Anti‐Endometriotic Effects of Pueraria Flower Extract  in Human Endometriotic Cells and Mice

Kim, Jihyun; Woo, Jeong-Hwa; Kim, Hye Mi; Oh, Myung Sook; Jang, Dae Sik; Choi, Jung‐Hye

doi:10.3390/nu9030212

Cited by 27 publications

(26 citation statements)

References 55 publications

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“…Recent studies showed that this pathway plays an important role in the initiation and progression of Ems ( 51 , 52 ). Liu et al ( 51 ) previously found that ERK signaling contributed to the enhanced invasiveness of ectopic ESCs, whilst Kim et al ( 53 ) reported that ERK1/2 signaling was involved in endometriotic cell migration induced by peritoneal fluid from individuals with EMS. These previous studies suggest that repressing the activation of this signaling pathway may prove to be a viable option for treating this condition.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

et al. 2020

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The enhanced migratory ability of endometrial stromal cells (ESCs) is a key factor in the formation of functional endometrium-like tissues outside the uterine cavity during endometriosis (EMS). Although accumulating evidence has suggested the importance of microRNAs (miRNAs) in the pathogenesis of EMS, the role of particular miRNAs in the invasiveness of ESCs remain poorly understood. In the present study, the function of miRNAs in the invasiveness of ESCs, along with the associated underlying mechanism involved, were investigated. Initially, the expression patterns of miRNAs in the ectopic and eutopic endometrium isolated from patients with EMS were analyzed using microarray. MicroRNA-202-5p (miR-202) was selected for further study due to its previously reported suppressive effects on the invasion in various types of cancers. The expression of miR-202 and K-Ras in eutopic and ectopic endometrioma tissues were detected using reverse transcription-quantitative PCR, immunohistochemistry and western blotting. The migration and invasion ability of ESCs was determined using wound healing and Transwell invasion assays, respectively. Compared with that from healthy individuals, miR-202 expression was demonstrated to be lower in the eutopic endometrium from patients with EMS, which was even lower in ectopic endometrium. Functional experiments in primary ESCs revealed that enhanced miR-202 expression suppressed the cell invasion and migration abilities, which was also accompanied with increased E-cadherin and reduced N-cadherin expression in ESCs, suggesting its potentially suppressive role in epithelial-mesenchymal transition. K-Ras is a well-known regulator of the ERK signaling pathway that was shown to be directly targeted and negatively regulated by miR-202. In addition, K-Ras expression was found to be upregulated in the ectopic endometrium, where it correlated negatively with that of miR-202. Knocking down K-Ras expression mimicked the anti-invasive effects of miR-202 overexpression on ESCs, whilst K-Ras overexpression attenuated the inhibitory role of miR-202 overexpression in ESC invasion. The K-Ras/Raf1/MEK/ERK signaling pathway was also blocked by miR-202 overexpression. These findings suggested that miR-202 inhibited ESC migration and invasion by inhibiting the K-Ras/Raf1/MEK/ERK signaling pathway, rendering miR-202 a candidate for being a therapeutic target for EMS.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

et al. 2020

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“…These data provide additional support for the safety of KM use [ 27 , 28 ]. Kim et al [ 35 ] presented an in vitro study highlighting a potential role of kudzu flower extract as an anti-endometriotic agent with inhibition against endometriotic cell adhesion and migration. In a mouse model of menopause, Sternlicht et al [ 36 ] showed that kudzu flower extract exerted a noticeable down-regulation of matrix metalloproteinase, which regulates migration, invasion, and proliferation of various cells.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and Safety of Kudzu Flower–Mandarin Peel on Hot Flashes and Bone Markers in Women during the Menopausal Transition: A Randomized Controlled Trial

et al. 2020

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This randomized controlled study aimed to assess the efficacy and safety of an extract mixture of kudzu flower and mandarin peel (KM) on hot flashes (HFs) and markers of bone turnover in women during the menopausal transition. Healthy women aged 45–60 years with the menopausal HFs were randomly assigned in a 1:1 ratio to either KM (1150 mg/day) or placebo arms for 12 weeks (n = 84). The intent-to-treat analysis found that compared with the placebo, the KM significantly attenuated HF scores (p = 0.041) and HF severities (p < 0.001), with a mean difference from baseline to week 12. The KM also improved bone turnover markers, showing a significant reduction in bone resorption CTx (p = 0.027) and a tendency of increasing bone formation OC relative to the placebo. No serious adverse events and hormonal changes were observed in both groups. These findings suggest that KM consumption may improve the quality of life in ways that are important to symptomatic menopausal women.

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“…In previous study, MMP-2 and MMP-9 increased, meanwhile TIMP-1 decreased in EMS ( Huang et al, 2015 ; Yi et al, 2015 ; Jana et al, 2016 ; Szymanowski et al, 2016 ). The therapy, through suppressing MMP-2 and MMP-9, promoting TIMP-1, have been reported as the effective methods on EMS ( Huang et al, 2015 ; Li et al, 2016 ; Kim et al, 2017 ). Consistently, in our experiment, JFS inhibited the growth of ectopic endometrium and recurred the pathological changes.…”

Section: Discussionmentioning

confidence: 99%

Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology

et al. 2018

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Jiawei Foshou San (JFS) is the new formula originated from classic Foshou San formula, composed with ligustrazine, ferulic acid, and tetrahydropalmatine. Previously JFS inhibited the growth of endometriosis (EMS) with unclear mechanism, especially in metastasis, invasion, and epithelial–mesenchymal transition. In this study, network pharmacology was performed to explore potential mechanism of JFS on EMS. Through compound–compound target and compound target–EMS target networks, key targets were analyzed for pathway enrichment. MMP–TIMP were uncovered as one cluster of the core targets. Furthermore, autologous transplantation of EMS rat’s model were used to evaluate in vivo effect of JFS on invasion, metastasis and epithelial–mesenchymal transition. JFS significantly suppressed the growth, and reduced the volume of ectopic endometrium, with modification of pathologic structure. In-depth study, invasion and metastasis were restrained after treating with JFS through decreasing MMP-2 and MMP-9, increasing TIMP-1. Meanwhile, JFS promoted E-cadherin, and attenuated N-cadherin, Vimentin, Snail, Slug, ZEB1, ZEB2, Twist. In brief, anti-EMS effect of JFS might be related to the regulation of epithelial–mesenchymal transformation, thereby inhibition of invasion and metastasis. These findings reveal the potential mechanism of JFS on EMS and the benefit for further evaluation.

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Anti‐Endometriotic Effects of Pueraria Flower Extract in Human Endometriotic Cells and Mice

Cited by 27 publications

References 55 publications

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

MicroRNA‑202 inhibits endometrial stromal cell migration and invasion by suppressing the K‑Ras/Raf1/MEK/ERK signaling pathway

Efficacy and Safety of Kudzu Flower–Mandarin Peel on Hot Flashes and Bone Markers in Women during the Menopausal Transition: A Randomized Controlled Trial

Anti-endometriosis Mechanism of Jiawei Foshou San Based on Network Pharmacology

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