Anti-fouling materials decorated immunoprobe and electrochemical sensing interface to improve immunoassay

Meng, Xuanze; Xu, Yang; Ma, Bochen; Ma, Zhanfang; Han, Hongliang

doi:10.1016/j.cej.2022.137954

Cited by 15 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Hu et al reported a competitive electrochemical immunosensor for maduramicin detection in which an antibody and HRP were immobilized on hemin@MOFs/AuPt composites via the formation of robust Pt-N and Au-N bonds [32]. Meng et al used HRP and antifouling material-decorated ZIF-90 as the immunoprobe to achieve the electrochemical detection of carbohydrate antigen 19-9 [33]. Zr-MOF PCN-224 has been used to simultaneously load GOx and platinum nanoparticles for the colorimetric detection of deoxynivalenol [34].…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

Sun,

Yi,

Liu

et al. 2024

Molecules

View full text Add to dashboard Cite

The sensitivity of immunoassays is generally limited by the low signal reporter/recognition element ratio. Nanomaterials serving as the carriers can enhance the loading number of signal reporters, thus improving the detection sensitivity. However, the general immobilization strategies, including direct physical adsorption and covalent coupling, may cause the random orientation and conformational change in proteins, partially or completely suppressing the enzymatic activity and the molecular recognition ability. In this work, we proposed a strategy to load recognition elements of antibodies and enzyme labels using boronic acid-modified metal-organic frameworks (MOFs) as the nanocarriers for signal amplification. The conjugation strategy was proposed based on the boronate ester interactions between the carbohydrate moieties in antibodies and enzymes and the boronic acid moieties on MOFs. Both enzymes and MOFs could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2, therefore achieving dual signal amplification. To indicate the feasibility and sensitivity of the strategy, colorimetric immunoassays of prostate specific antigen (PSA) were performed with boronic acid-modified Cu-MOFs as peroxidase mimics to catalyze TMB oxidation and nanocarriers to load antibody and enzyme (horseradish peroxidase, HRP). According to the change in the absorbance intensity of the oxidized TMB (oxTMB), PSA at the concentration range of 1~250 pg/mL could be readily determined. In addition, this work presented a site-specific and oriented conjugation strategy for the modification of nanolabels with recognition elements and signal reporters, which should be valuable for the design of novel biosensors with high sensitivity and selectivity.

show abstract

Section: Introductionmentioning

confidence: 99%

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

Sun,

Yi,

Liu

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…The surface wetting property (hydrophilicity/hydrophobicity) of a solid interface is crucial in interface chemistry, dominating the application in the areas of physical separation, − energy storage devices, pharmaceutical developments, , self-cleaning, immunosensors, and electrical insulation. , For centuries, various methods were used to tailor the surface wetting properties, − and corresponding theories had been established. − However, the underlying truth remains a mystery and is still attracting renowned scientists.…”

Section: Introductionmentioning

confidence: 99%

Surprising Hydrophobic Polymer Surface with a High Content of Hydrophilic Polar Groups

Niu

Shang

et al. 2022

Langmuir

View full text Add to dashboard Cite

The wetting property of a solid surface has been a hotspot for centuries, and many studies suggest that the hydrophobicity is highly related to the polar components. However, the underlying mechanism of polar moieties on the hydrophobicity remains unclear. Here, we tailor the surface polar moieties of epoxy resin (EP) by ozone modification and assess their wetting properties. Our results show that, for the modified EP with more (60.54%) polar moieties, the polar effect on hydrophobicity cannot be empirically observed. To reveal the underlying mechanism, the absorption parameters, including equilibrium distance, adsorption radius, and effective adsorption sites for water on EP before and after ozone treatment, are calculated on the basis of molecular simulations. After ozone modification, the equilibrium distance (from 1.95 to 1.70 Å), adsorption radius (from 3.80 to 4.50 Å), and effective adsorption sites (from 1 to 2) change slightly and the EP surface remains hydrophobic, although the polar groups significantly increase. Therefore, it is concluded that the wetting properties of solid surfaces are dominated by the equilibrium distance, adsorption radius, and effective adsorption sites for water on solids, and the nonlinear relationship between polar groups and hydrophilicity is clarified.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Although effective, the major constituent of antifouling surfaces comprising polyethylene glycol and oligoethylene glycols are prone to poor water solubility and oxidative damage . Moreover, the presence of hydrophobic layers on the electrode surfaces often limits the density and alignment of the recognition elements due to agglomeration and irregular distribution of the recognition elements on the electrode surface, demanding a sensitive and reproducible electrochemical response …”

Section: Introductionmentioning

confidence: 99%

“…27 Moreover, the presence of hydrophobic layers on the electrode surfaces often limits the density and alignment of the recognition elements due to agglomeration and irregular distribution of the recognition elements on the electrode surface, demanding a sensitive and reproducible electrochemical response. 28 Despite the growing emphasis on attaining clinically relevant electrochemical responses, the existing breast cancer diagnostics still suffer from significant bottlenecks restricting their commercial utilization. Primarily, interfacial probe density remains the major factor affecting electrochemical performance and mass reproducibility, along with minimal sample processing and consumption requirements.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphology-Tuned Electrochemical Immunosensing of a Breast Cancer Biomarker Using Hierarchical Palladium Nanostructured Interfaces

Joshi

Dhruv³

et al. 2022

ACS Omega

View full text Add to dashboard Cite

Metallic nanostructures are considered attractive candidates for designing novel biosensors due to their enormously significant surface area, accelerated kinetics, and improved affinity. Controllable morphological tuning of metallic nanostructures on sensing interfaces is crucial for attaining clinically relevant sensitivity and exquisite selectivity in a complex biological environment. Therefore, a facile, convenient, and robust one-step electroreduction method was employed to develop different morphological variants of palladium (Pd) nanostructures supported onto oxidized carbon nanotubes to facilitate label-free electrochemical immunosensing of HER2. The morphological and structural attributes of the synthesized Pd nanostructures were thoroughly investigated using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and atomic force microscopy techniques. In-depth electrochemical investigations revealed an intimate correlation between the nanostructured sensor and electrochemical response, suggesting the suitability of hierarchical palladium nanostructures supported onto carbon nanotubes [Pd(−0.1 V)/CNT] for sensitive detection of HER2. The high surface area of hierarchical Pd nanostructures enabled an ultrasensitive electrochemical response toward HER2 (detection limit: 1 ng/mL) with a wide detection range of 10 to 100 ng/mL. The ease of surface modification, sensitivity, and reliable electrochemical response in human plasma samples suggested the enormous potential of Pd nanostructuring for chip-level point-of-care screening of HER2-positive breast cancer patients.

show abstract

Anti-fouling materials decorated immunoprobe and electrochemical sensing interface to improve immunoassay

Cited by 15 publications

References 34 publications

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

Colorimetric Immunoassays with Boronic Acid-Decorated, Peroxidase-like Metal-Organic Frameworks as the Carriers of Antibodies and Enzymes

Surprising Hydrophobic Polymer Surface with a High Content of Hydrophilic Polar Groups

Morphology-Tuned Electrochemical Immunosensing of a Breast Cancer Biomarker Using Hierarchical Palladium Nanostructured Interfaces

Contact Info

Product

Resources

About