Anti-H-2 alloantibodies elicited by syngeneic immunizations with a chemically induced fibrosarcoma

Detection of Cell-Surface Antigens in Murine Tumors by a Protein A Immunofluorescence Test

“…The presence of 'foreign' allospecificities in MC sarcomas has been previously docu mented by Meschini et al [6] and has been interpreted as though they represent the tu mor-specific antigens [7] or that they are as sociated antigens different from those which are tumor specific [23]. It should be noted that anti-MC25 sera is capable of blocking the reactivity of anti-H-2D.13 and anti-H-2D.16 sera on lymphoma cells, both antigens detected on the surface of the MC25 sarcoma cells as 'foreign' [Merino, F.; unpublished observations].…”

“…By these tests, antigeneic individuality or cross-reactivity of the tumor-specific anti gens among different chemically induced tu mors has been reported [2][3][4], H-2 allospecificities of these tumors have been studied by either complement-de pendent cytotoxicity techniques in cultured cells or by the absorption of specific alloantisera with these tumors. H-2 alloantigen ex pression on neoplastic cells seems to play a role in tumor immunity mechanisms since new alloantigens can be detected on the surface of these cells and their possible role as tumorspecific antigens has been postulated [5][6][7].…”

Real and apparent H-2-specific antibodies induced by syngeneic immunization

“…In other experiments H-2-specific antibodies were induced by immunization with syngeneic cells. Various approaches were applied: injection of syngeneic cells (Iv~nyi et al 1980, Capkowi et al 1983) or syngeneic concanavalin A (Con A)-induced blasts (Mieran and Cramer 1984), syngeneic tumor cells (Meschini and Parmiani 1978, Schmidt et al 1987, Opolski et al 1988, virus (Sendai)-coated cells (Opolski et al 1986, or syngeneic cells transfected with a cloned retroviral DNA (Pla et al 1988). The specificities of these anti-H-2 antibodies were variable and unpredictable.…”

Biochemical characterization of alien H-2 antigens expressed on a methylcholanthrene-induced tumor.

“…Recently, a number of observations have suggested that certain tumor cells and virally infected cells may express alien antigens identified with the major histocompatibility locus of an unrelated haplotype (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). These observations have involved the ability of tumor cells to absorb cytotoxic antibody against normal cells of an unrelated haplotype (4), the ability of alloantisera directed against H-2 antigens of an inappropriatehaplotype to lyse cells (7,10), or the unexpected growth of a tumor in immunized hybrid (1,2) or recombinant animals (6).…”

“…Appropriate absorption experiments ruled out the possibility that the ability of these cells to absorb anti-H-2k antisera was due to viral antigens or previously undetected public specificities (4). Furthermore, syngeneic mice immunized against the C-1 tumor produce antisera having properties identical to anti-H-2Kk antisera produced in congenic mice (5). The observation that certain (H-2d X H-2k)Fj mice do not develop immunity to this tumor while H-2d mice do (2) suggests that the alien H-2 antigens expressed on this tumor may play a role in the host antitumor immune response.…”