Anti-Hepatitis C Virus Activity of a Crude Extract from Longan (&lt;i&gt;Dimocarpus longan&lt;/i&gt; Lour.) Leaves

Apriyanto, Dadan Ramadhan; Aoki, Chie; Hartati, Sri; Hanafi, Muhammad; Kardono, Leonardus B.S.; Arsianti, Ade; Louisa, Melva; Sudiro, Tjahjani Mirawati; Dewi, Beti Ernawati; Sudarmono, Pratiwi; Soebandrio, Amin; Hotta, Hak

doi:10.7883/yoken.jjid.2015.107

Cited by 33 publications

(40 citation statements)

References 41 publications

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“…Huh7it cells were cultivated in Dulbeco's Modified Eagle Medium (GIBCO Invitrogen, Carlsbad, CS, USA) supplemented with 10% Fetal Bovine Serum (Biowest, Nualle, France), 0.15 mg/mL Kanamycin (Sigma-Aldrich, St. Louis, MO, USA) and non-essential amino acids (GIBCO-Invitrogen) in 5% CO2 at 37 0 C. The culture cells were cultivated and maintained by periodically re-feeding with new medium. The adapted HCV variant was propagated in Huh7it [27]. Culture supernatant from the infected cells was collected at day 2 and day 5 post infection and concentrated using Amicon Ultra centrifugal filter unit.…”

Section: Cells and Virusesmentioning

confidence: 99%

“…After 48 h incubation, the medium was replaced with MTT reagent containing medium and incubated for 4 h. Absorbance sample was evaluated under microplate reader at 450 and 600 nm, which is correlated with the amount of cell viability. The percentage of cell toxicity was calculated by comparing with untreated cells and further determine its 50% cytotoxic concentration (CC 50 ) values [7,8,27].…”

Section: Mtt Assaymentioning

confidence: 99%

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Antiviral Activities of Curcuma Genus against Hepatitis C Virus

Wahyuni

Permatasari

Widiandani

et al. 2018

Natural Product Communications

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Hepatitis C virus (HCV) infection is one of the major public health problems in the world. Even though the new agents are shown to increase the sustained virology response, however, there are still many people who cannot access the therapy due to the high cost. Moreover, the emergence of resistance and side effects presented the necessity to develop alternative treatment agents for HCV infection. Plants of the genus of curcuma are popular among traditional medicines in the world, including Indonesia. They have been used for many herb remedies and reported to possess many biological activities. Several plants from the curcuma genus were known as treatment agents in liver disease and jaundice. Our current study determines antiviral activities of Curcuma domestica, Curcuma xanthorrhiza, and Curcuma heyneana against HCV and further examines the mechanism of actions. Antiviral activity was performed by in vitro culture cells using Huh 7.5it cells and treated with the mixture of extract and virus JFH1. The effects of extracts in HCV life cycle were determined by mode of action analysis to examine the action of substances in the entry or post entry steps. The results revealed that ethanol extract of C. domestica, C. xanthorrhiza, and C. heyneana showed strong anti-HCV activities with IC 50 values of 1.68 ± 0.05, 4.93 ± 0.42 and 5.49 ± 0.59 µg/mL, respectively without any cytotoxicity effect. Mode of action analysis demonstrated that of C. domestica and C. heyneana exhibit HCV in the entry step, while C. xanthorrhiza inhibit in the entry and post entry steps of HCV life cycle. Docking analysis to predict the interaction of curcumin, the main compound of curcuma genus, revealed a strong interaction between curcumin and 4GAG receptor, a protein involved in the entry step of HCV infection. Moreover, it was also reported to possess good interaction with 4EAW, an HCV NS5B, which plays an important role in HCV replication. These results suggested that C. domestica, C. xanthorrhiza, and C. Heyneana possessed strong inhibition against hepatitis C virus, therefore they may be good candidates for anti-HCV agents.

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Section: Cells and Virusesmentioning

confidence: 99%

Section: Mtt Assaymentioning

confidence: 99%

Antiviral Activities of Curcuma Genus against Hepatitis C Virus

Wahyuni

Permatasari

Widiandani

et al. 2018

Natural Product Communications

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“…The cell line was established by Nakabayashi and Sato J. 25 The human adapted variants of the HCV strain JFH-1 (JFH1a) 26 were inoculated into the Huh7it-1 cells. Culture supernatants were collected at 3-5 days post infection (p.i.…”

Section: Cell Culture and Virus Preparationmentioning

confidence: 99%

“…Cells were treated with fractions 5 or 13 only during or only after viral inoculation for the remaining culture period until viral harvest to assess the mode of action of the plant extracts. 26…”

Section: Evaluation Of Anti-viral Activitiesmentioning

confidence: 99%

Antiviral effect of <em>Archidendron pauciflorum</em> leaves extract to hepatitis C virus: An <em>in vitro</em> study in JFH-1 strain

et al. 2018

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Background: Hepatitis C virus (HCV) is a leading cause of chronic liver diseases. Drug resistance to the regimen is also increasing. Hence, there is a need for new anti-HCV agents that are less toxic and more efficacious. The aim of this study is to evaluate the possibility of A. pauciflorum extracts can be a antiviral drug.Methods: Huh-7it cells were infected with the HCV genotype 2a strain JFH-I in the presence of methanol extracts of Archidenron pauciflorum. The methanol extract further partition used n-hexane, ethyl acetate, n-butanol, and water showed in which butanol extracts exerted the strongest IC50 (6.3 g/ml). Further, the butanol fraction was fractionated and yielded into 13 fractions.Results: The methanol extract of the leaves of A. pauciflorum exhibited concentration dependent inhibition against the JFH1 strain of HCV genotype 2a with an IC50 is 72.5 μg/ml. The butanol fraction exhibited the highest anti-HCV activity with an IC50 is 6.3 μg/ml. The butanol fraction was fractionated which yielded 13 fractions. Fractions 5 and 13 exhibited high anti-HCV activities with IC50 is 5.0 μg/ml and 8.5 μg/ml and a time-of-addition study demonstrated that fraction 5 inhibited viral infection at the post-entry step, whereas fraction 13 primarily inhibited the viral entry step.Conclusion: The extract A. pauciflorum can be used as a herbal-based antiviral drug.

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“…Longan leaves have been reported to contain phenolics, flavonoids, terpenoids and sterols (Yuge, 2012;Xue et al, 2015). The longan leaf extract has shown high antioxidant activity, anti-hepatitis C virus, anti-hyperuricemia and anticancer activity (Rashed and Fouche, 2013;Apriyanto et al, 2016;Sheu et al, 2016). However, there are few reports of antiaging benefits.…”

Section: Introductionmentioning

confidence: 99%

In vitro Biological Activities of the Anti-aging Potential of Dimocarpus longan Leaf Extracts

Doungsaard¹,

Chansakaow²,

Sirithunyalug³

et al. 2020

CMUJNS

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The longan (Dimocarpus longan Lour.) leaves, which are the underutilized part of trimming longan trees to increase their fruit productivity, were of interest in this study. They have been reported to contain phytochemical components which might be used for anti-aging purposes. In this study, longan leaves were extracted using two different solvents including 95% ethanol and 50% ethanol, named ethanolic extract (ET) and hydroethanolic extract (HE), respectively. The extracts were investigated for antioxidation properties, inhibition of hyaluronidase, collagenase, MMP-2 and MMP-9 together with the determination of their total phenolic and flavonoid content. Additionally, HPLC-fingerprinting of the extract was performed. The results revealed that HE had a higher yield with the remarkable property of superior in vitro biological activity compared with ET. HE showed radical scavenging activity on DPPH and hydrogen peroxide with IC50 of 30.03 ± 7.64 and 71.40 ± 15.30 µg/ml, respectively. Moreover, it showed inhibition of lipid peroxidation with IC50 of 537.01 ± 42.32 µg/ml. For inhibition against hyaluronidase and collagenase, HE was detected with IC50 of 234.80 ± 21.52 and 314.44 ± 62.14 µg/ml, respectively. The extract also demonstrated MMP-2 and MMP-9 inhibition, which is more potent than gallic acid as determined by zymography at 1.0 mg/ml. In conclusion, hydroethanolic extract (HE) of longan leaves presented high potential as in vitro antioxidant and inhibitor of enzymatic

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Anti-Hepatitis C Virus Activity of a Crude Extract from Longan (<i>Dimocarpus longan</i> Lour.) Leaves

Cited by 33 publications

References 41 publications

Antiviral Activities of Curcuma Genus against Hepatitis C Virus

Antiviral Activities of Curcuma Genus against Hepatitis C Virus

Antiviral effect of <em>Archidendron pauciflorum</em> leaves extract to hepatitis C virus: An <em>in vitro</em> study in JFH-1 strain

In vitro Biological Activities of the Anti-aging Potential of Dimocarpus longan Leaf Extracts

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