Anti-Diabetic and Hepato-Renal Protective Effects of Ziyuglycoside II Methyl Ester in Type 2 Diabetic Mice

Abstract

Type 2 diabetes is a metabolic disorder caused by abnormal carbohydrate metabolism, and closely associated with abnormal lipid metabolism and hepato-renal dysfunction. This study investigated the anti-diabetic and hepato-renal protective properties of ziyuglycoside I (ZG01) derivative on type 2 diabetes. ZG01 was isolated from roots of Sanguisorba officinalis and chemically modified by deglycosylation and esterification to obtained ziyuglycoside II methyl ester (ZG02-ME). Here, we showed that ZG02-ME has stronger anti-diabetic activity than the original compound (ZG01) through decreasing blood glucose, glycated hemoglobin (HbA1c), and insulin levels in a mouse model of type 2 diabetes (db/db mice). We further found that ZG02-ME treatment effectively ameliorated serum insulin, leptin and C-peptide levels, which are key metabolic hormones, in db/db mice. In addition, we showed that elevated basal blood lipid levels were decreased by ZG02-ME treatment in db/db mice. Furthermore, treatment of ZG02-ME significantly decreased serum AST, ALT, BUN, creatinine, and liver lipid peroxidation in db/db mice. These results demonstrated that compared to ZG01, chemically modified ZG02-ME possess improved anti-diabetic properties, and has hepato-renal protective activities in type 2 diabetes.

Simultaneous Determination and Pharmacokinetics Study of Six Triterpenes in Rat Plasma by UHPLC-MS/MS after Oral Administration of Sanguisorba officinalis L. Extract

“…Sanguisorba officinalis L. (S. officinalis) , which is a traditional Chinese medicine (TCM), and a member of Rosaceae family, has the effects of detoxification, analgesic [ 1 ] and hemostatic [ 2 ]. According to the Chinese pharmacopoeia, it plays a major role in the treatment of hematochezia, bleeding hemorrhoids, bloody flux, metrorrhagia and metrostaxis, bleeding wounds, burns and scalds, and swollen carbuncles [ 3 ]. Besides, in vivo and in vitro studies have illustrated that plants from the S. officinalis present a wide range of pharmacological properties, including hemostatic, antioxidant [ 4 ], anti-inflammatory [ 5 ], antiviral [ 6 ], antibacterial [ 7 , 8 ], anti-tumor [ 9 ], neuroprotective and hypoglycemic activities [ 10 ].…”

The Modulative Effects of Microcurrent Electrical Nerve Stimulation on Diabetic Mice

“…6B). The functional biochemical variables, BUN, creatinine, AST, and ALT, associated with kidney and liver functions were significantly higher than normal individuals in diabetes-induced model (3,32). The biochemical functional indicators of kidney, BUN and creatinine, showed also significant amelioration as corresponding to pathological observations in DM+MENS group.…”

Determination of Triterpenoids and Phenolic Acids from Sanguisorba officinalis L. by HPLC-ELSD and Its Application

“…The dried roots of S. officinalis (Rosaceae) and Sanguisorba officinalis var longifolia have been officially listed in the Chinese Pharmacopoeia (2020 edition) [1]. It has the functions of cooling blood, stopping bleeding, detoxifying, and curbing sores [2]. It has been widely used in the treatment of colitis, ulcerative colitis, pyelonephritis, hemostasis, and burns [3].…”