Anti‐endothelial cell antibodies to the endothelial hybridoma cell line (EAhy926) in systemic lupus erythematosus patients with antiphospholipid antibodies

Matsuda, Juzo; Gotoh, Moritaka; Gohchi, Kengo; Kawasugi, Kazuo; Tsukamoto, Mitsuo; Saitoh, Noriko

doi:10.1046/j.1365-2141.1997.d01-2147.x

Cited by 23 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with the phenotypic variation in endothelial cells of dierent origins, and con®rm the frequent presence of autoantibodies reactive with vascular endothelium in both SLE and primary antiphospholipid syndrome. Although it is possible for anti-cardiolipin antibody and AECA to be partially cross-reacting antibodies [29], AECA is an endothelial cell-speci®c antibody and is dierent to anti-cardiolipin antibody [9,30,31]. In our preliminary immunoblotting study with membrane preparations of HMEC-1, we tested sera from seven patients with high titers of AECA in ELISA and found that AECA binds dierent molecular sizes (19,30,34,40, and 45 kDa).…”

Section: Discussionmentioning

confidence: 99%

Clinical associations of anti-endothelial cell antibodies in patients with systemic lupus erythematosus

Song

Park²,

Lee

et al. 2000

Rheumatology International

View full text Add to dashboard Cite

The aim of this study was to define the clinical associations of anti-endothelial cell antibody (AECA) in systemic lupus erythematosus (SLE) patients by measuring serum AECA titers to correlate with the disease activity and clinical manifestations. Forty-one SLE patients and 27 controls were studied. Serum samples were collected at the time of patient presentation with disease exacerbation and 4 weeks after the start of treatment. The disease activity was evaluated by the SLE Disease Activity Index (SLEDAI). AECA was detected by enzyme-linked immunosorbent assay (ELISA) methods with the surface antigen of the immortalized human microvascular endothelial cell line (HMEC-1). The mean immunoglobulin (Ig)G-AECA and IgM-AECA optical densities (ODs) were significantly higher in patients with SLE compared with controls [mean +/- standard deviation (SD), 0.32 +/- 0.15 vs 0.18 +/- 0.16 and 0.29 +/- 0.14 vs 0.21 +/- 0.09, respectively]. There was a positive correlation between IgG-AECA and the SLEDAI scores. The positivity rate of AECA in the groups with digital vasculitis, neuropsychiatric lupus, and anti-cardiolipin antibody was significant. In conclusion, AECA may be involved in the pathogenesis of SLE and was correlated with the disease activity. It was also associated with clinical manifestations such as digital vasculitis, neuropsychiatric lupus, and anti-cardiolipin antibody positivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Clinical associations of anti-endothelial cell antibodies in patients with systemic lupus erythematosus

Song

Park²,

Lee

et al. 2000

Rheumatology International

View full text Add to dashboard Cite

show abstract

“…This phenomenon was observed in SSc and may be mediated through CD95 (Fas) [53], although this point is controversial [54]. Another mechanism for apoptosis induction by AECA could implicate antiphospholipid antibodies, which could crossreact with AECA [55]. Antiphospholipid antibodies could interact with EC through the recognition of beta2-GPI adherent to EC surface [4].…”

Section: Induction Of Coagulationmentioning

confidence: 99%

Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Guilpain

Mouthon

2008

Clinic Rev Allerg Immunol

View full text Add to dashboard Cite

Antiendothelial cell antibodies (AECA) have been detected in healthy individuals, as well as in autoimmune and systemic inflammatory diseases, including systemic vasculitides. AECA have been reported in large vessel vasculitides such as giant cell arteritis and Takayasu arteritis; medium-sized vessel vasculitides, such as polyarteritis nodosa related to hepatitis B virus infection and Kawasaki disease; and small-sized vessel vasculitides, such as Wegener's granulomatosis, microscopic polyangiitis, and Henoch-Schonlein purpura. In Takayasu arteritis and antineutrophil cytoplasm antibody-positive vasculitides, AECA have been reported to correlate with disease activity. A cell-based enzyme-linked immunosorbent assay (ELISA) using cultured human umbilical vein endothelial cells (HUVEC) represent one of the reference techniques for AECA detection, although flow cytometry and immunobloting have also been proposed. AECA might contribute to the pathogenesis of systemic vasculitides and vasculitis-associated diseases through (1) activation of endothelial cells (EC), (2) direct cytotoxic effect due to complement-dependent cytotoxicity or indirect cytotoxic effect secondary to antibody-dependent cytotoxicity, (3) induction of coagulation, (4) induction of apoptosis through the binding of phospholipids or heat-shock protein 60, and (5) induction of EC activation. None of the identified target antigens of AECA is specific for EC, and EC-specific target antigens of AECA remain to be identified in systemic vasculitides.

show abstract

“…Synovial cells of patients with rheumatoid arthritis (RA), collected from synovium which was resected during surgery, were used for culture. 16 …”

Section: Cell Culturementioning

confidence: 97%

Endothelial cell-binding antibodies in patients with systemic lupus erythematosus

Bando

Tamura

Kobayashi

et al. 2003

Modern Rheumatology

View full text Add to dashboard Cite

The implications of endothelial cell-binding IgG antibodies (EC IgG) in systemic lupus erythematosus (SLE) was evaluated by determining level of EC IgG in sera from 112 SLE patients. The serum EC IgG level was determined by the cyto-ELISA method using human umbilical vein endothelial cells (HUVEC), human microvascular endothelial cells (HMVEC), and aortic endothelial cells (HAEC) as antigens. The levels of EC IgG were significantly higher among patients with SLE than among healthy control subjects (P Ͻ 0.001), and 68% (76/112) of SLE patients were shown to be EC IgG-positive. In patients with active lupus nephritis, the level of EC IgG was statistically and significantly elevated compared with those without lupus nephritis (P Ͻ 0.05). Negative correlations between EC IgG level and levels of CH50, C3, and lymphocyte count were revealed (P Ͻ 0.05, P Ͻ 0.005, and P Ͻ 0.05, respectively). When clinical course was evaluated, the levels of EC IgG correlated with disease activity. Definitive correlations in antibody levels between HUVEC and HMVEC, and between HUVEC and HAEC were revealed (both P Ͻ 0.0001). The results of this study revealed that the EC IgG in patients with SLE was involved in the onset of clinical manifestation, especially in patients with active lupus nephritis.

show abstract

Anti‐endothelial cell antibodies to the endothelial hybridoma cell line (EAhy926) in systemic lupus erythematosus patients with antiphospholipid antibodies

Cited by 23 publications

References 21 publications

Clinical associations of anti-endothelial cell antibodies in patients with systemic lupus erythematosus

Clinical associations of anti-endothelial cell antibodies in patients with systemic lupus erythematosus

Antiendothelial Cells Autoantibodies in Vasculitis-Associated Systemic Diseases

Endothelial cell-binding antibodies in patients with systemic lupus erythematosus

Contact Info

Product

Resources

About