Anti-fibrotic effect of Holothuria arenicola extract against bile duct ligation in rats

Abstract

BackgroundHolothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats.MethodsFifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight).ResultsThe HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities.ConclusionThe present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.

Testicular immunohistochemical and Ultrastructural changes associated with chronic cholestasis in rats: Effect of ursodeoxycholic acid

“…Alteration of tight junction structure was also observed in cells exposed to toxic bile acids [30,31]. On the other hand, cholestasis is correlated with systemic oxidative stress [32,33], which is thought to play a key role in the associated cellular injury [34,35].…”

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxicity and cholestasis in bile duct ligation rats: involvement of SIRT1‐mediated deacetylation of FXR and PGC‐1α

“…Artemisia capillaris (Yin‐Chen) is another commonly used traditional herb rich in CA . CA has been shown to alleviate liver injury induced by bile duct ligation (BDL) in rats and to decrease liver fibrosis through decreased expression of collagen I and III, transforming growth factors, and vascular epithelial growth factor . Nevertheless, the mechanisms involved in the choleretic effect of CA are not fully understood.…”

Attenuation of Carbon Tetrachloride and Ethanol-Induced Hepatic Fibrosis in Rats by Calligonum Comosum Shoot Extract

“…Thus, C. comosum extract improved the decline in liver synthetic function caused by CCl 4 . Stimulation of protein synthesis has been regarded as a contributory hepatoprotective mechanism which enhances the regeneration process and the production of liver cells [54].…”