Anti-HIV-1 Activities and Chemical Constituents from Leaves and Twigs of <i>Santisukia pagetii</i> (Bignoniaceae)

Limjiasahapong, Suphitcha; Tuchinda, Patoomratana; Reutrakul, Vichai; Pohmakotr, Manat; Akkarawongsapat, Radeekorn; Limthongkul, Jitra; Napaswad, Chanita; Nuntasaen, Narong

doi:10.1177/1934578x1801301109

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…The comparison of IC 50 values in the current study with previous studies showed that the IC 50 values of these anti-HIV-RT drugs against HIV-RT were within the ranges of previous results; 0.084–9.67 µM for NVP, 14–47 nM for EFV, and 15–22 nM for RPV [ 26 27 28 29 30 ]. As the IC 50 values of NVP, EFV, and RPV against FIV-RT were in comparable ranges to HIV-RT, indicating similar abilities of these drugs against both enzymes ( Table 1 and Supplementary Fig.…”

Section: Discussionsupporting

confidence: 86%

Biochemical and structural comparisons of non-nucleoside reverse transcriptase inhibitors against feline and human immunodeficiency viruses

Rattanabunyong,

Choengpanya,

Suwattanasophon

et al. 2023

J Vet Sci

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Background Feline immunodeficiency virus (FIV) causes an acquired immunodeficiency-like syndrome in cats. FIV is latent. No effective treatment has been developed for treatment the infected cats. The first and second generations non-nucleoside reverse transcriptase inhibitors (NNRTIs) for HIV treatment, nevirapine (NVP) and efavirenz (EFV), and rilpivirine (RPV), were used to investigate the potential of NNRTIs for treatment of FIV infection. Objective This study aims to use experimental and in silico approaches to investigate the potential of NNRTIs, NVP, EFV, and RPV, for inhibition of FIV reverse transcriptase (FIV-RT). Methods The FIV-RT and human immunodeficiency virus reverse transcriptase (HIV-RT) were expressed and purified using chromatography approaches. The purified proteins were used to determine the IC 50 values with NVP, EFV, and RPV. Surface plasmon resonance (SPR) analysis was used to calculate the binding affinities of NNRTIs to HIV-RT and FIV-RT. The molecular docking and molecular dynamic simulations were used to demonstrate the mechanism of FIV-RT and HIV-RT with first and second generation NNRTI complexes. Results The IC 50 values of NNRTIs NVP, EFV, and RPV against FIV-RT were in comparable ranges to HIV-RT. The SPR analysis showed that NVP, EFV, and RPV could bind to both enzymes. Computational calculation also supports that these NNRTIs can bind with both FIV-RT and HIV-RT. Conclusions Our results suggest the first and second generation NNRTIs (NVP, EFV, and RPV) could inhibit both FIV-RT and HIV-RT.

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Section: Discussionsupporting

confidence: 86%

Biochemical and structural comparisons of non-nucleoside reverse transcriptase inhibitors against feline and human immunodeficiency viruses

Rattanabunyong,

Choengpanya,

Suwattanasophon

et al. 2023

J Vet Sci

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“…The position of functional groups was also clarified by HBMC correlations (Figure 2). Thus, the structure of 11 was conclusively determined to be 5,7-dihydroxyisocoumarin (Figure 1), and it was named euonymusin C. Repeated column chromatography of n-BuOH soluble fractions led to the isolation of three new compounds, euonymusin A-D (1, 10, and 11); one new set of spectroscopic data that had not previously been reported (2); together with ten known compounds whose chemical structures were identified as those of known compounds by comparing their spectroscopic data with data reported in the literature, namely, quercetin-3-O-β-Dgalactopyranoside (3) [27], quercetin-3,7-di-O-β-D-glucopyranoside (4) [27], isorhamnetin-3,7-di-O-β-D-glucopyranoside (5) [28], quercetin-3-O-sambubioside (6) [29], isoquercetin (7) [30], quercetin-3,3 ′ -di-O-β-D-glucopyranoside (8) [31], liriodendrin (9) [32], caffeic acid trans-3-O-β-D-glucopyranoside (12) [33], methyl trans-3-O-(β-D-glucopyranosyl) caffeate (13) [34], and benzyl gentiobioside (14) [34] (Figure 1).…”

Section: Structural Determination Of Compoundsmentioning

confidence: 99%

Neuroprotective Effects of Chemical Constituents of Leaves of Euonymus hamiltonianus Wall.

Tran,

Yu,

Huang

et al. 2024

Plants

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Euonymus hamiltonianus Wall. is considered a medicinal plant and is used to treat pain, cough, dysuria, and cancer, but a clear phytochemical investigation of its biological activities has yet to be performed. Investigation of chemical constituents of the leaves of Euonymus hamiltonianus Wall. led to the isolation of three new compounds by chromatography techniques, euonymusins A–C (1, 10, and 11), and the acquisition of new spectroscopic data for euonymusin D (2), along with the identification of ten known compounds. The chemical structures of the compounds were established using extensive spectroscopic techniques, including NMR, MS, and hydrolysis, and compared with the published data. These compounds were tested in vitro for their inhibitory effects on beta amyloid production (Aβ42). Compounds 13 and 14 displayed weak inhibition, with IC50 values ranging from 53.15 to 65.43 µM. Moreover, these compounds were also assessed for their inhibitory effects on nitric oxide production. Of these compounds, 3, 4, and 14 displayed inhibitory effects on NO production, with IC50 values ranging from 14.38 to 17.44 µM. Compounds 3, 4, and 14 also suppressed LPS-induced expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein.

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“…An anti-HIV1-RT activity assay, anti-syncytium test and cytotoxic study were done as reported by Pompimon, W., et al [4,5] and Limjiasahapong, S., et al [6].…”

Section: Anti-hivs Assay and Cytotoxic Studymentioning

confidence: 99%

Anti-Syncytium (MC99+1A2) and Anti-Bacterial Activities from Twigs and Stems of Ficus dubia

Pompimon

Udomputtimekakul

Chaisena

et al. 2021

JPRI

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Aims: To study the anti-HIVs and anti-bacterial activity and investigate the chemical compositions of the crude extracts and isolated compounds from stems and twig of Ficus dubia. Methodology: Twigs and stems of Ficus dubia were collected from Si Sawat District, Kanchanaburi Province, Thailand. Bioactivity assay and phytochemical analysis of ficus dubia were processed under standard method including, anti-HIV1-RT, antibacterial activities, and CC chromatography procedures. Structures of the compound were elucidated by spectroscopic techniques. Results: Bioactivities assay exposed that, the hexane, ethyl acetate, and methanol extracts of Ficus dubia were verified for anti-HIV and anti-bacterial activities. The ethyl acetate and methanol extract showed evidence of anti-HIVs-1RT inhibition at 69.02 % and 69.24 %, respectively. Anti-syncytium (MC99+1A2) among evaluated all three extracts: it afforded the lowest EC50; hexane 9.65 (TI 4.39), ethyl acetate 11.15 (TI 3.84), and methanol 43.61 (TI 3.04). Moreover, an antibacterial study on extract was also performed. The antibacterial study was evaluated using nine strains (Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli 0157: H7, Escherichia coli (ETEC), Escherichia coli (EPEC), Proteus mirabilis, Salmonella typhimurium, Shigella flexneri, and Vibrio cholera) by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) method. The MeOH extract was the most effective antibacterial with a MIC in the range >0.1 mg/mL and MBC in the range >0.2 mg/mL. However, the isolated compounds from this plant had not been studied in all bioassay. Phytochemical examination of stems and twigs from Ficus dubia had directed to the isolation of four known compounds, epifriedelanol, β-sitosterol, stigmasterol, and bergapten. Conclusion: The crude extract of twigs and stems of Ficus fubia were specifically active toward MC99+1A2. With these results, it could be concluded that Ficus dubia may be an accomplished candidate in pill and future medicine.

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Anti-HIV-1 Activities and Chemical Constituents from Leaves and Twigs of Santisukia pagetii (Bignoniaceae)

Cited by 4 publications

References 31 publications

Biochemical and structural comparisons of non-nucleoside reverse transcriptase inhibitors against feline and human immunodeficiency viruses

Biochemical and structural comparisons of non-nucleoside reverse transcriptase inhibitors against feline and human immunodeficiency viruses

Neuroprotective Effects of Chemical Constituents of Leaves of Euonymus hamiltonianus Wall.

Anti-Syncytium (MC99+1A2) and Anti-Bacterial Activities from Twigs and Stems of Ficus dubia

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