Anti-Helicobacter pylori activity in vitro of chamomile flowers, coneflower herbs, peppermint leaves and thyme herbs – a preliminary report

Abstract

Recently, several studies have been undertaken so as to develop more effective therapeutic approaches towards eradicating Helicobacter pylori. Among these is phytotherapy. The aim of this study was to investigate the activity in vitro of the plant extracts obtained from common herbs cultivated in the Lubelszczyzna region against the reference strain H. pylori ATCC 43504. Among these are thyme herbs, chamomile flowers, peppermint leaves and coneflower herbs. Herein, it was found that the MIC values of the assayed extracts were as follows: the extracts from coneflower herbs showed anti-H. pylori activity with MIC = 31.3-125 μg/ml; the extracts from chamomile flowers demonstrated MIC = 31.3-62.5 μg/ ml; the extracts from peppermint leaves had MIC = 15.6-250 μg/ml; and the extracts from thyme herbs revealed MIC = 15.6-62.5 μg/ml, depending on the solvent used. The most active were the extracts obtained with ethyl acetate or ethanol alcohol absolute 99.8%. These showing MIC within the range of 15.6-62.5 μg/ml, while the lowest activity was observed in case of the extract obtained with 70% aqueous ethanol. This last showing MIC within the range of 62.5-250 μg/ml. The MIC values of essential oil components were 15.6 μg/ml for bisabolol and menthol or 31.3 μg/ml for thymol. The obtained data indicate that the assayed herbs possessed promising anti-H. pylori bioactivity.

Antimicrobial Activity of Ultrasonic Extracts of Two Chemotypes of Thymus serpyllum L. of Central Kazakhstan and their Polyphenolic Profiles

“…Minimal bactericidal concentration (MBC) or minimal fungicidal concentration (MFC) were obtained by subculturing 5 ml from each well that showed through growth inhibition, from the last positive one and the growth control onto recommended agar plates. The plates were incubated at 35° for 24 h for all microorganisms but H. pylori which was incubated for 72 h in microaerophilic conditions [20], [21]. The MBC/MFC was defined as the lowest concentration of extracts without the growth of microorganisms.…”

Antimicrobial Activity and Polyphenol Profiles of Hydroalcoholic Extracts of Thymus rasitatus Klokov and Thymus eremita Klokov

“…Minimal bactericidal concentration (MBC) or minimal fungicidal concentration (MFC) was obtained by subculturing 5 μl from each well that showed through growth inhibition, from the last positive one and from the growth control onto recommended agar plates. The plates were incubated at 35° for 24 h for all microorganisms but H. pylori which was incubated for 72 h in microaerophilic conditions [11], [12]. The MBC/MFC was defined as the lowest concentration of extracts without growth of microorganisms.…”

Novel in vitro and in vivo anti-Helicobacter pylori effects of pomegranate peel ethanol extract

“…And in modern medicine, these plants represent a rich source for drug discovery [3]. Plant extracts contain bioactive compounds that can be used prophylactically and therapeutically over synthetic drugs [4,5] and may display potent antimicrobial properties [6]. Punica granatum or Pomegranate is one plant with great therapeutic potential.…”

“…Minimum inhibitory concentration of PPEE against clinical strains of Helicobacter pylori(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and the control strain.…”