Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Abstract

The rhamnolipid biosurfactant PS-17 and its complex with the polysaccharide alginate, both produced by the Pseudomonas sp. S-17 strain, were studied for their antiviral activity against herpes simplex virus (HSV) types 1 and 2. They significantly inhibited the herpesvirus cytopathic effect (CPE) in the Madin-Darby bovine kidney (MDBK) cell line. The investigations were carried out according to the CPE inhibition assay protocol. The suppressive effect of the compounds on HSV replication was dose-dependent and occurred at concentrations lower than the critical micelle concentration of the surfactant. The 50% inhibitory concentration (IC 50 ) of rhamnolipid PS-17 was 14.5 μg/ml against HSV-1 and 13 μg/ml against HSV-2. The IC 50 values of the complex were 435 μg/ml for HSV-1 and 482 μg/ml for HSV-2. The inhibitory effects of the substances were confirmed by measuring the infectious virus yields with the multicycle virus growth experimental design as well: Δlog CCID 50 of 1.84Ð2.0 against the two types of herpes simplex viruses by rhamnolipid PS-17 (20 μg/ml), and a strong reduction of the HSV-2 virus yield under the effect of the alginate complex at a concentration of 450 μg/ml. The results indicate that rhamnolipid PS-17 and its alginate complex may be considered as promising substances for the development of anti-herpetic compounds.

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

“…Rhamnolipid alginate complex also showed significant antiviral activity against herpes simplex virus types 1 and 2. In particular, they significantly inhibited the herpesvirus cytopathic effect in the Madin-Darby bovine kidney cell line (Remichkova et al, 2008). The suppressive effect of the compounds on herpes simplex virus replication was dosedependent and occurred at concentrations lower than the critical micelle concentration.…”

Microbial biosurfactants as additives for food industries

“…Several biosurfactants display antiadhesive and antimicrobial activities . Zeraik and Nitschke recently reported antiadhesive activity against attachments of Listeria monocytogenes, Staphylococcus aureus, and Micrococcus luteus on polystyrene surfaces using rhamnolipids and surfactin.…”

“…Several biosurfactants display antiadhesive and antimicrobial activities. 133,134 Zeraik and Nitschke 135 recently reported antiadhesive activity against attachments of Listeria monocytogenes, Staphylococcus aureus, and Micrococcus luteus on polystyrene surfaces using rhamnolipids and surfactin. Lunasan produced by the yeast Candida sphaerica UCP0995 also completely inhibited the adhesion of several Streptococcus, Staphylococcus, Pseudomonas, and Candida strains on plastic tissue culture plates.…”

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

“…They are also explored for their antitumor, antiviral and anti-mycoplasmic activities [3,4]. In recent years, biosurfactants are considered as potential drug candidates and looked up for their explicit biomedical applications such as antibacterial, antifungal, antiviral and antiadhesive agents against several pathogens [4][5][6][7][8].…”