Anti-Fatigue Activity of a Mixture of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. Fruit Extract

hyun, Oh joo; Han, Yoonyoung; Kim, Jimin; Sung, Park Chan; Oh, Dool-Ri; Jeong, Yun Hyo; Lee, Gyuok; Yong, Kim Jae; Choi, Chulyung; Lee, Yongwook

doi:10.3746/pnf.2020.25.4.380

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…These studies provided novel insights for understanding VBT and identified prospects for exploration of VBT products. However, most of these studies focused on chemical compounds 4 , 7 , 11 , 16 , 17 , and very few studies have concentrated on VBT’s genetic and biochemically active molecules, which may promote its application in improving the breeding of crops.…”

Section: Introductionmentioning

confidence: 99%

Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR

Gui

Zhang

et al. 2022

Sci Rep

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Vaccinium bracteatum Thunb. (VBT) is widely distributed in the mountainous areas in eastern and southern China. VBT leaves have great medical value and can be used to stain rice to produce “Wumifan”. Its fruits also contain rich nutrients. However, there has been limited attention to exploring the molecular content of VBT. Previously, we performed RNA-seq on three typical VBT fruits that were at various stages of ripening, although a reliable reference gene was lost in validation.In this study, we selected ten candidate reference genes based on previous studies and transcriptomics analyses. Subsequently, these genes were evaluated using a combination of methods, including geNorm, NormFinder, and Bestkeeper, with a comprehensive ranking assessment. As a result, we found that the actin2, NADH, and ADK genes have high reliability for analysing the expression levels of genes involved in fruit development. Furthermore, the transcript levels of 15 DEGs from transcriptomic analysis were assessed using NADH as a reference gene, and RT-qPCR data were highly consistent with the transcriptomic data. These results provide reliable reference genes for further studying gene expression, which will be beneficial for comprehensively exploring VBT.

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Section: Introductionmentioning

confidence: 99%

Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR

Gui

Zhang

et al. 2022

Sci Rep

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“…According to the free radical theory, the accumulation of oxidative damage is a causal factor for muscle fatigue ( 30 ). Thus, H 2 O 2 exposure was widely used for inducing C2C12 cell injuries to mimic oxidative damage in muscle fatigue ( 31 , 32 ). Commonly, cell viability is a comprehensive index used to evaluate proliferation and cell death, indicating the potential of MCP to resist fatigue.…”

Section: Resultsmentioning

confidence: 99%

Deciphering the potential role of Maca compounds prescription influencing gut microbiota in the management of exercise-induced fatigue by integrative genomic analysis

et al. 2022

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A growing number of nutraceuticals and cosmeceuticals have been utilized for millennia as anti-fatigue supplements in folk medicine. However, the anti-fatigue mechanism underlying is still far from being clearly explained. The aim of the study is to explore the underlying mechanism of the Maca compound preparation (MCP), a prescription for management of exercise-induced fatigue. In this study, mice weight-loaded swimming test was used to evaluate the anti-fatigue effect of MCP. MCP significantly improved the forelimb grip strength and Rota-rod test in behavioral tests via regulating energy metabolism. 16S rDNA sequencing results showed MCP can regulate the intestinal flora at the genus level by increasing several beneficial bacteria (i.e., Lactobacillus, Akkermansia and etc.), and decreasing the harmful bacteria (i.e., Candidatus_Planktophila and Candidatus_Arthromitus), where notable high relevance was observed between the fatigue-related biomarkers and fecal microbiota. The results of microbial function analysis suggested that MCP might improve exercise-induced fatigue by enhancing energy metabolism, carbohydrate and lipid metabolism and metabolism of terpenoids and polyketides and breakdown of amino acid metabolism. In addition, and H2O2-induced oxidative stress model on C2C12 cells was employed to further validate the regulation of MCP on energy metabolisms. MCP pre-treatment significantly reduced intracellular ROS accumulation, and increased glycogen content, ATP generation capacity and mitochondrial membrane potential of skeletal muscle cells, as well as conferred anti-cell necrosis ability. In conclusion, MCP plays a key role in regulating fatigue occurrence in exercising and gut microbiota balance, which may be of particular importance in the case of manual workers or sub-healthy populations.

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“…27 COX III is the main constituent subunit of COX, which is responsible for the regulation of COX assembly and antioxidative enzyme activity. 24 In addition, CPT1, UCP3, and PPAR-δ play an important role in regulating fatty acid β-oxidation during mitochondrial transport, 28 and UCP3 can control the ROS production. 29 A current study showed that Maca ( Lepidium meyenii Walp .)…”

Section: Discussionmentioning

confidence: 99%

Antifatigue effect of naringin on improving antioxidant capacity and mitochondrial function and preventing muscle damage

Chen

Xue

Jia

et al. 2022

Exp Biol Med (Maywood)

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The aim of this study was to explore effects of naringin (Nar) on antifatigue ability; the weight-loaded and non-loading swimming tests were performed. Compared with the control group, dietary supplementation of Nar significantly prolonged the weight-loaded swimming time to exhaustion of mice ( P < 0.01). Nar significantly reduced the serum lactic acid (LD) level ( P < 0.05) and lactate dehydrogenase (LDH) activity ( P < 0.001), while increased the serum non-esterified free fatty acids (NEFA) level ( P < 0.001). In addition, Nar significantly increased the liver glycogen and muscle glycogen contents ( P < 0.05) and the phosphoenolpyruvate carboxykinase ( PEPCK) ( P < 0.01) and glucokinase ( GCK) mRNA levels ( P < 0.001) in liver and gastrocnemius (GAS) muscle. Furthermore, Nar significantly improved the antioxidant capacity, mitochondrial function, and muscle mitochondrial fatty acid β-oxidation ( P < 0.05), and decreased inflammation and muscle damage–related gene expression ( P < 0.05). These findings suggested that Nar can improve antifatigue effect by enhancing antioxidant capacity and mitochondrial function and preventing muscle damage.

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Anti-Fatigue Activity of a Mixture of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. Fruit Extract

Cited by 6 publications

References 34 publications

Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR

Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR

Deciphering the potential role of Maca compounds prescription influencing gut microbiota in the management of exercise-induced fatigue by integrative genomic analysis

Antifatigue effect of naringin on improving antioxidant capacity and mitochondrial function and preventing muscle damage

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