Anti-diabetic study of vitamin B6 on hyperglycaemia induced protein carbonylation, DNA damage and ROS production in alloxan induced diabetic rats

Abdullah, K.M.; Qais, Faizan Abul; Hasan, Hamza; Naseem, Imrana

doi:10.1039/c9tx00089e

Cited by 20 publications

(9 citation statements)

References 38 publications

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“…Muellenbach and coworker [113] demonstrated that the combined treatment of PM and alpha lipoic acid (a powerful antioxidant molecule) resulted in a substantial reduction of the oxidative stress biomarkers in obese Zucker rats. More recently Abdullah and coworkers [114] demonstrated that PM supplementation in alloxan-induced diabetic rats caused a significant decrease in both oxidative stress parameters and ROS production, and consequently reduced DNA damage.…”

Section: Vitamin B6 and Diabetes Complicationsmentioning

confidence: 99%

Vitamin B6 and Diabetes: Relationship and Molecular Mechanisms

Mascolo

Vernı̀

2020

IJMS

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Vitamin B6 is a cofactor for approximately 150 reactions that regulate the metabolism of glucose, lipids, amino acids, DNA, and neurotransmitters. In addition, it plays the role of antioxidant by counteracting the formation of reactive oxygen species (ROS) and advanced glycation end-products (AGEs). Epidemiological and experimental studies indicated an evident inverse association between vitamin B6 levels and diabetes, as well as a clear protective effect of vitamin B6 on diabetic complications. Interestingly, by exploring the mechanisms that govern the relationship between this vitamin and diabetes, vitamin B6 can be considered both a cause and effect of diabetes. This review aims to report the main evidence concerning the role of vitamin B6 in diabetes and to examine the underlying molecular and cellular mechanisms. In addition, the relationship between vitamin B6, genome integrity, and diabetes is examined. The protective role of this vitamin against diabetes and cancer is discussed.

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Section: Vitamin B6 and Diabetes Complicationsmentioning

confidence: 99%

Vitamin B6 and Diabetes: Relationship and Molecular Mechanisms

Mascolo

Vernı̀

2020

IJMS

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“…Endogenous AGEs abundancy can be influenced by the diet, in particular, the amount of reducing sugars in the diet [ 14 , 15 ]. Diet rich in antioxidants leads to decreased AGEs formation both in vitro and in vivo, implicating redox regulation and blood glucose as potential modulatory factors [ 16 , 17 ]. Quercetin (QUE), a plant flavonoid, has antioxidant properties and leads to the inhibition of AGEs formation [ 17 ], and has shown a beneficial effect in diabetes, neurodegenerative diseases and addiction [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster

Vujnović

Jović

Pištan³

et al. 2021

Biomolecules

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Non-enzymatic glycation and covalent modification of proteins leads to Advanced Glycation End products (AGEs). AGEs are biomarkers of aging and neurodegenerative disease, and can be induced by impaired neuronal signaling. The objective of this study was to investigate if manipulation of dopamine (DA) in vitro using the model protein, bovine serum albumin (BSA), and in vivo using the model organism Drosophila melanogaster, influences fluorescent AGEs (fAGEs) formation as an indicator of dopamine-induced oxidation events. DA inhibited fAGEs-BSA synthesis in vitro, suggesting an anti-oxidative effect, which was not observed when flies were fed DA. Feeding flies cocaine and methamphetamine led to increased fAGEs formation. Mutants lacking the dopaminergic transporter or the D1-type showed further elevation of fAGEs accumulation, indicating that the long-term perturbation in DA function leads to higher production of fAGEs. To confirm that DA has oxidative properties in vivo, we fed flies antioxidant quercetin (QUE) together with methamphetamine. QUE significantly decreased methamphetamine-induced fAGEs formation suggesting that the perturbation of DA function in vivo leads to increased oxidation. These findings present arguments for the use of fAGEs as a biomarker of DA-associated neurodegenerative changes and for assessment of antioxidant interventions such as QUE treatment.

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“…Mounting evidence associates vitamin B6 with diabetes, indicating that its deficiency can induce insulin resistance, while its supplementation can restore normal blood glucose levels (Abdullah et al, 2019; Fields et al, 2021; Kim et al, 2018). However, underlying mechanisms are still unknown.…”

Section: Resultsmentioning

confidence: 99%

Vitamin B6 rescues insulin resistance and glucose‐induced DNA damage caused by reduced activity of Drosophila PI3K

Mascolo

Liguori

Merigliano

et al. 2022

Journal Cellular Physiology

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The insulin signaling pathway controls cell growth and metabolism, thus its deregulation is associated with both cancer and diabetes. Phosphatidylinositol 3-kinase (PI3K) contributes to the cascade of phosphorylation events occurring in the insulin pathway by activating the protein kinase B (PKB/AKT), which phosphorylates several substrates, including those involved in glucose uptake and storage. PI3K inactivating mutations are associated with insulin resistance while activating mutations are identified in human cancers. Here we show that RNAiinduced depletion of the Drosophila PI3K catalytic subunit (Dp110) results in diabetic phenotypes such as hyperglycemia, body size reduction, and decreased glycogen content. Interestingly, we found that hyperglycemia produces chromosome aberrations (CABs) triggered by the accumulation of advanced glycation endproducts and reactive oxygen species. Rearing PI3K RNAi flies in a medium supplemented with pyridoxal 5′-phosphate (PLP; the catalytically active form of vitamin B6) rescues DNA damage while, in contrast, treating PI3K RNAi larvae with the PLP inhibitor 4-deoxypyridoxine strongly enhances CAB frequency. Interestingly, PLP supplementation rescues also diabetic phenotypes. Taken together, our results provide a strong link between impaired PI3K activity and genomic instability, a crucial relationship that needs to be monitored not only in diabetes due to impaired insulin signaling but also in cancer therapies based on PI3K inhibitors. In addition, our findings confirm the notion that vitamin B6 is a good natural remedy to counteract insulin resistance and its complications.

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Anti-diabetic study of vitamin B6 on hyperglycaemia induced protein carbonylation, DNA damage and ROS production in alloxan induced diabetic rats

Abstract: Study of nutraceutical and food supplements especially vitamins against diabetes.

Cited by 20 publications

References 38 publications

Vitamin B6 and Diabetes: Relationship and Molecular Mechanisms

Vitamin B6 and Diabetes: Relationship and Molecular Mechanisms

Influence of Dopamine on Fluorescent Advanced Glycation End Products Formation Using Drosophila melanogaster

Vitamin B6 rescues insulin resistance and glucose‐induced DNA damage caused by reduced activity of Drosophila PI3K

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