Lee et al, , 2004b. Additionally, it has been reported that fucosterol decreases angiotensin-converting enzyme levels, with a reduction in the glucocorticoid receptor in endothelial cells and inhibits the lymphatic absorption of cholesterol in rats (Hagiwara et al, 1986;Ikeda et al, 1988).…”

Section: Introdutionmentioning

confidence: 97%

Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

Hwang¹,

Jang²,

Lim³

2012

Fisheries and aquatic sciences

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We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative lightscattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%. Key words: Pelvetia siliquosa, Fucosterol, High-speed countercurrent chromatography, Evaporative light-scattering detection IntrodutionPelvetia siliquosa ("Tumbugi" in Korean) belongs to the family Fucaceae. It is a marine alga endemic to the Korean peninsula and grows on the craggy surfaces of the southern seashores (Yoon, 1995). It has been used traditionally similarly to seasoned sea greens for religious services and as a health food (Oh et al., 1990).Results of our previous studies indicate that the ether fraction of P. siliquosa exhibits hepatoprotective and glucoselowering effects, and fucosterol isolated from P. siliquosa has antioxidant and antidiabetic activities (Lee et al., 2002(Lee et al., , 2003(Lee et al., , 2004b. Additionally, it has been reported that fucosterol decreases angiotensin-converting enzyme levels, with a reduction in the glucocorticoid receptor in endothelial cells and inhibits the lymphatic absorption of cholesterol in rats (Hagiwara et al., 1986;Ikeda et al., 1988). Thus, fucosterol is a major component responsible for the various activities of the algae. Fucosterol is usually isolated by extraction with a non-polar solvent and silica-gel column chromatography and is analyzed as the trimethylsilyl derivatives by gas chromatography. We have previously reported a method for the quantitative determination of fucosterol in marine algae to accurately determine their composition and thus generate fingerprints. The structural identification of fucosterol was performed with infrared spectrum (IR), electron impact-mass spectrum (EI-MS), 1 H NMR and 13 C-NMR with tetramethylsilane (TMS) as internal standard. This structure was also confirmed by comparing with the previously reported spectral data. Its structure is shown in Fig. 1 (Lee et al., 2004a).High-speed countercurrent chromatography (HSCCC) is a liquid-liquid partition chromatographic technique that has recently gained increased interest. This technique relies on the use of centrifugal force for the retention of the stationary phase. HSCCC is more advantageous than other liquid-liquid techniques because of the shorter separation time, wider range of possible solvent systems, and quantitative material recovery Hostettmann, 1994, 2006;Ito, 2005 Original ArticleFish Aquat Sci 15(3), [191][192][193][194][195] 2012 Fish Aquat Sci 15(3), [191][192][193][194][195] 2012 http://dx.doi.org/10.5657/FAS.20...

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“…Phlorotannins have been clarified to exhibit anti-diabetic (Lee, Shin, Kim, & Lee, 2004), anti-oxidation (Ahn et al, 2007), anti-cancer (Kong, Kim, Yoon, & Kim, 2009;Yang, Zeng, Dong, Liu, & Li, 2010), and anti-HIV (Ahn et al, 2004) (Table 1) activities.…”

Section: Phlorotanninsmentioning

confidence: 99%

Bioactive potential and possible health effects of edible brown seaweeds

Gupta¹,

Abu‐Ghannam²

2011

Trends in Food Science & Technology

510

292

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Marine macroalgae (seaweeds) are rich in bioactive compounds that could potentially be exploited as functional ingredients for both human and animal health applications. Despite the intensive efforts that are being made to isolate and identify new compounds with potential medicinal, health or pharmaceutical activities, very few compounds with real potency are available. Bioactive compounds that are most extensively researched include sulfated polysaccharides, phlorotannins and diterpenes. These compounds have been reported to possess strong anti-viral, anti-tumor and anti-cancer properties. At the same time, the prebiotic health potential of the polysaccharides from seaweeds is also increasingly being studied either by feeding whole seaweeds or purified polysaccharides to laboratory and farm animals. The present review discusses the pharmaceutical, health and research potential of different bioactive compounds present in brown seaweeds.

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Anti-Diabetic activities of fucosterol fromPelvetia siliquosa

Cited by 124 publications

References 6 publications

Antidiabetic potential of marine algae by inhibiting key metabolic enzymes

Antidiabetic potential of marine algae by inhibiting key metabolic enzymes

Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography

Bioactive potential and possible health effects of edible brown seaweeds

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