Anti-hemorrhagic effect of hydro-alcoholic extract of the leaves of Mikania glomerata in lesions induced by Bothrops jararaca venom in rats

Mourao, Vanessa Barreto; Giraldi, Gleice Mara; Neves, Lia Mara Grosso; Gaspi, Fernanda de Gaspari de Oliveira; Rodrigues, Rodney Alexandre Ferreira; Alves, Armindo Antônio; Esquisatto, Marcelo Augusto Marretto; Mazzi, Maurício Ventura; Mendonça, Fernanda Aparecida Sampaio; Santos, Gláucia Maria Tech dos

doi:10.1590/s0102-86502014000100005

Cited by 20 publications

(7 citation statements)

References 30 publications

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“…These findings agree with experimentally identified anti-inflammatory and antihemorrhagic properties of the Mikania genus against myotoxic, neurotoxic, and inflammatory effects of snake venoms [2]. The above observations are further supported by the findings of other studies in rats that revealed 80% reduction in hemorrhage area following intradermal injection of Bothrops venom and Mikania extract administration [14], and reduction of inflammatory cells, edema, and hemorrhagic halo 3 h after administration of B. jararaca venom and Mikania leaf extract [10].…”

supporting

confidence: 90%

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

Motta

Nogueira

Floriano

et al. 2017

Acta Scientiae. Vet.

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Background: Bothropic envenomation represents the most common ophidic accident worldwide, compared to other snakebites of medical interest. Bothropic venom has proteolytic, vasculotoxic, clotting and/or hemorrhagic actions in animals and humans. Mikania glomerata is a plant found in the Brazilian Atlantic Forest with interesting medical properties that may be useful in ameliorating the effects of ophidic venom, and thus, improving response and outcome. Although Mikania is known to act through inhibition of cytolysins in the venom, there is a lack of consistent research data. The aim of this study was to evaluate the effect of M. glomerata in bothropic envenomation treatment. Materials, Methods & Results: Clinical, hematological, biochemical, and histopathological evaluations were performed following Bothropoides jararaca experimental envenomation in three groups of 18 Wistar rats each. Group VS was inoculated in the pelvic limb via intramuscular injection of bothropic venom and received specific anti-venom serum via intraperitoneal injection. Group VSM was similarly inoculated; it received anti-venom serum and a 10% aqueous extract of the Mikania glomerata plant orally. Group C was the control group and received saline solution alone. Evaluations were performed at 0.5 h (M1), 6 h (M2), and 24 h (M3) after venom inoculation. Animals from both inoculated groups (VS and VSM) showed significant clinical alterations (P < 0.05) manifested as discomfort, uneasiness, pain, and severe edema compared to control animals. Animals from inoculated groups also exhibited statistically significant leukocytosis with neutrophilia, and elevation of blood urea nitrogen and creatine kinase until 6 h after inoculation (P < 0.05 compared to control animals). An acute drop in body temperature was observed 6 h after inoculation (P < 0.05). High levels of creatinine were observed at 6 and 24 h, and plasma protein reduction at almost all evaluation time points (P < 0.05) in both groups compared to that in control. Histopathological evaluation of venom-inoculated animals (groups VS and VSM) showed significant renal hydropic degeneration, acute tubular necrosis, congestion, and hemorrhage (P < 0.05 compared to control). In contrast, animals administered plant extract in addition to anti-venom (group VSM) showed milder muscular fiber regeneration and absence of hemorrhage in the inoculated limb, compared to those that received anti-venom alone (group VS). Overall, there were no statistically significant differences between the inoculated groups (P > 0.05) in terms of edema reduction, pain relief, hematological, biochemical, or histopathological alterations. Discussion: Clinical envenomation symptoms can be explained based on previous reports of bothropic events, where cytolysins such as hyaluronidase, phospholipase A2, and esterases are associated with alterations in cell membrane permeability and release of vasoactive agents. Rhabdomyolysis and muscular necrosis are the main causes of muscular and renal alterations in inoculated grou...

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supporting

confidence: 90%

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

Motta

Nogueira

Floriano

et al. 2017

Acta Scientiae. Vet.

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“…Our results are in line with researches that pointed the protective role of M. glomerata in the prevention of the oxidative lung damage in a coal dust exposure model [17] and reduction of inflammatory cells in lesions induced by Bothrops jararaca venom in rats [26].…”

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confidence: 92%

Anti-inflammatory actions of herbal medicines in a model of chronic obstructive pulmonary disease induced by cigarette smoke

Possebon

Lebron

Silva

et al. 2018

Biomedicine & Pharmacotherapy

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The effects of four medicinal herbs (Arctium lappa, Plantago major, Mikania glomerata Spreng and Equisetum arvense) with anti-inflammatory properties were evaluated in a chronic obstructive pulmonary disease (COPD) model. Wistar rats were exposed to cigarette smoke during 8 weeks and one of the groups was orally given a solution containing 4% of each alcoholic herbal extracts during the exposure period. Control group was not exposed to smoke or treated. Histopathological, immunohistochemical and biochemical analyzes were performed. Normal blood plasma levels of gamma glutamyl transferase indicated no toxicity of the administered herbal extracts. The treatment reduced leukocytes influx in bronchoalveolar lavage, mast cell and macrophages numbers in lungs, as well as prevented pulmonary congestion and tracheal metaplasia. Herbal mixture also decreased plasma inflammatory mediator levels and pulmonary expression of annexin A1 and nuclear factor-k?. Our data indicate synergistic and protective effects of the used herbal medicines in animals exposed to cigarette smoke as a potential therapeutic strategy.

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“…Mikania spp. has provided a wide range of promising phamacological activities and it can be described as having expectorant, anti-inflammatory, bronchodilator, anti-hemorrhagic, antiasthmatic, analgesic, anti-mutagenic, trypanocidal, antimicrobial, antifungal, antiulcerogenic, muscle relaxant, and antirheumatic properties (Muelas-Serrano et al, 2000;Paul et al, 2000;Gasparetto et al, 2010;Pérez-Amador et al, 2010;Mourão et al, 2014;Ríos et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Comparative morphoanatomical analysis of Mikania species

Almeida

Hirt

Raeski

et al. 2017

Revista Brasileira de Farmacognosia

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Please cite this article in press as: de Almeida, V.P., et al. Comparative morphoanatomical analysis of Mikania species. Revista Brasileira de Farmacognosia (2016), http://dx. a b s t r a c tMikania belongs to the Asteraceae family and includes a wide range of promising pharmacological activities. Several species of Mikania, which is popularly known in Brazil as "guaco", occur in Southern Brazil and their external morphology is similar. The aim of this study was to investigate the morpho-anatomical characteristics of the leaf and stem of Mikania campanulata, Mikania cordifolia, Mikania glomerata, Mikania hastato-cordata, Mikania microptera and Mikania sessilifolia as a means of providing additional support for differentiating these taxa. The leaves and stems were investigated by employing scanning electron microscopy and light microscopy techniques. The morphological features of Mikania spp. leaves make it possible to differentiate between the species; nevertheless, when the plants were fragmented or pulverized the anatomical features of the leaves and stems supplied additional helpful data in this regard. The main anatomical characteristics were presence of hypodermis and lens shaped epidermal cells, set of trichomes; midrib, petiole and stem shape and vascular pattern; sclerenchymatous ring in the cortex, sclerenchymatous cells and secretory ducts in the pith.

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Anti-hemorrhagic effect of hydro-alcoholic extract of the leaves of Mikania glomerata in lesions induced by Bothrops jararaca venom in rats

Cited by 20 publications

References 30 publications

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

Effects of Mikania glomerata Leaf Extract on Experimental Bothropoides jararaca Envenomation in Wistar Rats

Anti-inflammatory actions of herbal medicines in a model of chronic obstructive pulmonary disease induced by cigarette smoke

Comparative morphoanatomical analysis of Mikania species

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