Anti-Fatigue Effect of a Dietary Supplement from the Fermented By-Products of Taiwan Tilapia Aquatic Waste and Monostroma nitidum Oligosaccharide Complex

Abstract

The Taiwan Tilapia is an important aquaculture product in Taiwan. The aquatic by-products generated during Tilapia processing, such as fish bones and skin, are rich in minerals and protein. We aimed to explore the effect of a dietary supplement, comprising a mixture of fermented Tilapia by-products and Monostroma nitidum oligosaccharides as the raw materials, combined with physical training on exercise performance and fatigue. We used a mouse model that displays a phenotype of accelerated aging. Male senescence-accelerated mouse prone-8 (SAMP8) mice were divided into two control groups—with or without physical training—and supplemented with different doses (0.5 times: 412 mg/kg body weight (BW)/day; 1 time: 824 mg/kg BW/day; 2 times: 1648 mg/kg BW/day) of fermented Tilapia by-products and Monostroma nitidum oligosaccharide-containing mixture and combined with exercise training groups. Exercise performance was determined by testing forelimb grip strength and with a weight-bearing exhaustive swimming test. Animals were sacrificed to collect physical fatigue-related biomarkers. Mice dosed at 824 or 1648 mg/kg BW/day showed improvement in their exercise performance (p < 0.05). In terms of biochemical fatigue indicators, supplementation of 824 or 1648 mg/kg BW/day doses of test substances could effectively reduce blood urea nitrogen concentration and lactate concentration and increase the lactate ratio (p < 0.05) and liver glycogen content post-exercise (p < 0.05). Based on the above results, the combination of physical training and consumption of a dietary supplementation mixture of fermented Tilapia by-products and Monostroma nitidum oligosaccharides could improve the exercise performance of mice and help achieve an anti-fatigue effect.

Comparative Study of the Components and Anti-Fatigue Effect of Schisandra chinensis Polysaccharides from China and Russia

“…Polysaccharides and their complexes are not only used as energy resources and building materials in organisms, but also exist in all cell membrane structures and participate in the activity of various cells. 14 Comparison of the results of forelimb grip strength, weight-bearing swimming and the rota-rod test may objectively reflect the differences in the endurance of mice among the experimental groups. 15 The results of the three tests showed that the anti-fatigue effect of Russian S. chinensis was better than that of the Chinese, which may verify the conclusion based on the polysaccharide composition detection, as described above.…”

Trilobatin, a Naturally Occurring Food Additive, Ameliorates Exhaustive Exercise-Induced Fatigue in Mice: Involvement of Nrf2/ARE/Ferroptosis Signaling Pathway

“…LG and MG, which act as a substrate source for glycolysis and energy production, are also key indicators of EEIF (Chen et al, 2021).…”

The Effect of Hot Water Extract of Tilapia on Exercise Capacity in Mice

“…Our results showed that oral administration of HWET significantly increased glycogen content in the liver, but only slightly enhanced that of muscle tissue (Figure 4). Chen et al [38] also indicated that a mixture of fermented tilapia by-products and Monostroma nitidum oligosaccharides significantly increased liver glycogen content and decreased BUN level post-exercise. It has been proposed that the re-synthesis of liver glycogen is the first priority when glucose is available after exercise, followed by the synthesis of muscle glycogen [39].…”