Anti-HCV Positive Hemodialysis Patients: Clinical, Biochemical, Histologic and Virologic Study and a Proposed Management Scheme

Altraif, Ibrahim; Huraib, Sameer; Abdullah, Abdulqadir; Nondo, Hassan; Said, Riyadh; Duarte, R.; Quadri, K; Alghamdi, Gormallah; Lombardi, S; Wolfe, Larry A.

doi:10.5144/0256-4947.2000.307

Cited by 1 publication

(1 citation statement)

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“…Hepatitis C virus (HCV) infection has reached an epidemic proportion globally and is associated with many extra hepatic manifestations. The prevalence of anti-HCV positivity among dialysis patients varies in different countries (5%-85% worldwide), but may exceed 95% in some hemodialysis units (Al Traif et al, 2000). Hepatitis is considered as one of the important causes of death in chronic renal failure patients undergoing hemodialysis or renal transplantation.…”

Section: Resultsmentioning

confidence: 99%

Comparison of third generation ELISA and conventional nested RT-PCR for detection of HCV among hemodialysis patients

Prakash¹,

Sankhwar²,

Jain³

et al. 2014

J. App. Pharm. Sci.

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Hepatitis C virus is one of the main cause of chronic hepatitis in developing countries. The current study was done to evaluate the efficacy of the third generation ELISA compared to nested RT-PCR for establishing the diagnosis of hepatitis C virus (HCV) in patients on hemodialysis. This descriptive, cross-sectional study was carried out on 237 Hemodialysis patients in Lucknow, Uttar Pradesh. The retrospective demographic data of the subjects was collected and the patient's serum samples were analyzed by ELISA & RT-PCR for HCV. In the present study, of total 21 HCV positive either by ELISA or PCR 12 (57.14%) were positive for both RT-nested PCR and ELISA. Total four (19.05%) patients were positive for HCV by RT-nested PCR and negative by ELISA while five (23.81%) patients were negative for RT-nested PCR and positive for ELISA. PCR method is accredited as a specific and reliable method suitable for screening of HCV and is recommended for establishing exact and final diagnosis of these patients. However third generation ELISA assays have many advantages in the diagnostic setting including ease of automation, ease of use, relative cost-effectiveness, and low variability.

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Section: Resultsmentioning

confidence: 99%